Mechanism of collagen activation in human platelets.
AuthorsRoberts DE, McNicol A, Bose R
JournalJ Biol Chem
PubMed ID14981087
The mechanism of collagen-induced human platelet activation was examined using Ca2+, Na+, and the pH-sensitive fluorescent dyes calcium green/fura red, sodium-binding benzofuran isophthalate, and 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Administration of a moderate dose of collagen (10 microg/ml) to human platelets resulted in an increase in [Ca2+](i) and platelet aggregation. The majority of this ... More
The growth-related gene product beta induces sphingomyelin hydrolysis and activation of c-Jun N-terminal kinase in rat cerebellar granule neurones.
AuthorsLimatola C, Mileo AM, Giovannelli A, Vacca F, Ciotti MT, Mercanti D, Santoni A, Eusebi F
JournalJ Biol Chem
PubMed ID10593952
'The growth-related gene product beta (GRObeta) is a small chemoattractant cytokine that belongs to the CXC chemokine family, and GRObeta receptors are expressed in the brain, including the cerebellum. We demonstrate that rat cerebellar granule neurones express the GRObeta receptor CXCR2. We also show that, in addition to the known ... More
Intracellular astrocyte calcium waves in situ increase the frequency of spontaneous AMPA receptor currents in CA1 pyramidal neurons.
AuthorsFiacco TA, McCarthy KD
JournalJ Neurosci
PubMed ID14736858
'Spontaneous neurotransmitter release and activation of group I metabotropic glutamate receptors (mGluRs) each play a role in the plasticity of neuronal synapses. Astrocytes may contribute to short- and long-term synaptic changes by signaling to neurons via these processes. Spontaneous whole-cell AMPA receptor (AMPAR) currents were recorded in CA1 pyramidal cells ... More
Control of IP(3)-mediated Ca2+ puffs in Xenopus laevis oocytes by the Ca2+-binding protein parvalbumin.
AuthorsJohn LM, Mosquera-Caro M, Camacho P, Lechleiter JD
JournalJ Physiol
PubMed ID11507154
'1. Elementary events of Ca2+ release (Ca2+ puffs) can be elicited from discrete clusters of inositol 1,4,5 trisphosphate receptors (IP(3)Rs) at low concentrations of IP(3). Ca(2+) puffs have rarely been observed unless elicited by either hormone treatment or introduction of IP(3) into the cell. However, cells appear to have sufficient ... More
Inositol 1,4,5-trisphosphate and ryanodine receptor distributions and patterns of acetylcholine- and caffeine-induced calcium release in cultured mouse hippocampal neurons.
AuthorsSeymour-Laurent KJ, Barish ME
JournalJ Neurosci
PubMed ID7722617
'The distributions of inositol 1,4,5-trisphosphate and ryanodine receptors (InsP3R and RyR) and the patterns of increase in intracellular calcium ion concentration ([Ca2+]i) elicited by their activation were compared in cultured hippocampal neurons. InsP3R and RyR were labeled using specific antibodies and formed small aggregations in the somata and dendrites of ... More
Comparison of subsarcolemmal and bulk calcium concentration during spontaneous calcium release in rat ventricular myocytes.
AuthorsTrafford AW, Díaz ME, O'Neill SC, Eisner DA
JournalJ Physiol
PubMed ID8576849
'1. The aim of these experiments was to compare the time course of changes in intracellular Ca2+ concentration ([Ca2+]i) measured in the bulk cytoplasm with those estimated to occur near the sarcolemma. Sarcolemmal Na(+)-Ca2+ exchange current and [Ca2+]i were measured in single, voltage-clamped ventricular myocytes. 2. Spontaneous Ca2+ release from ... More
Spatiotemporal analysis of calcium dynamics in the nucleus of hamster oocytes.
AuthorsShirakawa H, Miyazaki S
JournalJ Physiol
PubMed ID8814604
'1. Subcellular Ca2+ dynamics inside and around the nucleus of immature hamster oocytes were analysed with confocal Ca2+ imaging. 2. The ratio value between emission intensity of two injected fluorescent Ca2+ indicators, Calcium Green and Fura Red, was almost uniform over the entire oocyte, suggesting that nucleoplasmic Ca2+ concentration ([Ca2+]n) ... More
Modulation of L-type Ca2+ current by fast and slow Ca2+ buffering in guinea pig ventricular cardiomyocytes.
AuthorsYou Y, Pelzer DJ, Pelzer S
JournalBiophys J
PubMed ID8994602
'Free Ca2+ near Ca2+ channel pores is expected to be lower in cardiomyocytes dialyzed with bis-(o-amino-phenoxy)-ethane-N,N,N'',N''-tetraacetic acid (BAPTA) than with ethyleneglycol-bis-(beta-aminoethyl)-N,N,N'',N''-tetraacetic acid (EGTA) because BAPTA chelates incoming Ca2+ more rapidly. The consequences of intracellular Ca2+ buffering by BAPTA (0.2-60 mM) and by EGTA (0.2-67 mM) on whole-cell L-type Ca2+ current ... More
Activity-dependent [Ca2+]i changes in guinea pig vagal motoneurons: relationship to the slow afterhyperpolarization.
AuthorsLasser-Ross N, Ross WN, Yarom Y
JournalJ Neurophysiol
PubMed ID9307116
'Vagal motoneurons in slices from the guinea-pig brain stem were injected with the fluorescent [Ca2+]i indicators fura-2, furaptra, or Calcium Green-1. Spike-induced fluorescence changes were measured in the soma and dendrites and simultaneously the long-lasting afterhyperpolarization was recorded with a sharp microelectrode in the soma. Na+ spikes or Ca2+ spikes ... More
The nitric oxide donor, sodium nitroprusside, increased intranuclear and cytosolic free calcium concentration in single PU5-1.8 cells.
AuthorsKong SK, Choy YM, Lee CY
JournalBiochem Biophys Res Commun
PubMed ID8123018
'In the present study, the effect of sodium nitroprusside (SNP), a nitric oxide (NO) releasing agent, on the intracellular free calcium concentration ([Ca2+]i) was studied. Our results demonstrate that SNP could increase [Ca2+]i in single PU5-1.8 cells, a murine macrophage cell line, as determined by confocal laser scanning microscope with ... More
Differences in the sensitivity to purinergic stimulation of myelinating and non-myelinating Schwann cells in peripheral human and rat nerve.
