Cocaine evokes projection-specific synaptic plasticity of lateral habenula neurons.
AuthorsMaroteaux M, Mameli M,
JournalJ Neurosci
PubMed ID22956853
Addictive drugs share the ability to increase dopamine (DA) levels and trigger synaptic adaptations in the mesocorticolimbic system, two cellular processes engaged in the early stages of drug seeking. Neurons located in the lateral habenula (LHb) modulate the activity of DA neurons and DA release, and adaptively tune goal-directed behaviors. ... More
Intracellular trafficking of Clostridium perfringens iota-toxin b.
AuthorsNagahama M, Umezaki M, Tashiro R, Oda M, Kobayashi K, Shibutani M, Takagishi T, Ishidoh K, Fukuda M, Sakurai J,
JournalInfect Immun
PubMed ID22825447
'Clostridium perfringens iota-toxin is composed of an enzymatic component (Ia) and a binding component (Ib). Ib binds to a cell surface receptor, undergoes oligomerization in lipid rafts, and binds Ia. The resulting complex is then endocytosed. Here, we show the intracellular trafficking of iota-toxin. After the binding of the Ib ... More
The B cell-specific major raft protein, Raftlin, is necessary for the integrity of lipid raft and BCR signal transduction.
AuthorsSaeki K, Miura Y, Aki D, Kurosaki T, Yoshimura A
JournalEMBO J
PubMed ID12805216
'Recent evidence indicates that membrane microdomains, termed lipid rafts, have a role in B-cell activation as platforms for B-cell antigen receptor (BCR) signal initiation. To gain an insight into the possible functioning of lipid rafts in B cells, we applied liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) methodologies to ... More
Lipid raft microdomains: a gateway for compartmentalized trafficking of Ebola and Marburg viruses.
AuthorsBavari S, Bosio CM, Wiegand E, Ruthel G, Will AB, Geisbert TW, Hevey M, Schmaljohn C, Schmaljohn A, Aman MJ
JournalJ Exp Med
PubMed ID11877482
'Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, ... More
Identification and characterization of small molecules that inhibit intracellular toxin transport.
AuthorsSaenz JB, Doggett TA, Haslam DB
JournalInfect Immun
PubMed ID17576758
'Shiga toxin (Stx), cholera toxin (Ctx), and the plant toxin ricin are among several toxins that reach their intracellular destinations via a complex route. Following endocytosis, these toxins travel in a retrograde direction through the endosomal system to the trans-Golgi network, Golgi apparatus, and endoplasmic reticulum (ER). There the toxins ... More
Specialized cortical subnetworks differentially connect frontal cortex to parahippocampal areas.
AuthorsHirai Y, Morishima M, Karube F, Kawaguchi Y,
JournalJ Neurosci
PubMed ID22302828
'How information is manipulated and segregated within local circuits in the frontal cortex remains mysterious, in part because of inadequate knowledge regarding the connectivity of diverse pyramidal cell subtypes. The frontal cortex participates in the formation and retrieval of declarative memories through projections to the perirhinal cortex, and in procedural ... More
High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine.
AuthorsFairn GD, Schieber NL, Ariotti N, Murphy S, Kuerschner L, Webb RI, Grinstein S, Parton RG,
JournalJ Cell Biol
PubMed ID21788369
'Phosphatidylserine (PS) plays a central role in cell signaling and in the biosynthesis of other lipids. To date, however, the subcellular distribution and transmembrane topology of this crucial phospholipid remain ill-defined. We transfected cells with a GFP-tagged C2 domain of lactadherin to detect by light and electron microscopy PS exposed ... More
A neural circuit for memory specificity and generalization.
AuthorsXu W, Südhof TC,
JournalScience
PubMed ID23493706
'Increased fear memory generalization is associated with posttraumatic stress disorder, but the circuit mechanisms that regulate memory specificity remain unclear. Here, we define a neural circuit-composed of the medial prefrontal cortex, the nucleus reuniens (NR), and the hippocampus-that controls fear memory generalization. Inactivation of prefrontal inputs into the NR or ... More
Ephrin-As and neural activity are required for eye-specific patterning during retinogeniculate mapping.
