Click-IT™ GlcNAz 代谢糖蛋白标记试剂（四乙酰化 N-叠氮基乙酰葡糖胺）
Click-IT™ GlcNAz 代谢糖蛋白标记试剂（四乙酰化 N-叠氮基乙酰葡糖胺）
引用和文献 (4)
Invitrogen™

Click-IT™ GlcNAz 代谢糖蛋白标记试剂（四乙酰化 N-叠氮基乙酰葡糖胺）

Green features
Click-iT™ GlcNAz 代谢糖蛋白标记试剂提供了一种简单而可靠的两步法技术的第一部分，可用于鉴定和表征细胞内 O-GlcNAc 糖蛋白。在第一步中，使用叠氮化物修饰的葡糖胺 (GlcNAc) 与培养的细胞共同孵育。叠氮基糖通过寡糖生物合成途径的允许性质掺入细胞内含 O-GlcNAc了解更多信息
Have Questions?
货号数量
C333675.2 mg
货号 C33367
价格（CNY)
4,159.00
飞享价
Ends: 31-Dec-2025
5,249.00
共减 1,090.00 (21%)
Each
添加至购物车
数量:
5.2 mg
价格（CNY)
4,159.00
飞享价
Ends: 31-Dec-2025
5,249.00
共减 1,090.00 (21%)
Each
添加至购物车
Click-iT™ GlcNAz 代谢糖蛋白标记试剂提供了一种简单而可靠的两步法技术的第一部分，可用于鉴定和表征细胞内 O-GlcNAc 糖蛋白。在第一步中，使用叠氮化物修饰的葡糖胺 (GlcNAc) 与培养的细胞共同孵育。叠氮基糖通过寡糖生物合成途径的允许性质掺入细胞内含 O-GlcNAc 的糖蛋白中。在第二步中，通过叠氮化物和炔烃之间的化学选择性连接或 click 反应，使用 Click-iT™ 糖蛋白检测试剂盒检测凝胶（TAMRA 或 Dapoxyl™ 炔烃）或蛋白质印迹（生物素炔烃）的叠氮基标记糖蛋白。Click-iT™ 糖蛋白产品与 LC-MS⁄MS 及 Multiplexed Proteomics™ 技术兼容，可用于糖蛋白组学的深度分析。
仅供科研使用。不可用于诊断程序。
规格
检测方法基于生物素，荧光
环保功能Less hazardous
产品线Click-iT
产品类型标记试剂
数量5.2 mg
运输条件室温
标记目标蛋白质
标签或染料Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, 生物素, Oregon Green 488, TMR（四甲基罗丹明）
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中储存。

引用和文献 (4)

引用和文献
Abstract
Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics.
Authors:Laughlin ST, Agard NJ, Baskin JM, Carrico IS, Chang PV, Ganguli AS, Hangauer MJ, Lo A, Prescher JA, Bertozzi CR,
Journal:Methods Enzymol
PubMed ID:17116478
'The staggering complexity of glycans renders their analysis extraordinarily difficult, particularly in living systems. A recently developed technology, termed metabolic oligosaccharide engineering, enables glycan labeling with probes for visualization in cells and living animals, and enrichment of specific glycoconjugate types for proteomic analysis. This technology involves metabolic labeling of glycans ... More
Dynamic monitoring of newly synthesized proteomes: up-regulation of myristoylated protein kinase A during butyric acid induced apoptosis.
Authors:Liu K, Yang PY, Na Z, Yao SQ,
Journal:Angew Chem Int Ed Engl
PubMed ID:21678537
Doubly charged: A double metabolic incorporation approach capable of proteome-wide profiling of post-translational modification dynamics on newly synthesized proteins has been developed (see scheme; blue box: methionine surrogate, orange diamond: PTM probe). This strategy reveals for the first time that up-regulation of myristoylated PKA protein is necessary for the occurrence ... More
The chemical neurobiology of carbohydrates.
Authors:Murrey HE, Hsieh-Wilson LC,
Journal:Chem Rev
PubMed ID:18452339
The problems associated with oligosaccharide analysis have hindered efforts to understand the biology of oligosaccharides yet have given chemists a unique opportunity to develop new methods to overcome these challenges. The development of chemical tools for the analysis of glycan structure and function is essential to advance our understanding of ... More
A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly.
Authors:Ohn T, Kedersha N, Hickman T, Tisdale S, Anderson P,
Journal:Nat Cell Biol
PubMed ID:18794846
Stress granules (SGs) and processing bodies (PBs) are microscopically visible ribonucleoprotein granules that cooperatively regulate the translation and decay of messenger RNA. Using an RNA-mediated interference-based screen, we identify 101 human genes required for SG assembly, 39 genes required for PB assembly, and 31 genes required for coordinate SG and ... More