AuthorsMayer C, Quasthoff S, Grafe P
JournalGlia
PubMed ID9671967
'Schwann cells of the peripheral nervous system are distinguished by morphological and functional criteria in myelinating and non-myelinating subtypes. We and others have previously reported that Schwann cells in isolated peripheral human and rat nerve respond to extracellular application of ATP with a rise in the intracellular free calcium concentration ... More
Cytosolic calcium changes in a process of platelet adhesion and cohesion on a von Willebrand factor-coated surface under flow conditions.
AuthorsKuwahara M, Sugimoto M, Tsuji S, Miyata S, Yoshioka A
JournalBlood
PubMed ID10438701
'Recent flow studies indicated that platelets are transiently captured onto and then translocated along the surface through interaction of glycoprotein (GP) Ib with surface-immobilized von Willebrand factor (vWF). During translocation, platelets are assumed to be activated, thereafter becoming firmly adhered and cohered on the surface. In exploring the mechanisms by ... More
Differing temporal roles of Ca2+ and cAMP in nicotine-elicited elevation of tyrosine hydroxylase mRNA.
AuthorsGueorguiev VD, Zeman RJ, Hiremagalur B, Menezes A, Sabban EL
JournalAm J Physiol
PubMed ID9886920
'The involvement of cAMP- and Ca2+-mediated pathways in the activation of tyrosine hydroxylase (TH) gene expression by nicotine was examined in PC-12 cells. Extracellular Ca2+ and elevations in intracellular Ca2+ concentration ([Ca2+]i) were required for nicotine to increase TH mRNA. The nicotine-elicited rapid rise in [Ca2+]i was inhibited by blockers ... More
Cell-permeant Ca2+ chelators reduce early excitotoxic and ischemic neuronal injury in vitro and in vivo.
AuthorsTymianski M, Wallace MC, Spigelman I, Uno M, Carlen PL, Tator CH, Charlton MP
JournalNeuron
PubMed ID8102532
'We report the characterization of the first successful treatment of neuronal ischemic injury in vivo by cell-permeant Ca2+ chelators. The chelators attenuated glutamate-induced intracellular Ca2+ increases and neurotoxicity in neuronal explant cultures. When infused intravenously in rats, permeant fluorescent BAPTA analogs accumulated in neurons in several brain regions. BAPTA-AM, infused ... More
Modulation of neuronal activity by glial cells in the retina.
AuthorsNewman EA, Zahs KR
JournalJ Neurosci
PubMed ID9592083
'Glial-neuronal communication was studied by monitoring the effect of intercellular glial Ca2+ waves on the electrical activity of neighboring neurons in the eyecup preparation of the rat. Calcium waves in astrocytes and Müller cells were initiated with a mechanical stimulus applied to the retinal surface. Changes in the light-evoked spike ... More
Occurrence and a possible mechanism of penetration of natural killer cells into K562 target cells during the cytotoxic interaction.
AuthorsRadosevic K, van Leeuwen AM, Segers-Nolten IM, Figdor CG, de Grooth BG, Greve J
JournalCytometry
PubMed ID7587714
'The cytotoxic interaction between cloned human Natural Killer (NK) cells and K562 target cells was studied using confocal laser scanning microscopy (CLSM) and conventional fluorescence microscopy. We observed, using fixed as well as living cells, the occurrence of (pseudo) emperipolesis during the interaction. About 30% of conjugated NK cells penetrated, ... More
Matrix regulation of skeletal cell apoptosis. Role of calcium and phosphate ions.
AuthorsAdams CS, Mansfield K, Perlot RL, Shapiro IM
JournalJ Biol Chem
PubMed ID11278303
'Previously, we noted that inorganic phosphate (P(i)), a major component of bone extracellular matrix, induced osteoblast apoptosis (Meleti, Z., Shapiro, I. M., and Adams, C. S. (2000) Bone (NY) 27, 359-366). Since Ca(2+) along with P(i) is released from bone during the resorption process, we advanced the hypothesis that Ca(2+) ... More
Agonist-induced calcium response in single human platelets assayed in a microfluidic device.
'To facilitate drug discovery directed toward platelet-specific targets, we developed a platelet isolation and fluorophore-loading method that yields functionally responsive platelets in which we were able to detect agonist-induced calcium flux using a microfluidics-based screening platform. The platelet preparation protocol was designed to minimize preparation-induced platelet activation and to optimize ... More
Calcium signaling of glial cells along mammalian axons.
AuthorsKriegler S, Chiu SY
JournalJ Neurosci
PubMed ID7692011
'Glial [Ca2+]i signaling was examined in a mammalian white matter lacking neuronal cell bodies and synapses. Rat optic nerves (postnatal days 2 and 7) were stained with calcium indicator dyes and confocal images of [Ca2+bdi were recorded at approximately 25 degrees C or approximately 37 degrees C. Glial cell bodies ... More
Calcium waves in retinal glial cells.
AuthorsNewman EA, Zahs KR
JournalScience
PubMed ID9012354
'Calcium signals were recorded from glial cells in acutely isolated rat retina to determine whether Ca2+ waves occur in glial cells of intact central nervous system tissue. Chemical (adenosine triphosphate), electrical, and mechanical stimulation of astrocytes initiated increases in the intracellular concentration of Ca2+ that propagated at approximately 23 micrometers ... More
Oxytocin-induced desensitization of the oxytocin receptor.
AuthorsRobinson C, Schumann R, Zhang P, Young RC
JournalAm J Obstet Gynecol
PubMed ID12592262
'OBJECTIVE: The purpose of this study was to characterize the oxytocin-induced desensitization of oxytocin-stimulated rises of intracellular calcium in cultured human myocytes. STUDY DESIGN: Culture lines were begun from biopsy specimens of myometrium that had been obtained from women who underwent low transverse cesarean deliveries. Fluorescence changes of calcium green-1 ... More
Two-photon calcium imaging of network activity in XFP-expressing neurons in the mouse.
AuthorsWilson JM, Dombeck DA, Díaz-Ríos M, Harris-Warrick RM, Brownstone RM,
JournalJ Neurophysiol
PubMed ID17303810
'Fluorescent protein (XFP) expression in postnatal neurons allows the anatomical and physiological investigation of identified subpopulations of interneurons with established techniques. However, the spatiotemporal pattern of activity of these XFP neurons within a network and their role in the functional output of the network are more challenging issues to investigate. ... More
Molecular basis of vitamin E action. Tocotrienol potently inhibits glutamate-induced pp60(c-Src) kinase activation and death of HT4 neuronal cells.