'In mammals, retinal ganglion cell (RGC) projections initially intermingle and then segregate into a stereotyped pattern of eye-specific layers in the dorsal lateral geniculate nucleus (dLGN). Here we found that in mice deficient for ephrin-A2, ephrin-A3 and ephrin-A5, eye-specific inputs segregated but the shape and location of eye-specific layers were ... More
Glucoprivation in the ventrolateral medulla decreases brown adipose tissue sympathetic nerve activity by decreasing the activity of neurons in raphe pallidus.
AuthorsMadden CJ,
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID22071154
'In urethane/a-chloralose anesthetized rats, cold exposure increased brown adipose tissue sympathetic nerve activity (BAT SNA: +699 ± 104% control). Intravenous administration of 2-deoxy-D-glucose (2-DG; 200 mg·ml(-1)·kg(-1)) reversed the cold-evoked activation of BAT SNA (nadir: 139 ± 36% of control) and decreased BAT temperature (-1.1 ± 0.2°C), expired CO(2) (-0.4 ± ... More
Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells.
AuthorsLuo Q, Kumar P, Vickers TJ, Sheikh A, Lewis WG, Rasko DA, Sistrunk J, Fleckenstein JM,
Journal
PubMed ID24478067
'Enterotoxigenic Escherichia coli (ETEC) is a leading cause of death due to diarrheal illness among young children in developing countries, and there is currently no effective vaccine. Many elements of ETEC pathogenesis are still poorly defined. Here we demonstrate that YghJ, a secreted ETEC antigen identified in immunoproteomic studies using ... More
The efficacy of the fluorescent conjugates of cholera toxin subunit B for multiple retrograde tract tracing in the central nervous system.
AuthorsConte WL, Kamishina H, Reep RL,
JournalBrain Struct Funct
PubMed ID19621243
'Cholera toxin subunit B (CTB) is a sensitive neuroanatomical tracer that generally transports retrogradely in the nervous system, and has been used extensively in brightfield microscopy. Recently, Alexa Fluor (AF) conjugates of CTB have been made available, which now allows multiple tracing with CTB. In this study, we examined the ... More
Retrograde labeling of phrenic motoneurons by intrapleural injection.
AuthorsMantilla CB, Zhan WZ, Sieck GC,
JournalJ Neurosci Methods
PubMed ID19559048
'Studies of motoneuron plasticity during development or in response to injury or disease rely on the ability to correctly identify motoneurons innervating specific muscle groups. Commonly, injections of retrograde tracer molecules into a target muscle or into a transected nerve are used to label specific motoneuron pools. However, intramuscular injection ... More
Decoupling eye-specific segregation from lamination in the lateral geniculate nucleus.
AuthorsHuberman AD, Stellwagen D, Chapman B
JournalJ Neurosci
PubMed ID12417667
'To determine whether there is a critical period for development of eye-specific layers in the lateral geniculate nucleus (LGN), we prevented the normal segregation of retinogeniculate afferents and then allowed an extended period of time for recovery. After recovery, both anatomy and physiology revealed strictly nonoverlapping territories of input from ... More
Tracing from fat tissue, liver, and pancreas: a neuroanatomical framework for the role of the brain in type 2 diabetes.
AuthorsKreier F, Kap YS, Mettenleiter TC, van Heijningen C, van der Vliet J, Kalsbeek A, Sauerwein HP, Fliers E, Romijn JA, Buijs RM
JournalEndocrinology
PubMed ID16339209
'The hypothalamus uses hormones and the autonomic nervous system to balance energy fluxes in the body. Here we show that the autonomic nervous system has a distinct organization in different body compartments. The same neurons control intraabdominal organs (intraabdominal fat, liver, and pancreas), whereas sc adipose tissue located outside the ... More
Crossed and uncrossed retinal projections to the hamster circadian system.
AuthorsMuscat L, Huberman AD, Jordan CL, Morin LP
JournalJ Comp Neurol
PubMed ID14566946
'The hamster suprachiasmatic nucleus (SCN), site of the circadian clock, has been thought to be equally and completely innervated by each retina. This issue was studied in animals that had received an injection of the tracer cholera toxin subunit B (CTb) conjugated to Alexa 488 into the vitreous of one ... More
Spontaneous activity promotes synapse formation in a cell-type-dependent manner in the developing retina.
