Method for assessment of viability and morphological changes of bacteria in the early stage of colony formation on a simulated natural environment.
AuthorsShimomura Y, Ohno R, Kawai F, Kimbara K
JournalAppl Environ Microbiol
PubMed ID16820503
'A quantitative analysis of changes in the physiological status of bacterial cells is a fundamental type of study in microbiological research. We devised a method for measuring the viability of bacteria in the early stage of colony formation on a simulated natural environment. In this method, a solid medium containing ... More
Functional capacity of Mycobacterium tuberculosis-specific T cell responses in humans is associated with mycobacterial load.
AuthorsDay CL, Abrahams DA, Lerumo L, Janse van Rensburg E, Stone L, O'rie T, Pienaar B, de Kock M, Kaplan G, Mahomed H, Dheda K, Hanekom WA,
JournalJ Immunol
PubMed ID21775682
'High Ag load in chronic viral infections has been associated with impairment of Ag-specific T cell responses; however, the relationship between Ag load in chronic Mycobacterium tuberculosis infection and functional capacity of M. tuberculosis-specific T cells in humans is not clear. We compared M. tuberculosis-specific T cell-associated cytokine production and ... More
Three-dimensional culture for expansion and differentiation of mouse embryonic stem cells.
AuthorsLiu H, Collins SF, Suggs LJ,
JournalBiomaterials
PubMed ID16860386
'Differentiation of embryonic stem (ES) cells typically requires cell-cell aggregation in the form of embryoid bodies (EBs). This process is not very well controlled and final cell numbers can be limited by EB agglomeration and the inability to drive differentiation towards a desired cell type. This study compares three-dimensional (3D) ... More
Myosin light chain kinase mediates transcellular intravasation of breast cancer cells through the underlying endothelial cells: a three-dimensional FRET study.
'The transient and localized signaling events between invasive breast cancer cells and the underlying endothelial cells have remained poorly characterized. We report a novel approach integrating vascular engineering with three-dimensional time-lapse fluorescence resonance energy transfer (FRET) imaging to dissect how endothelial myosin light chain kinase (MLCK) is modulated during tumor ... More