AuthorsSen CK, Khanna S, Roy S, Packer L
JournalJ Biol Chem
PubMed ID10777609
'HT4 hippocampal neuronal cells were studied to compare the efficacy of tocopherols and tocotrienol to protect against glutamate-induced death. Tocotrienols were more effective than alpha-tocopherol in preventing glutamate-induced death. Uptake of tocotrienols from the culture medium was more efficient compared with that of alpha-tocopherol. Vitamin E molecules have potent antioxidant ... More
Bcl-2 differentially regulates Ca2+ signals according to the strength of T cell receptor activation.
AuthorsZhong F, Davis MC, McColl KS, Distelhorst CW
JournalJ Cell Biol
PubMed ID16391001
'To investigate the effect of Bcl-2 on Ca2+ signaling in T cells, we continuously monitored Ca2+ concentration in Bcl-2-positive and -negative clones of the WEHI7.2 T cell line after T cell receptor (TCR) activation by anti-CD3 antibody. In Bcl-2-negative cells, high concentrations of anti-CD3 antibody induced a transient Ca2+ elevation, ... More
Fluorescent probes for living cells.
AuthorsJohnson I
JournalHistochem J
PubMed ID10188922
'The functional characteristics of fluorescent probes used for imaging and measuring dynamic processes in living cells are reviewed. Initial consideration is given to general design requirements for delivery, targeting, detectability and fluorescence readout, and current technologies for attaining them. Discussion then proceeds to the more application-specific properties of intracellular ion ... More
Involvement of intracellular or extracellular calcium in activation of tyrosine hydroxylase gene expression in PC12 cells.
AuthorsMenezes A, Zeman R, Sabban E
JournalJ Neurochem
PubMed ID8931463
'The relationship between elevations in intracellular free Ca2+ concentration ([Ca2+]i) by different mechanisms and tyrosine hydroxylase (TH) gene expression was examined. Depolarization by an elevated K+ concentration triggered rapid and sustained increases in [Ca2+]i from a basal level of approximately 50 to 110-150 nM and three- to fourfold elevations in ... More
Sulfhydryl oxidation overrides Mg(2+) inhibition of calcium-induced calcium release in skeletal muscle triads.
AuthorsDonoso P, Aracena P, Hidalgo C
JournalBiophys J
PubMed ID10866954
'We studied the effect of oxidation of sulfhydryl (SH) residues on the inhibition by Mg(2+) of calcium-induced calcium release (CICR) in triad-enriched sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle. Vesicles were either passively or actively loaded with calcium before eliciting CICR by dilution at pCa 4.6-4.4 in the presence ... More
Selection of fluorescent ion indicators for simultaneous measurements of pH and Ca2+.
AuthorsMartínez-Zaguilán R, Parnami G, Lynch RM
JournalCell Calcium
PubMed ID8983854
'The advent of fluorescent ion sensitive indicators has improved our understanding of the mechanisms involved in regulating pHi and [Ca2+]i homeostasis in living cells. However, changes in [Ca2+]i can alter pHi regulatory mechanisms and vice versa, making assignment of either ion to a particular physiological response complex. A further complication ... More
Digital fluorescence imaging of elementary steps of neurosteroid synthesis in rat brain glial cells.
AuthorsKimoto T, Asou H, Ohta Y, Mukai H, Chernogolov AA, Kawato S
JournalJ Pharm Biomed Anal
PubMed ID9226548
'With fluorescence microscopic imaging, we have examined Ca2+ signaling, LDL uptake and distribution of cytochrome P450 scc on individual rat brain glial cells in order to investigate the molecular mechanisms of neurosteroid synthesis. Astrocytes and oligodendrocytes were cultured from newborn rat brain. Ca2+ signaling was observed in Calcium Green-1 loaded ... More
Development of intracellular calcium measurement by time-resolved photon-counting fluorescence.
'Calcium green I, a ratiometric probe based on fluorescence lifetime measurements, was used to monitor intracellular calcium activity ([Ca2+]i) in RINm5F cells using a time-resolved fluorescence confocal microscope. The probe affinity constant has been recalibrated in single cells using ionomycin as a calcium ionophore and ethylenebis(oxyethylenenitrilo)tetraacetic acid as a calcium ... More
Characterization of 3-D collagen hydrogels for functional cell-based biosensing.
AuthorsMao C, Kisaalita WS
JournalBiosens Bioelectron
PubMed ID15018963
'To address the growing demand for functional cell-based assay technologies with accelerated drug discovery applications, we have proposed the use of human neuroblastoma cells (IMR-32) immobilized in three-dimensional (3-D) collagen hydrogel matrices. The gel protects weakly adherent cells from fluid mechanical forces while providing a more physiologically relevant 3-D environment. ... More
Confocal calcium imaging reveals an ionotropic P2 nucleotide receptor in the paranodal membrane of rat Schwann cells.
AuthorsGrafe P, Mayer C, Takigawa T, Kamleiter M, Sanchez-Brandelik R
JournalJ Physiol
PubMed ID10050005
'1. The paranodal Schwann cell region is of major importance for the function of a myelinated axon. In the present study we searched for a possible ionotropic effect of extracellular ATP in this Schwann cell compartment. 2. Whole-cell patch-clamp recordings from cultured rat Schwann cells revealed that ATP and 2''-3''-O-(4-benzoylbenzoyl)-adenosine ... More
In situ imaging of intracellular calcium with ischemia in lung subpleural microvascular endothelial cells.
AuthorsTozawa K, al-Mehdi AB, Muzykantov V, Fisher AB
JournalAntioxid Redox Signal
PubMed ID11228743
'We propose that generation of reactive oxygen species (ROS) during ischemia is associated with an increase in intracellular calcium ([Ca2+]i) in pulmonary capillary endothelial cells. We used an isolated rat lung model and epifluorescence microscopy to evaluate [Ca2+]i in subpleural microvascular endothelial cells in situ by ratio imaging of the ... More
Calcium transients in dendrites of neocortical neurons evoked by single subthreshold excitatory postsynaptic potentials via low-voltage-activated calcium channels.
AuthorsMarkram H, Sakmann B
JournalProc Natl Acad Sci U S A
PubMed ID8197208
'Simultaneous recordings of membrane voltage and concentration of intracellular Ca2+ ([Ca2+]i) were made in apical dendrites of layer 5 pyramidal cells of rat neocortex after filling dendrites with the fluorescent Ca2+ indicator Calcium Green-1. Subthreshold excitatory postsynaptic potentials (EP-SPs), mediated by the activation of glutamate receptor channels, caused a brief ... More
Detecting action potentials in neuronal populations with calcium imaging.