AuthorsSoto F, Ma X, Cecil JL, Vo BQ, Culican SM, Kerschensteiner D,
JournalJ Neurosci
PubMed ID22514306
'Spontaneous activity is thought to regulate synaptogenesis in many parts of the developing nervous system. In vivo evidence for this regulation, however, is scarce and comes almost exclusively from experiments in which normal activity was reduced or blocked completely. Thus, whether spontaneous activity itself promotes synaptogenesis or plays a purely ... More
The dependence receptor DCC requires lipid raft localization for cell death signaling.
AuthorsFurne C, Corset V, Hérincs Z, Cahuzac N, Hueber AO, Mehlen P
JournalProc Natl Acad Sci U S A
PubMed ID16537496
'DCC (deleted in colorectal cancer) is a putative tumor suppressor gene whose expression is lost in numerous cancers. DCC also encodes the main receptor for the neuronal navigation cue netrin-1. It has been shown that DCC belongs to the so-called family of dependence receptors. Such receptors induce apoptosis when their ... More
High-resolution mapping of anatomical connections in marmoset extrastriate cortex reveals a complete representation of the visual field bordering dorsal V2.
AuthorsJeffs J, Federer F, Ichida JM, Angelucci A,
JournalCereb Cortex
PubMed ID22523183
The primate visual cortex consists of many areas. The posterior areas (V1, V2, V3, and middle temporal) are thought to be common to all primate species. However, the organization of cortex immediately anterior to area V2 (the ... More
Serial two-photon tomography for automated ex vivo mouse brain imaging.
AuthorsRagan T, Kadiri LR, Venkataraju KU, Bahlmann K, Sutin J, Taranda J, Arganda-Carreras I, Kim Y, Seung HS, Osten P,
JournalNat Methods
PubMed ID22245809
Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical ... More
Multiple neuroanatomical tract-tracing using fluorescent Alexa Fluor conjugates of cholera toxin subunit B in rats.
AuthorsConte WL, Kamishina H, Reep RL,
JournalNat Protoc
PubMed ID19617887
Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical connections can be reliably studied and compared in the same animal. Here we provide a protocol that describes the use of AF-CTB for studying ... More
High frequency, synchronized bursting drives eye-specific segregation of retinogeniculate projections.
AuthorsTorborg CL, Hansen KA, Feller MB
JournalNat Neurosci
PubMed ID15608630
Blockade of retinal waves prevents the segregation of retinogeniculate afferents into eye-specific layers in the visual thalamus. However, the key features of retinal waves that drive this refinement are controversial. Some manipulations of retinal waves lead to normal eye-specific segregation but others do not. By comparing retinal spiking patterns in ... More
Vav family GEFs link activated Ephs to endocytosis and axon guidance.
AuthorsCowan CW, Shao YR, Sahin M, Shamah SM, Lin MZ, Greer PL, Gao S, Griffith EC, Brugge JS, Greenberg ME
JournalNeuron
PubMed ID15848800
Ephrin signaling through Eph receptor tyrosine kinases can promote attraction or repulsion of axonal growth cones during development. However, the mechanisms that determine whether Eph signaling promotes attraction or repulsion are not known. We show here that the Rho family GEF Vav2 plays a key role in this process. We ... More
Microtubule-targeting drugs rescue axonal swellings in cortical neurons from spastin knockout mice.
Mutations in SPG4, encoding the microtubule-severing protein spastin, are responsible for the most frequent form of hereditary spastic paraplegia (HSP), a heterogeneous group of genetic diseases characterized by degeneration of the corticospinal tracts. We previously reported that mice harboring a deletion in Spg4, generating a premature stop codon, develop progressive ... More
Paired helical filaments from Alzheimer disease brain induce intracellular accumulation of Tau protein in aggresomes.
