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引用和文献 (66)
Invitrogen™
钙校准缓冲液试剂盒 #1，零和 10 mM CaEGTA
钙校准缓冲液试剂盒旨在为使用荧光计或定量成像系统测定荧光 Ca2+ 指示剂的解离常数 (Kd) 提供帮助。指示剂的 Kd 可通过扫描指示剂在存在 11 种不同的了解更多信息
| 货号 | 数量 |
|---|---|
| C3008MP | 1 Kit |
货号 C3008MP
价格（CNY)
4,492.00
飞享价
Ends: 31-Dec-2025
6,049.00共减 1,557.00 (26%)
Each
数量:
1 Kit
价格（CNY)
4,492.00
飞享价
Ends: 31-Dec-2025
6,049.00共减 1,557.00 (26%)
Each
钙校准缓冲液试剂盒旨在为使用荧光计或定量成像系统测定荧光 Ca2+ 指示剂的解离常数 (Kd) 提供帮助。指示剂的 Kd 可通过扫描指示剂在存在 11 种不同的 Ca2+ 浓度情况下的激发或发射光而生成的图计算。钙校准试剂盒 #1 含 50 mL 10 mM K2EGTA 和 50 mL 10 mM CaEGTA。两种溶液均含 100 mM KCl 和 30 mM MOPS（pH 值 7.2），且均在去离子水中制备。可将这些储备液混合，以制备游离 Ca2+ 范围为 0 µM 至 39 µM 的缓冲液。
了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›
了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型其他标记或染料
产品规格管、玻片
数量1 Kit
运输条件室温
适用于（应用）钙含量校准
适用于（设备）荧光显微镜, 荧光计
产品类型钙含量校准缓冲液试剂盒
Unit SizeEach
内容与储存
在冰箱（2°C 至 8°C）中储存。
引用和文献 (66)
引用和文献
Abstract
Mechanism of collagen activation in human platelets.
Journal:J Biol Chem
PubMed ID:14981087
The mechanism of collagen-induced human platelet activation was examined using Ca2+, Na+, and the pH-sensitive fluorescent dyes calcium green/fura red, sodium-binding benzofuran isophthalate, and 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Administration of a moderate dose of collagen (10 microg/ml) to human platelets resulted in an increase in [Ca2+](i) and platelet aggregation. The majority of this
The molecular basis of differential subcellular localization of C2 domains of protein kinase C-alpha and group IVa cytosolic phospholipase A2.
Journal:J Biol Chem
PubMed ID:12531893
'The C2 domain is a Ca(2+)-dependent membrane-targeting module found in many cellular proteins involved in signal transduction or membrane trafficking. C2 domains are unique among membrane targeting domains in that they show a wide range of lipid selectivity for the major components of cell membranes, including phosphatidylserine and phosphatidylcholine. To
Differential regulation of nuclear and cytosolic Ca2+ in HeLa cells.
Journal:J Biol Chem
PubMed ID:8940122
'The results reported in this study address the controversial issue that nuclear free Ca2+ ([Ca2+]n) may be regulated independently of cytosolic free Ca2+ ([Ca2+]c). We have measured [Ca2+]n and [Ca2+]c with recombinant aequorin targeted to the nucleus and cytosol in HeLa cells. We found that histamine, ATP, and ionomycin increased
A single optical fiber fluorometric device for measurement of intracellular Ca2+ concentration: its application to hippocampal neurons in vitro and in vivo.
Journal:Neuroscience
PubMed ID:1436506
'We developed a new system to measure the intracellular Ca2+ concentration in the deep region of the central nervous system with a single optical fiber (300 microns in diameter), used for both excitation and detection of the fluorescence of previously loaded fura-2. With this system, a brain region loaded with
Protein and acidosis alter calcium-binding and fluorescence spectra of the calcium indicator indo-1.
Journal:Biophys J
PubMed ID:7819496
'The fluorescent indicator indo-1 is widely used to monitor intracellular calcium concentration. However, quantitation is limited by uncertain effects of the intracellular environment on indicator properties. The goal of this study was to determine the effects of protein and acidosis on the fluorescence spectra and calcium dissociation constant (Kd) of