AuthorsSmetters D, Majewska A, Yuste R
JournalMethods
PubMed ID10356353
'The study of neural circuits requires methods for simultaneously recording the activity of populations of neurons. Here, using calcium imaging of neocortical brain slices we take advantage of the ubiquitous distribution of calcium channels in neurons to develop a method to reconstruct the action potentials occurring in a population of ... More
Intercellular calcium signaling and flash photolysis of caged compounds. A sensitive method to evaluate gap junctional coupling.
AuthorsLeybaert L, Sanderson MJ
JournalMethods Mol Biol
PubMed ID11218662
Confocal Ca2+ imaging of organelles, cells, tissues, and organs.
AuthorsWilliams DA, Bowser DN, Petrou S
JournalMethods Enzymol
PubMed ID10506988
P2 purinoceptor-mediated intracellular Ca2+ transients in human sural nerve.
AuthorsWächtler J, Mayer C, Quasthoff S, Adelsberger H, Grafe P
JournalNeuroreport
PubMed ID8817551
Segments of biopsied human sural nerve were stained with the Ca(2+)-sensitive fluorescent dyes Calcium Green-1 and Fura Red. The emission ratio was used to follow changes in the intracellular free Ca2+ concentration ([Ca2+]i). Application of ATP and analogues in concentrations between 0.3 and 300 microM via the bathing solution resulted ... More
Fluorescent histochemical techniques for analysis of intracellular signaling.
AuthorsOksvold MP, Skarpen E, Widerberg J, Huitfeldt HS
JournalJ Histochem Cytochem
PubMed ID11850432
Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques ... More
A custom-made two-photon microscope and deconvolution system.
AuthorsMajewska A, Yiu G, Yuste R
JournalPflugers Arch
PubMed ID11211128
We describe in detail a custom-built two-photon microscope based on a modified confocal scanhead (Olympus Fluoview) and mode-locked Ti:sapphire laser (Coherent Mira 900). This system has internal detectors as well as external whole-field detection and an electrooptical modulator for blanking the beam on flyback and effecting fast changes in excitation ... More
Two-dimensional model of calcium waves reproduces the patterns observed in Xenopus oocytes.
AuthorsGirard S, Lückhoff A, Lechleiter J, Sneyd J, Clapham D
JournalBiophys J
PubMed ID1547335
Biological excitability enables the rapid transmission of physiological signals over distance. Using confocal fluorescence microscopy, we previously reported circular, planar, and spiral waves of Ca2+ in Xenopus laevis oocytes that annihilated one another upon collision. We present experimental evidence that the excitable process underlying wave propagation depends on Ca2+ diffusion ... More
Sustained increase of Ca(+2) oscillations after chronic TRPV1 receptor activation with capsaicin in cultured spinal neurons.
Hyperalgesia and allodynia occur as a consequence of peripheral and central sensitization that follows sustained nociceptive activation. The cellular alterations associated to this state of nociceptive network hyperexcitability represent a form of neuronal plasticity, but they are not well understood because of its complexity in situ. In this study, after ... More
The functional role of the second NPXY motif of the LRP1 beta-chain in tissue-type plasminogen activator-mediated activation of N-methyl-D-aspartate receptors.
AuthorsMartin AM, Kuhlmann C, Trossbach S, Jaeger S, Waldron E, Roebroek A, Luhmann HJ, Laatsch A, Weggen S, Lessmann V, Pietrzik CU,
JournalJ Biol Chem
PubMed ID18321860
The low density lipoprotein receptor-related protein 1 (LRP1) emerges to play fundamental roles in cellular signaling pathways in the brain. One of its prominent ligands is the serine proteinase tissue-type plasminogen activator (tPA), which has been shown to act as a key activator of neuronal mitogen-activated protein kinase pathways via ... More
High efficacy of a Listeria-based vaccine against metastatic breast cancer reveals a dual mode of action.
AuthorsKim SH, Castro F, Paterson Y, Gravekamp C,
JournalCancer Res
PubMed ID19584282
Most cancer vaccines induce CTL responses to tumor-associated antigens (TAA). Killing of tumor cells occurs through TAA-specific CTL-mediated cytolysis. Here, we show that one preventive followed by two therapeutic immunizations with an attenuated Listeria monocytogenes (LM)-based vaccine eradicates all metastases and almost the entire primary tumor in the syngeneic, aggressive ... More
Oscillations of membrane current and excitability driven by metabolic oscillations in heart cells.
AuthorsO'Rourke B, Ramza BM, Marban E
JournalScience
PubMed ID8052856
Periodic changes in membrane ionic current linked to intrinsic oscillations of energy metabolism were identified in guinea pig cardiomyocytes. Metabolic stress initiated cyclical activation of adenosine triphosphate-sensitive potassium current and concomitant suppression of depolarization-evoked intracellular calcium transients. The oscillations in membrane current and excitation-contraction coupling were linked to oscillations in ... More
Ca(2+) dynamics in synaptosomes isolated from the squid optic lobe.
AuthorsBenech JC, Lima PA, Sotelo JR, Brown ER
JournalJ Neurosci Res
PubMed ID11107169
Synaptosomes from the optic lobes of squid (Loligo forbesi) were prepared by homogenization and allowed to settle onto glass coverslips. Synaptosomes were loaded with Ca(2+) sensitive dyes (Fura-2 AM, Calcium Green-1 AM and Calcium Green-5N AM), visualized by light microscopy and Ca(2+) sensitive fluorescence signals recorded and analyzed. With Fura-2, ... More
Measurement of intracellular calcium.
AuthorsTakahashi A, Camacho P, Lechleiter JD, Herman B
JournalPhysiol Rev
PubMed ID10508230
To a certain extent, all cellular, physiological, and pathological phenomena that occur in cells are accompanied by ionic changes. The development of techniques allowing the measurement of such ion activities has contributed substantially to our understanding of normal and abnormal cellular function. Digital video microscopy, confocal laser scanning microscopy, and ... More
Subcellular heterogeneity of mitochondrial membrane potential: relationship with organelle distribution and intercellular contacts in normal, hypoxic and apoptotic cells.
AuthorsDiaz G, Setzu MD, Zucca A, Isola R, Diana A, Murru R, Sogos V, Gremo F
JournalJ Cell Sci
PubMed ID10198289
The subcellular heterogeneity of mitochondrial membrane potential (mDelta psi) was investigated in confluent and sub-confluent cultures of four cell types (human astrocytes, HEp-2, MDCK and Vero cells) in normal growth conditions, hypoxia and apoptosis. The distribution of high-polarized mitochondria, detected by the potential-sensitive probe JC-1, was found to depend on: ... More
Ciliary reorientation is evoked by a rise in calcium level over the entire cilium.