AuthorsSanta-Maria I, Varghese M, Ksiezak-Reding H, Dzhun A, Wang J, Pasinetti GM,
JournalJ Biol Chem
PubMed ID22496370
Abnormal folding of tau protein leads to the generation of paired helical filaments (PHFs) and neurofibrillary tangles, a key neuropathological feature in Alzheimer disease and tauopathies. A specific anatomical pattern of pathological changes developing in the brain suggests that once tau pathology is initiated it propagates between neighboring neuronal cells, ... More
Visual acuity is reduced in alpha 7 nicotinic receptor knockout mice.
AuthorsOriglia N, Valenzano DR, Moretti M, Gotti C, Domenici L,
JournalInvest Ophthalmol Vis Sci
PubMed ID22281823
Nicotinic receptors (nAChRs) are part of a heterogeneous family of pentameric ligand-gated ion channels that are widely expressed in the visual system. The impact of a7 homomeric nAChRs on visual function was investigated using mutant mice lacking the a7 nicotinic receptor subunit. The spatial resolution limit was measured in a7 ... More
Fluorescence correlation spectroscopy: novel variations of an established technique.
AuthorsHaustein E, Schwille P,
JournalAnnu Rev Biophys Biomol Struct
PubMed ID17477838
Fluorescence correlation spectroscopy (FCS) is one of the major biophysical techniques used for unraveling molecular interactions in vitro and in vivo. It allows minimally invasive study of dynamic processes in biological specimens with extremely high temporal and spatial resolution. By recording and correlating the fluorescence fluctuations of single labeled molecules ... More
Cholera toxin entry into pig enterocytes occurs via a lipid raft- and clathrin-dependent mechanism.
The small intestinal brush border is composed of lipid raft microdomains, but little is known about their role in the mechanism whereby cholera toxin gains entry into the enterocyte. The present work characterized the binding of cholera toxin B subunit (CTB) to the brush border and its internalization. CTB binding ... More
Connectional heterogeneity of the ventral part of the macaque area 46.
AuthorsGerbella M, Borra E, Tonelli S, Rozzi S, Luppino G,
JournalCereb Cortex
PubMed ID22499799
We found that the ventral part of the prefrontal area 46 (46v) is connectionally heterogeneous. Specifically, the rostral part (46vr) displayed an almost exclusive and extensive intraprefrontal connectivity and extraprefrontal connections limited to area 24 and inferotemporal areas. In contrast, the caudal part (46vc) mostly displayed intraprefrontal connectivity with ventrolateral ... More
The zebra finch paradox: song is little changed, but number of neurons doubles.
AuthorsWalton C, Pariser E, Nottebohm F,
JournalJ Neurosci
PubMed ID22262875
New neurons are added to the high vocal center (HVC) of adult males in seasonally breeding songbirds such as the canary (Serinus canaria) that learns new songs in adulthood, and the song sparrow (Melospiza melodia) that does not. In both cases, the new neurons numerically replace others that have died, ... More
Engineering surfaces for substrate-mediated gene delivery using recombinant proteins.
AuthorsRea JC, Gibly RF, Davis NE, Barron AE, Shea LD,
JournalBiomacromolecules
PubMed ID19775146
Immobilized fibronectin and other natural proteins have been utilized to enhance substrate-mediated gene delivery, with apparent contributions from the intrinsic bioactivity and also physical properties of the immobilized proteins. In this report, we investigated the use of recombinant proteins, compared to the full-length fibronectin protein, as surface coatings for gene ... More
Annexin A5 stimulates autophagy and inhibits endocytosis.
AuthorsGhislat G, Aguado C, Knecht E,
JournalJ Cell Sci
PubMed ID22266906
Macroautophagy is a major lysosomal catabolic process activated particularly under starvation in eukaryotic cells. A new organelle, the autophagosome, engulfs cytoplasmic substrates, which are degraded after fusion with endosomes and/or lysosomes. During a shotgun proteome analysis of purified lysosomal membranes from mouse fibroblasts, a Ca(2+)-dependent phospholipid-binding protein, annexin A5, was ... More
Effect of line tension on the lateral organization of lipid membranes.
