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引用和文献 (6)
Invitrogen™
Click-iT™ 生物素蛋白质分析检测试剂盒
Click-iT™ 生物素糖蛋白检测试剂盒提供了一种简单而可靠技术的第二部分，可用于通过蛋白质印迹鉴定和表征糖蛋白。在第二步中，使用 Click-iT™ 代谢标记试剂或 Click-iT™ 酶促标记体系将叠氮化物基团掺入蛋白质聚糖结构之后，通过叠氮化物和炔烃之间的化学选择性连接或 click 反应检测叠氮化物修饰的糖蛋白。该技术的检测灵敏度在较低的飞摩尔范围内，并且可以在使用一抗进行蛋白质标记之前或之后检测生物素标记的样品了解更多信息
| 货号 | 数量 |
|---|---|
| C33372 | 1 个试剂盒 |
货号 C33372
价格（CNY)
5,116.00
Each
数量:
1 个试剂盒
价格（CNY)
5,116.00
Each
Click-iT™ 生物素糖蛋白检测试剂盒提供了一种简单而可靠技术的第二部分，可用于通过蛋白质印迹鉴定和表征糖蛋白。在第二步中，使用 Click-iT™ 代谢标记试剂或 Click-iT™ 酶促标记体系将叠氮化物基团掺入蛋白质聚糖结构之后，通过叠氮化物和炔烃之间的化学选择性连接或 click 反应检测叠氮化物修饰的糖蛋白。该技术的检测灵敏度在较低的飞摩尔范围内，并且可以在使用一抗进行蛋白质标记之前或之后检测生物素标记的样品。
仅供科研使用。不可用于诊断程序。
规格
检测方法基于生物素，荧光
环保功能Less hazardous
产品线Click-iT
产品类型生物素蛋白分析检测试剂盒
数量1 个试剂盒
运输条件室温
标记目标蛋白质
标签或染料生物素
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中储存。
引用和文献 (6)
引用和文献
Abstract
Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
Journal:J Am Chem Soc
PubMed ID:18683930
'We report an advanced chemoenzymatic labeling strategy for direct fluorescence detection of O-GlcNAc proteins in gels that facilitates proteomic studies and greatly extend the reach of existing technologies. These new tools also enable the expression and dynamics of O-GlcNAc modifications to be monitored by imaging in cells and tissues.
Comparative methods for analysis of protein covalent modification by electrophilic quinoids formed from xenobiotics.
Journal:Bioconjug Chem
PubMed ID:19301905
'Conjugation of biotin and fluorophore tags is useful for assaying covalent protein modification. Oxidative bioactivation of selective estrogen receptor modulators (SERMs) yields reactive quinoid electrophiles that covalently modify proteins, and bioactivation is associated with carcinogenic and chemopreventive effects. Identification of the protein targets of electrophilic metabolites is of general importance
Rapid temporal dynamics of transcription, protein synthesis, and secretion during macrophage activation.
Journal:
PubMed ID:24396086
Macrophages provide the first line of host defense with their capacity to react to an array of cytokines and bacterial components requiring tight regulation of protein expression and secretion to invoke a properly tuned innate immune response. To capture the dynamics of this system, we introduce a novel method combining
A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly.
Journal:Nat Cell Biol
PubMed ID:18794846
Stress granules (SGs) and processing bodies (PBs) are microscopically visible ribonucleoprotein granules that cooperatively regulate the translation and decay of messenger RNA. Using an RNA-mediated interference-based screen, we identify 101 human genes required for SG assembly, 39 genes required for PB assembly, and 31 genes required for coordinate SG and
Identification of structural and functional O-linked N-acetylglucosamine-bearing proteins in Xenopus laevis oocyte.
Journal:Mol Cell Proteomics
PubMed ID:18617508
O-Linked N-acetylglucosaminylation (O-GlcNAcylation) (or O-linked N-acetylglucosamine (O-GlcNAc)) is an abundant and reversible glycosylation type found within the cytosolic and the nuclear compartments. We have described previously the sudden O-GlcNAcylation increase occurring during the Xenopus laevis oocyte G(2)/M transition, and we have demonstrated that the inhibition of O-GlcNAc-transferase (OGT) blocked this