AuthorsIwadate Y, Suzaki T
JournalCell Motil Cytoskeleton
PubMed ID14752804
Internal Ca2+ levels control the pattern of ciliary and flagellar beating in eukaryotes. In ciliates, ciliary reversal is induced by a rise in intra-ciliary Ca2+, but the mechanism by which Ca2+ induces reversal is not known. We injected the fluorescent Ca2+ indicator Calcium Green into a ciliate Didinium nasutum and ... More
Selective stimulation of astrocyte calcium in situ does not affect neuronal excitatory synaptic activity.
Astrocytes are considered the third component of the synapse, responding to neurotransmitter release from synaptic terminals and releasing gliotransmitters--including glutamate--in a Ca(2+)-dependent manner to affect neuronal synaptic activity. Many studies reporting astrocyte-driven neuronal activity have evoked astrocyte Ca(2+) increases by application of endogenous ligands that directly activate neuronal receptors, making ... More
Purinergic receptors have different effects in rat exocrine pancreas. Calcium signals monitored by fura-2 using confocal microscopy.
AuthorsNovak I, Nitschke R, Amstrup J
JournalCell Physiol Biochem
PubMed ID12077553
Pancreatic ducts have several types of purinergic P2 receptors, however, nothing is known about P2 receptors in acini. The aim was to establish whether acini express functional P2 receptors coupled to intracellular Ca2+ signals and to measure the signals ratiometrically in a confocal laser scanning microscope. Rat acini and ducts ... More
Imaging synaptosomal calcium concentration microdomains and vesicle fusion by using total internal reflection fluorescent microscopy.
AuthorsSerulle Y, Sugimori M, Llinás RR
JournalProc Natl Acad Sci U S A
PubMed ID17242349
Transmitter release at chemical synapses is triggered by high calcium concentration microprofiles at the presynaptic cytosol. Such microprofiles, generated by the opening of voltage-dependent calcium channels at the presynaptic plasma membrane, have been defined as calcium concentration microdomains. Using total internal reflection fluorescent microscopy in conjunction with calcium and vesicular ... More
Characterization of the human cysteinyl leukotriene CysLT1 receptor.
AuthorsLynch KR, O'Neill GP, Liu Q, Im DS, Sawyer N, Metters KM, Coulombe N, Abramovitz M, Figueroa DJ, Zeng Z, Connolly BM, Bai C, Austin CP, Chateauneuf A, Stocco R, Greig GM, Kargman S, Hooks SB, Hosfield E, Williams DL, Ford-Hutchinson AW, Caskey CT, Evans JF
JournalNature
PubMed ID10391245
The cysteinyl leukotrienes-leukotriene C4(LTC4), leukotriene D4(LTD4) and leukotriene E4(LTE4)-are important mediators of human bronchial asthma. Pharmacological studies have determined that cysteinyl leukotrienes activate at least two receptors, designated CysLT1 and CysLT2. The CysLT1-selective antagonists, such as montelukast (Singulair), zafirlukast (Accolate) and pranlukast (Onon), are important in the treatment of asthma. ... More
Techniques for measuring and manipulating free Ca2+ in the cytosol and organelles of neutrophils.
AuthorsHallett MB, Hodges R, Cadman M, Blanchfield H, Dewitt S, Pettit EJ, Laffafian I, Davies EV
JournalJ Immunol Methods
PubMed ID10618511
Ca(2+) signalling in neutrophils is important for triggering and coordinating the behaviour of neutrophils. Fluorescent probes for cytosolic free Ca(2+) concentration, e.g., fura2 and fluo3, have been widely used in neutrophils. These probes can be used to monitor Ca(2+) in the cytosol, the nucleus, near the plasma membrane and theoretically ... More
Mechanism of fluorescence and conformational changes of the sarcoplasmic calcium binding protein of the sand worm Nereis diversicolor upon Ca2+ or Mg2+ binding.
AuthorsSillen A, Verheyden S, Delfosse L, Braem T, Robben J, Volckaert G, Engelborghs Y
JournalBiophys J
PubMed ID12944301
The calcium-binding protein isolated from the sarcoplasm of the muscles of the sand worm Nereis diversicolor has four EF-hands and three active binding sites for Ca(2+) or Mg(2+). Nereis diversicolor sarcoplasmic calcium-binding protein contains three tryptophan residues at positions 4, 57, and 170, respectively. The Wt protein shows a very ... More
Neuronal pacemaker for breathing visualized in vitro.
AuthorsKoshiya N, Smith JC
JournalNature
PubMed ID10432113
Breathing movements in mammals arise from a rhythmic pattern of neural activity, thought to originate in the pre-Bötzinger complex in the lower brainstem. The mechanisms generating the neural rhythm in this region are unknown. The central question is whether the rhythm is generated by a network of bursting pacemaker neurons ... More
Neuroligand-mediated calcium signaling in cultured human schwannoma cells.
AuthorsMayer C, Kamleiter M, Sanchez-Brandelik R, Rosenbaum C, Kluwe L, Hanemann CO, Grafe P
JournalJ Peripher Nerv Syst
PubMed ID10442685
Intracellular Ca2+ release regulates proliferation of nonexcitable cells, however, it is not known whether and which neuroligands modulate the free intracellular Ca2+ concentration in human schwannoma cells. Confocal laser scanning microscopy was used for the study of neuroligand-induced Ca2+ signaling in cultured human schwannoma cells loaded with the Ca2+-sensitive fluorescent ... More
Responses of Bergmann glia and granule neurons in situ to N-methyl-D-aspartate, norepinephrine, and high potassium.
AuthorsShao Y, McCarthy KD
JournalJ Neurochem
PubMed ID9166734
To gain insight into neuronal-glial signaling in brain, cerebellar Bergmann glia and granule neurons were studied in acutely isolated slices with the aid of laser scanning confocal microscopy. Both Bergmann glia and granule neurons responded to N-methyl-D-aspartate (NMDA) with a rise in [Ca2+]i. However, the glial NMDA response was frequently ... More
Inositol 1,4,5-trisphosphate directs Ca(2+) flow between mitochondria and the Endoplasmic/Sarcoplasmic reticulum: a role in regulating cardiac autonomic Ca(2+) spiking.