AuthorsGarcía-Sáez AJ, Chiantia S, Schwille P,
JournalJ Biol Chem
PubMed ID17848582
The principles of organization and functioning of cellular membranes are currently not well understood. The raft hypothesis suggests the existence of domains or rafts in cell membranes, which behave as protein and lipid platforms. They have a functional role in important cellular processes, like protein sorting or cell signaling, among ... More
Clicked bivalent ligands containing curcumin and cholesterol as multifunctional abeta oligomerization inhibitors: design, synthesis, and biological characterization.
In our effort to develop multifunctional compounds that cotarget beta-amyloid oligomers (AbetaOs), cell membrane/lipid rafts (CM/LR), and oxidative stress, a series of bivalent multifunctional Abeta oligomerization inhibitors (BMAOIs) containing cholesterol and curcumin were designed, synthesized, and biologically characterized as potential treatments for Alzheimer's disease (AD). The in vitro assay results ... More
Endocytic trafficking from the small intestinal brush border probed with FM dye.
The small intestinal brush border functions as the body's main portal for uptake of dietary nutrients and simultaneously acts as the largest permeability barrier against pathogens. To enable this, the digestive enzymes of the brush border are organized in lipid raft microdomains stabilized by cross-linking galectins and intelectin, but little ... More
The clathrin-mediated endocytic pathway participates in dsRNA-induced IFN-beta production.
AuthorsItoh K, Watanabe A, Funami K, Seya T, Matsumoto M,
JournalJ Immunol
PubMed ID18832709
TLR3 and cytoplasmic RIG-I-like receptor (RLR) recognize virus-derived dsRNA and induce type I IFN production in a distinct manner. Human TLR3 localizes to the endosomal compartments in myeloid dendritic cells (mDCs), while it localizes to both the cell surface and interior in fibroblasts and epithelial cells. TLR3 signaling arises in ... More
TRPC channels determine human keratinocyte differentiation: new insight into basal cell carcinoma.
AuthorsBeck B, Lehen'kyi V, Roudbaraki M, Flourakis M, Charveron M, Bordat P, Polakowska R, Prevarskaya N, Skryma R,
JournalCell Calcium
PubMed ID17920677
Aberrant keratinocyte differentiation is considered to be a key mechanism in the onset of hyperproliferative dermatological diseases, including basal cell carcinoma (BCC). It is, therefore, vital to understand what drives keratinocytes to develop such pathological phenotypes. The role of calcium in keratinocyte differentiation is uncontested but the mechanisms controlling calcium-induced ... More
Clustering and lateral concentration of raft lipids by the MAL protein.
AuthorsMagal LG, Yaffe Y, Shepshelovich J, Aranda JF, de Marco Mdel C, Gaus K, Alonso MA, Hirschberg K,
JournalMol Biol Cell
PubMed ID19553470
MAL, a compact hydrophobic, four-transmembrane-domain apical protein that copurifies with detergent-resistant membranes is obligatory for the machinery that sorts glycophosphatidylinositol (GPI)-anchored proteins and others to the apical membrane in epithelia. The mechanism of MAL function in lipid-raft-mediated apical sorting is unknown. We report that MAL clusters formed by two independent ... More
Mechanisms of quantum dot nanoparticle cellular uptake.
AuthorsZhang LW, Monteiro-Riviere NA,
JournalToxicol Sci
PubMed ID19414515
Due to the superior photoemission and photostability characteristics, quantum dots (QD) are novel tools in biological and medical applications. However, the toxicity and mechanism of QD uptake are poorly understood. QD nanoparticles with an emission wavelength of 655 nm are ellipsoid in shape and consist of a cadmium/selenide core with ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
The utilization of pathogen-like cellular trafficking by single chain block copolymer.
Amphiphilic triblock copolymer, poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), Pluronic P85, is unexpectedly shown to utilize sophisticated cellular trafficking mechanisms and enter brain microvessel endothelial cells and primary neurons that are poorly penetrable. Though caveolae serve as a primary entry site for the copolymer single chains, in cells devoid of caveolae, the ... More
Subcellular localization of the antidepressant-sensitive norepinephrine transporter.
