Appraising the suitability of succinimidyl and lipophilic fluorescent dyes to track proliferation in non-quiescent cells by dye dilution.
AuthorsFilby A, Begum J, Jalal M, Day W,
Journal
PubMed ID25802116
Successful completion of the cell cycle usually results in two identical daughter progeny. This process of generational doubling is termed proliferation and when it occurs in a regulated fashion the benefits range from driving embryonic development to mounting a successful immune response. However when it occurs in a dis-regulated fashion, ... More
Isolation and Activation of Murine Lymphocytes.
AuthorsLim JF, Berger H, Su IH
JournalJ Vis Exp
PubMed ID27842342
'B and T cells, with their extremely diverse antigen-receptor repertoires, have the ability to mount specific immune responses against almost any invading pathogen(1,2). Understandably, such intricate abilities are controlled by a large number of molecules involved in various cellular processes to ensure timely and spatially regulated immune responses(3). Here, we ... More