AuthorsJaconi M, Bony C, Richards SM, Terzic A, Arnaudeau S, Vassort G, Pucéat M
JournalMol Biol Cell
PubMed ID10793156
The signaling role of the Ca(2+) releaser inositol 1,4, 5-trisphosphate (IP(3)) has been associated with diverse cell functions. Yet, the physiological significance of IP(3) in tissues that feature a ryanodine-sensitive sarcoplasmic reticulum has remained elusive. IP(3) generated by photolysis of caged IP(3) or by purinergic activation of phospholipase Cgamma slowed ... More
Spatiotemporal pattern of calmodulin and [Ca2+]i is related to resumption of meiosis in mouse oocytes.
AuthorsBi CM, Dai G, Chen Y, Wu YC, Zhang XR, Li CJ
JournalCell Biol Int
PubMed ID15109989
During meiotic maturation, mammalian oocytes undergo a series of morphological and physiological changes that prepare them for fertilization. Calcium-initiated signaling is thought to trigger these processes. In this study, we examine the spatio-temporal pattern of calcium and calmodulin (CaM), its downstream receptor, in order to investigate their association with meiotic ... More
Search for cerebral G cluster neurons responding to taste stimulation with seaweed in Aplysia kurodai by the use of calcium imaging.
AuthorsYoshida R, Nagahama T
JournalJ Neurobiol
PubMed ID12717700
The calcium imaging method can detect the spike activities of many neurons simultaneously. In the present experiments, this method was used to search for unique neurons contributing to feeding behavior in the cerebral ganglia of Aplysia kurodai. We mainly explored the neurons whose cell bodies were located in the G ... More
Glucose availability alters ischaemia-induced changes in intracellular pH and calcium of isolated rat spinal roots.
AuthorsWächtler J, Mayer C, Rucker F, Grafe P
JournalBrain Res
PubMed ID8828583
Peripheral nerves in diabetic patients show an enhanced liability to ischaemic lesions. Using an in vitro model, we have now analysed the possible role of intracellular proton (pHi) and calcium concentrations ([Ca2+]i) for the pathophysiology of this phenomenon. Isolated rat spinal roots were preincubated for 3 to 6 h in ... More
Rapid measurements of intracellular calcium using a fluorescence plate reader.
AuthorsLin K, Sadée W, Quillan JM
JournalBiotechniques
PubMed ID10023544
Intracellular calcium is a universal second messenger that can serve as a broad-based measure of receptor activity. Recent developments in multi-well plate fluorescence readers facilitate measurement of intracellular free-calcium levels and reduce reliance on slower, more cumbersome or expensive data collection methods. In this report, we describe a rapid and ... More
Activation of phospholipase C increases intramembrane electric fields in N1E-115 neuroblastoma cells.
AuthorsXu C, Loew LM
JournalBiophys J
PubMed ID12770917
We imaged the intramembrane potential (a combination of transmembrane, surface, and dipole potential) on N1E-115 neuroblastoma cells with a voltage-sensitive dye. After activation of the B(2) bradykinin receptor, the electric field sensed by the dye increased by an amount equivalent to a depolarization of 83 mV. The increase in intramembrane ... More
Confocal imaging reveals activity-dependent intracellular Ca2+ transients in nociceptive human C fibres.
AuthorsMayer C, Quasthoff S, Grafe P
JournalPain
PubMed ID10431719
Unmyelinated nociceptive fibres are a key element in the human nociceptive system, however, it is very difficult to investigate such fibres in vivo in more detail. An alternate approach are studies on isolated human nerves. Here we describe that confocal Ca2+ imaging reveals new information about the physiology of human ... More
Buffering intracellular calcium disrupts motoneuron development in intact zebrafish embryos.
AuthorsAshworth R, Zimprich F, Bolsover SR
JournalBrain Res Dev Brain Res
PubMed ID11506861
Numerous studies, performed mainly on dissociated cells, have shown that calcium signals have a role during different stages of neuronal development. However, the actions of calcium during neuronal development in vivo remain to be established. The present study has investigated the role of intracellular calcium signals during development of motoneurons ... More
Effect of sub-skinning concentrations of saponin on intracellular Ca2+ and plasma membrane fluidity in cultured cardiac cells.
AuthorsIshida H, Hirota Y, Nakazawa H
JournalBiochim Biophys Acta
PubMed ID8422413
To determine the underlying mechanisms of the positive inotropic effect of sub-skinning concentrations of saponin, we studied changes in the intracellular Ca2+ ([Ca2+]i) and plasma membrane fluidity after exposure to digitonin (a representative saponin) in cultured cardiac cells. [Ca2+]i was measured by use of the fluorescent calcium indicator Calcium Green-1. ... More
Activity-dependent intracellular Ca2+ transients in unmyelinated nerve fibres of the isolated adult rat vagus nerve.
AuthorsWächtler J, Mayer C, Grafe P
JournalPflugers Arch
PubMed ID9479021
Confocal laser scanning microscopy was used to follow changes in the free intracellular calcium concentration ([Ca2+]i) in nerve fibres and adjacent Schwann cells in isolated rat vagus nerves. [Ca2+]i was monitored by the Ca2+-sensitive fluorescent dyes Calcium Green-1 and Fura Red. Intracellular Ca2+ transients were observed during repetitive (1-50 Hz) ... More
Nuclear Ca2+/calmodulin translocation activated by mu-opioid (OP3) receptor.
AuthorsWang D, Tolbert LM, Carlson KW, Sadée W
JournalJ Neurochem
PubMed ID10737597
Previous evidence has suggested a role for calmodulin (CaM) in opioid receptor signaling. We demonstrate here that morphine stimulation of the mu-opioid (OP3) receptor causes rapid CaM translocation to the nucleus in OP3-transfected human embryonic kidney (HEK)-293 cells and in SH-SY5Y human neuroblastoma cells. Ca2+ influx into the cells resulting ... More
Mitochondria shape hormonally induced cytoplasmic calcium oscillations and modulate exocytosis.
AuthorsKaftan EJ, Xu T, Abercrombie RF, Hille B
JournalJ Biol Chem
PubMed ID10835418
Pituitary gonadotropes transduce hormonal input into cytoplasmic calcium ([Ca(2+)](cyt)) oscillations that drive rhythmic exocytosis of gonadotropins. Using Calcium Green-1 and rhod-2 as optical measures of cytoplasmic and mitochondrial free Ca(2+), we show that mitochondria sequester Ca(2+) and tune the frequency of [Ca(2+)](cyt) oscillations in rat gonadotropes. Mitochondria accumulated Ca(2+) rapidly ... More
Biphasic elevation of [Ca(2+)](i) in individual human spermatozoa exposed to progesterone.