AuthorsMatthies HJ, Han Q, Shields A, Wright J, Moore JL, Winder DG, Galli A, Blakely RD,
JournalBMC Neurosci
PubMed ID19545450
BACKGROUND: Reuptake of synaptic norepinephrine (NE) via the antidepressant-sensitive NE transporter (NET) supports efficient noradrenergic signaling and presynaptic NE homeostasis. Limited, and somewhat contradictory, information currently describes the axonal transport and localization of NET in neurons. RESULTS: We elucidate NET localization in brain and superior cervical ganglion (SCG) neurons, aided ... More
Protective role of endogenous gangliosides for lysosomal pathology in a cellular model of synucleinopathies.
AuthorsWei J, Fujita M, Nakai M, Waragai M, Sekigawa A, Sugama S, Takenouchi T, Masliah E, Hashimoto M,
JournalAm J Pathol
PubMed ID19349362
Gangliosides may be involved in the pathogenesis of Parkinson's disease and related disorders, although the precise mechanisms governing this involvement remain unknown. In this study, we determined whether changes in endogenous ganglioside levels affect lysosomal pathology in a cellular model of synucleinopathy. For this purpose, dementia with Lewy body-linked P123H ... More
Novel anti-cholesterol monoclonal immunoglobulin G antibodies as probes and potential modulators of membrane raft-dependent immune functions.
AuthorsBíró A, Cervenak L, Balogh A, Lorincz A, Uray K, Horváth A, Romics L, Matkó J, Füst G, László G,
JournalJ Lipid Res
PubMed ID17023738
Natural autoantibodies against cholesterol are present in the sera of all healthy individuals; their function, production, and regulation, however, are still unclear. Here, we managed to produce two monoclonal anti-cholesterol antibodies (ACHAs) by immunizing mice with cholesterol-rich liposomes. The new ACHAs were specific to cholesterol and to some structurally closely ... More
Invariant computations in local cortical networks with balanced excitation and inhibition.
AuthorsMariño J, Schummers J, Lyon DC, Schwabe L, Beck O, Wiesing P, Obermayer K, Sur M
JournalNat Neurosci
PubMed ID15665876
Cortical computations critically involve local neuronal circuits. The computations are often invariant across a cortical area yet are carried out by networks that can vary widely within an area according to its functional architecture. Here we demonstrate a mechanism by which orientation selectivity is computed invariantly in cat primary visual ... More
Crosslinking a lipid raft component triggers liquid ordered-liquid disordered phase separation in model plasma membranes.
The mechanisms by which a cell uses and adapts its functional membrane organization are poorly understood and are the subject of ongoing investigation and discussion. Here, we study one proposed mechanism: the crosslinking of membrane components. In immune cell signaling (and other membrane-associated processes), a small change in the clustering ... More
MHC class II-peptide complexes and APC lipid rafts accumulate at the immunological synapse.
AuthorsHiltbold EM, Poloso NJ, Roche PA
JournalJ Immunol
PubMed ID12538693
Activation of CD4(+) Th cells requires their cognate interaction with APCs bearing specific relevant MHC class II-peptide complexes. This cognate interaction culminates in the formation of an immunological synapse that contains the various proteins and lipids required for efficient T cell activation. We now show that APC lipid raft membrane ... More
Sterol structure determines the separation of phases and the curvature of the liquid-ordered phase in model membranes.
AuthorsBacia K, Schwille P, Kurzchalia T
JournalProc Natl Acad Sci U S A
PubMed ID15722414
The existence of lipid rafts in biological membranes in vivo is still debated. In contrast, the formation of domains in model systems has been well documented. In giant unilamellar vesicles (GUVs) prepared from ternary mixtures of dioleoyl-phosphatidylcholine/sphingomyelin/cholesterol, a clear separation of liquid-disordered and sphingomyelin-enriched, liquid-ordered phases could be observed. This ... More
Axonal bias at a representational border in adult rat somatosensory cortex (S1).
AuthorsSteen PA, Mason M, Pham L, Lefebvre Y, Hickmott PW
JournalJ Comp Neurol
PubMed ID17154268
The cortex is a highly organized structure and this organization is integral to cortical function. However, the circuitry underlying cortical organization is only partially understood, thus limiting our understanding of cortical function. Within the somatosensory cortex, organization is manifest as a map of the body surface. At the level of ... More
Revealing the topography of cellular membrane domains by combined atomic force microscopy/fluorescence imaging.
AuthorsFrankel DJ, Pfeiffer JR, Surviladze Z, Johnson AE, Oliver JM, Wilson BS, Burns AR
JournalBiophys J
PubMed ID16415053
Simultaneous atomic force microscopy (AFM) and confocal fluorescence imaging were used to observe in aqueous buffer the three-dimensional landscape of the inner surface of membrane sheets stripped from fixed tumor mast cells. The AFM images reveal prominent, irregularly shaped raised domains that label with fluorescent markers for both resting and ... More
OxLDL increases endothelial stiffness, force generation, and network formation.
AuthorsByfield FJ, Tikku S, Rothblat GH, Gooch KJ, Levitan I
JournalJ Lipid Res
PubMed ID16418538
This study investigates the effect of oxidatively modified low density lipoprotein (OxLDL) on the biomechanical properties of human aortic endothelial cells (HAECs). We show that treatment with OxLDL results in a 90% decrease in the membrane deformability of HAECs, as determined by micropipette aspiration. Furthermore, aortic endothelial cells freshly isolated ... More
Antigen-stimulated trafficking from the recycling compartment to the plasma membrane in RBL mast cells.
AuthorsNaal RM, Holowka EP, Baird B, Holowka D
JournalTraffic
PubMed ID12656991
Binding of fluorescein isothiocyanate (FITC)-conjugated cholera toxin B subunit to ganglioside GM1 on RBL-2H3 cells at 37 degrees C results in labeling of the plasma membrane as well as a pool of perinuclear intracellular membranes identified as the endosomal recycling compartment. Antigen-mediated activation of IgE receptor signaling causes rapid, sustained ... More
Spatial compartmentalization of tumor necrosis factor (TNF) receptor 1-dependent signaling pathways in human airway smooth muscle cells. Lipid rafts are essential for TNF-alpha-mediated activation of RhoA but dispensable for the activation of the NF-kappaB and MAPK pathways.
AuthorsHunter I, Nixon GF
JournalJ Biol Chem
PubMed ID16982613
Tumor necrosis factor (TNF)-alpha-induced activation of RhoA, mediated by TNF receptor 1 (TNFR1), is a prerequisite step in a pathway that leads to increased 20-kDa light chain of myosin (MLC20) phosphorylation and airway smooth muscle contraction. In this study, we have investigated the proximal events in TNF-alpha-induced RhoA activation. TNFR1 ... More
Gangliosides inhibit flagellin signaling in the absence of an effect on flagellin binding to toll-like receptor 5.
AuthorsWest AP, Dancho BA, Mizel SB
JournalJ Biol Chem
PubMed ID15632166
A recent study (Ogushi, K., Wada, A., Niidome, T., Okuda, T., Llanes, R., Nakayama, M., Nishi, Y., Kurazono, H., Smith, K. D., Aderem, A., Moss, J., and Hirayama, T. (2004) J. Biol. Chem. 279, 12213-12219) concluded that gangliosides serve as co-receptors for flagellin signaling via toll-like receptor 5 (TLR5). In ... More
Non-natural cell surface receptors: synthetic peptides capped with N-cholesterylglycine efficiently deliver proteins into Mammalian cells.
AuthorsMartin SE, Peterson BR
JournalBioconjug Chem
PubMed ID12526694
Protein toxins such as shiga toxin and cholera toxin penetrate into cells by binding small molecule-based cell surface receptors localized to cholesterol and sphingolipid-rich lipid raft subdomains of cellular plasma membranes. Molecular recognition between these toxins and their receptors triggers endocytic protein uptake through endogenous membrane trafficking pathways. We report ... More
Operant conditioning paradigm for juxtacellular recordings in functionally identified cortical neurons during motor execution in head-fixed rats.
AuthorsSantana-Chávez G, Rodriguez-Moreno P, López-Hidalgo M, Olivares-Moreno R, Moreno-López Y, Rojas-Piloni G
JournalJ Neurosci Methods
PubMed ID31669337
'Understanding the configuration of neural circuits and the specific role of distinct cortical neuron types involved in behavior, requires the study of structure-function and connectivity relationships with single cell resolution in awake behaving animals. Despite head-fixed behaving rats have been used for in vivo measuring of neuronal activity, it is ... More
Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease.