AuthorsKirkman-Brown JC, Bray C, Stewart PM, Barratt CL, Publicover SJ
JournalDev Biol
PubMed ID10837122
Fluorimetric studies on progesterone-induced [Ca(2+)](i) signalling in mammalian spermatozoa show both the well-characterised [Ca(2+)](i) transient and a subsequent sustained phase. However, the sustained phase is thought to reflect release of the fluorochrome during the acrosome reaction and has not been subject to critical investigation. We have used single-cell imaging of ... More
Advantages of calcium green-1 over other fluorescent dyes in measuring cytosolic calcium in platelets.
AuthorsLee SK, Lee JY, Lee MY, Chung SM, Chung JH
JournalAnal Biochem
PubMed ID10469489
Fluorescent indicators are widely used in the measurements of cytosolic calcium in many cell types for many purposes because they are relatively easy to use. Notwithstanding, they have some defects to prevent accurate measurements under certain conditions, such as significant dye leakage and UV-quenching effect. Menadione, a representative quinone derivative ... More
InsP3 signaling induces pulse-modulated Ca2+ signals in the nucleus of airway epithelial ciliated cells.
AuthorsQuesada I, Verdugo P
JournalBiophys J
PubMed ID15792976
The phenomenology of nuclear Ca(2+) dynamics has experienced important progress revealing the broad range of cellular processes that it regulates. Although several agonists can mobilize Ca(2+) from storage in the nuclear envelope (NE) to the intranuclear compartment (INC), the mechanisms of Ca(2+) signaling in the nucleus still remain uncertain. Here ... More
A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals.
AuthorsThomas D, Tovey SC, Collins TJ, Bootman MD, Berridge MJ, Lipp P
JournalCell Calcium
PubMed ID11032777
Quantifying the magnitude of Ca2+ signals from changes in the emission of fluorescent indicators relies on assumptions about the indicator behaviour in situ. Factors such as osmolarity, pH, ionic strength and protein environment can affect indicator properties making it advantageous to calibrate indicators within the required cellular or subcellular environment. ... More
Estimating intracellular calcium concentrations and buffering without wavelength ratioing.
AuthorsMaravall M, Mainen ZF, Sabatini BL, Svoboda K
JournalBiophys J
PubMed ID10777761
We describe a method for determining intracellular free calcium concentration ([Ca(2+)]) from single-wavelength fluorescence signals. In contrast to previous single-wavelength calibration methods, the proposed method does not require independent estimates of resting [Ca(2+)] but relies on the measurement of fluorescence close to indicator saturation during an experiment. Consequently, it is ... More
Regulation of calpain activity in rat brain with altered Ca2+ homeostasis.
AuthorsAverna M, Stifanese R, De Tullio R, Passalacqua M, Defranchi E, Salamino F, Melloni E, Pontremoli S
JournalJ Biol Chem
PubMed ID17135258
Activation of calpain occurs as an early event in correlation with an increase in [Ca2+]i induced in rat brain upon treatment with a high salt diet for a prolonged period of time. The resulting sequential events have been monitored in the brain of normal and hypertensive rats of the Milan ... More
Role of calcium in neurotensin-evoked enhancement in firing in mesencephalic dopamine neurons.
AuthorsSt-Gelais F, Legault M, Bourque MJ, Rompré PP, Trudeau LE
JournalJ Neurosci
PubMed ID15014132
Neurotensin (NT) increases neurotransmission within the mesolimbic dopamine system by enhancing the firing rate of dopaminergic (DAergic) neurons and by acting at the nerve terminal level. The signal transduction pathways involved in these effects have not been characterized, but NT receptors are coupled to the phospholipase C pathway and Ca(2+) ... More
Cytosolic Ca2+ changes during in vitro ischemia in rat hippocampal slices: major roles for glutamate and Na+-dependent Ca2+ release from mitochondria.
AuthorsZhang Y, Lipton P
JournalJ Neurosci
PubMed ID10212290
This work determined Ca2+ transport processes that contribute to the rise in cytosolic Ca2+ during in vitro ischemia (deprivation of oxygen and glucose) in the hippocampus. The CA1 striatum radiatum of rat hippocampal slices was monitored by confocal microscopy of calcium green-1. There was a 50-60% increase in fluorescence during ... More
Analysis of Apparent Noncompetitive Responses to Competitive H(1)-Histamine Receptor Antagonists in Fluorescent Imaging Plate Reader-Based Calcium Assays.
AuthorsMiller TR, Witte DG, Ireland LM, Kang CH, Roch JM, Masters JN, Esbenshade TA, Hancock AA
JournalJ Biomol Screen
PubMed ID10838445
We have examined the utility of high throughput fluorescent imaging plate reader (FLIPR)-based calcium assays for pharmacological characterization of G-protein coupled receptors (GPCRs) using recombinant and native human H1-histamine receptors (H(1)-HR), expressed in HEK293 and HeLa S3 cells, respectively, as model systems. For stably transfected HEK293 cell lines, the potency ... More
A novel use for a carbodiimide compound for the fixation of fluorescent and non-fluorescent calcium indicators in situ following physiological experiments.
AuthorsTymianski M, Bernstein GM, Abdel-Hamid KM, Sattler R, Velumian A, Carlen PL, Razavi H, Jones OT
JournalCell Calcium
PubMed ID9105727
The inability to determine the precise intracellular location of non-fluorescent organic calcium chelators such as BAPTA is a persistent problem which has precluded much detailed analysis of the chelators' spatial or temporal dynamics in live cells. Similarly, following physiological experiments with fluorescent indicators like Fura-2, it has often been desirable ... More
Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane.
AuthorsGraier WF, Paltauf-Doburzynska J, Hill BJ, Fleischhacker E, Hoebel BG, Kostner GM, Sturek M
JournalJ Physiol
PubMed ID9481676
1. Endothelial cell activation is correlated with increased cytosolic Ca2+ concentration, often monitored with cytoplasmic Ca2+ dyes, such as fura-2 and Calcium Green-1. We tested the hypothesis that during weak stimulation of porcine coronary artery endothelial cells, focal, subplasmalemmal Ca2+ elevations occur which are controlled by cell membrane Na(+)-Ca2+ exchange ... More
Isolation of the kernel for respiratory rhythm generation in a novel preparation: the pre-Bötzinger complex "island".
AuthorsJohnson SM, Koshiya N, Smith JC
JournalJ Neurophysiol
PubMed ID11287498
The pre-Bötzinger complex (pre-BötC), a bilaterally distributed network of rhythmogenic neurons within the ventrolateral medulla, has been proposed to be the critical locus for respiratory rhythm generation in mammals. To date, thin transverse medullary slice preparations that capture the pre-BötC have served as the optimal experimental model to study the ... More
Tonotopic variations of calcium signalling in turtle auditory hair cells.
AuthorsRicci AJ, Gray-Keller M, Fettiplace R
JournalJ Physiol
PubMed ID10766923
Turtle cochlear hair cells are electrically tuned by a voltage-dependent Ca2+ current and a Ca2+-dependent K+ current (IBK(Ca)). The effects of intracellular calcium buffering on electrical tuning were studied in hair cells at apical and basal cochlear locations tuned to 100 and 300 Hz, respectively. Increasing the intracellular BAPTA concentration ... More
Mechanism by which wortmannin and LY294002 inhibit catecholamine secretion in the rat adrenal medullary cells.
AuthorsWarashina A
JournalCell Calcium
PubMed ID11243932
The effects of wortmannin and LY294002, inhibitors of PI(3)-kinase, in secretagogue-stimulated rat adrenal chromaffin cells loaded with Calcium Green-1 were studied by simultaneously measuring changes in the fluorescence intensity of the indicator (Ca-response) and in the release of catecholamine (secretory response). Before application of these agents, the profile of the ... More
Intercellular and intracellular events following the MHC-unrestricted TCR recognition of a tumor-specific peptide epitope on the epithelial antigen MUC1.
AuthorsMagarian-Blander J, Ciborowski P, Hsia S, Watkins SC, Finn OJ
JournalJ Immunol
PubMed ID9531265
We examined the functional and molecular parameters involved in direct TCR recognition of a tumor-specific peptide epitope on the tumor Ag MUC1. This peptide epitope is tandemly repeated and recognized on the native molecule rather than processed and bound to the MHC. Even though the TCR was not MHC restricted, ... More
Mitochondrial participation in the intracellular Ca2+ network.
AuthorsBabcock DF, Herrington J, Goodwin PC, Park YB, Hille B
JournalJ Cell Biol
PubMed ID9049249
Calcium can activate mitochondrial metabolism, and the possibility that mitochondrial Ca2+ uptake and extrusion modulate free cytosolic [Ca2+] (Cac) now has renewed interest. We use whole-cell and perforated patch clamp methods together with rapid local perfusion to introduce probes and inhibitors to rat chromaffin cells, to evoke Ca2+ entry, and ... More
Privileged access to mitochondria of calcium influx through N-methyl-D-aspartate receptors.
AuthorsPeng TI, Greenamyre JT
JournalMol Pharmacol
PubMed ID9614198
Mitochondrial Ca2+ uptake responds dynamically and sensitively to changes in cytosolic Ca2+ levels and plays a crucial role in sequestering the large Ca2+ load induced by N-methyl-D-aspartate (NMDA) receptor activation. However, the precise interrelationships between NMDA receptor activation, cytosolic Ca2+ increase, and mitochondrial Ca2+ uptake remain obscure. To reliably, independently, ... More
Alterations in cellular Ca(2+) and free iron pool by sulfur amino acid deprivation: the role of ferritin light chain down-regulation in prooxidant production.
AuthorsKim HJ, Kim SG
JournalBiochem Pharmacol
PubMed ID11992632
Deficiency of sulfur amino acids occurs in certain pathophysiological states such as protein-calorie malnutrition. Sulfur amino acid deprivation (SAAD) increases oxidative stress through a decrease in GSH. Ferritin expression is induced by oxidative stress, which confers resistance to oxidative insults. The effects of SAAD on the changes in cellular Ca(2+) ... More
Local and cellular Ca2+ transients in smooth muscle of pressurized rat resistance arteries during myogenic and agonist stimulation.
AuthorsMiriel VA, Mauban JR, Blaustein MP, Wier WG
JournalJ Physiol
PubMed ID10420017
1. Confocal laser scanning microscopy was used to visualize Ca2+ transients in the vascular smooth muscle cells (VSMC) of intact, pressurized rat mesenteric resistance arteries loaded with fluorescent calcium indicators. Vasoconstriction was assessed by measuring inner arterial diameter. All arteries were studied at 70 mmHg intralumenal pressure and 37 C. ... More
Mitochondrial free calcium levels (Rhod-2 fluorescence) and ultrastructural alterations in neuronally differentiated PC12 cells during ceramide-dependent cell death.
AuthorsMuriel MP, Lambeng N, Darios F, Michel PP, Hirsch EC, Agid Y, Ruberg M
JournalJ Comp Neurol
PubMed ID10982470
Mitochondrial free calcium levels measured by Rhod-2 fluorescence and ultrastructure were examined during cell death in nerve growth factor (NGF)-differentiated PC12 cells that were 1) exposed to C2-ceramide, 2) deprived of serum to induce endogenous ceramide production, or 3) treated with calcium ionophore A23187. Rhod-2 fluorescence in mitochondria and also ... More
Electromechanical coupling between skeletal and cardiac muscle. Implications for infarct repair.
AuthorsReinecke H, MacDonald GH, Hauschka SD, Murry CE
JournalJ Cell Biol
PubMed ID10791985
Skeletal myoblasts form grafts of mature muscle in injured hearts, and these grafts contract when exogenously stimulated. It is not known, however, whether cardiac muscle can form electromechanical junctions with skeletal muscle and induce its synchronous contraction. Here, we report that undifferentiated rat skeletal myoblasts expressed N-cadherin and connexin43, major ... More
The mycotoxin phomoxanthone A disturbs the form and function of the inner mitochondrial membrane.
Authors
JournalCell Death Dis
PubMed ID29459714
LETM1 haploinsufficiency causes mitochondrial defects in cells from humans with Wolf-Hirschhorn syndrome: implications for dissecting the underlying pathomechanisms in this condition.
Authors
JournalDis Model Mech
PubMed ID24626991
IL-1β reciprocally regulates chemokine and insulin secretion in pancreatic β-cells via NF-κB.
Authors
JournalAm J Physiol Endocrinol Metab
PubMed ID26306596
Culturing embryonic nasal explants for developmental and physiological study.
Authors
JournalCurr Protoc Neurosci
PubMed ID22470149
Amide-type local anesthetics and human mesenchymal stem cells: clinical implications for stem cell therapy.
Authors
JournalStem Cells Transl Med
PubMed ID24436443
A mechanism underlying the effects of polyunsaturated fatty acids on breast cancer.
Authors
JournalInt J Mol Med
PubMed ID22692672
Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis.
Authors
JournalFront Plant Sci
PubMed ID28111589
Mechanism of pyrethroid pesticide-induced apoptosis: role of calpain and the ER stress pathway.