Authors
JournalNat Commun
PubMed ID32107381
Adenosine 2A receptor inhibition protects phrenic motor neurons from cell death induced by protein synthesis inhibition.
AuthorsSeven YB, Simon AK, Sajjadi E, Zwick A, Satriotomo I, Mitchell GS
JournalExp Neurol
PubMed ID31629857
'Respiratory motor neuron survival is critical for maintenance of adequate ventilation and airway clearance, preventing dependence to mechanical ventilation and respiratory tract infections. Phrenic motor neurons are highly vulnerable in rodent models of motor neuron disease versus accessory inspiratory motor pools (e.g. intercostals, scalenus). Thus, strategies that promote phrenic motor ... More
Apoptotic Extracellular Vesicles Ameliorate Multiple Myeloma by Restoring Fas-Mediated Apoptosis.
Authors
JournalACS Nano
PubMed ID34506129
Adaptive liquid interfaces induce neuronal differentiation of mesenchymal stem cells through lipid raft assembly.
Authors
JournalNat Commun
PubMed ID35661107
Cross-talks of glycosylphosphatidylinositol biosynthesis with glycosphingolipid biosynthesis and ER-associated degradation.
Authors
JournalNat Commun
PubMed ID32054864
Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine.
Authors
JournalNature
PubMed ID34234346
Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.
Authors
JournalNat Commun
PubMed ID34824246
Postmitotic control of sensory area specification during neocortical development.
Authors
JournalNat Commun
PubMed ID25476200
Friction Mediates Scission of Tubular Membranes Scaffolded by BAR Proteins.
Authors
JournalCell
PubMed ID28648660
A cortical cell ensemble in the posterior parietal cortex controls past experience-dependent memory updating.
Authors
JournalNat Commun
PubMed ID35017495
Neuropathic pain caused by miswiring and abnormal end organ targeting.
Authors
JournalNature
PubMed ID35614217
Interference with SRF expression in skeletal muscles reduces peripheral nerve regeneration in mice.
AuthorsWanner R, Knöll B
JournalSci Rep
PubMed ID32210317
Traumatic injury of peripheral nerves typically also damages nerve surrounding tissue including muscles. Hence, molecular and cellular interactions of neighboring damaged tissues might be decisive for successful axonal regeneration of injured nerves. So far, the contribution of muscles and muscle-derived molecules to peripheral nerve regeneration has only poorly been studied. ... More
Novel Drug Candidates Improve Ganglioside Accumulation and Neural Dysfunction in GM1 Gangliosidosis Models with Autophagy Activation.
AuthorsKajihara R, Numakawa T, Odaka H, Yaginuma Y, Fusaki N, Okumiya T, Furuya H, Inui S, Era T
JournalStem Cell Reports
PubMed ID32302553
GM1 gangliosidosis is a lysosomal storage disease caused by loss of lysosomal ß-galactosidase activity and characterized by progressive neurodegeneration due to massive accumulation of GM1 ganglioside in the brain. Here, we generated induced pluripotent stem cells (iPSCs) derived from patients with GM1 gangliosidosis, and the resultant neurons showed impaired neurotransmitter ... More
Deleterious Effect of NMDA Plus Kainate on the Inner Retinal Cells and Ganglion Cell Projection of the Mouse.
AuthorsCalvo E, Milla-Navarro S, Ortuño-Lizarán I, Gómez-Vicente V, Cuenca N, De la Villa P, Germain F
JournalInt J Mol Sci
PubMed ID32106602
Combined administration of N-Methyl-D-Aspartate (NMDA) and kainic acid (KA) on the inner retina was studied as a model of excitotoxicity. The right eye of C57BL6J mice was injected with 1 µL of PBS containing NMDA 30 mM and KA 10 mM. Only PBS was injected in the left eye. One ... More
The Fusarium mycotoxin, 2-Amino-14,16-dimethyloctadecan-3-ol (AOD) induces vacuolization in HepG2 cells.
The mycotoxin 2-Amino-14,16-dimethyloctadecan-3-ol (AOD) has been isolated from cultures of the fungus Fusarium avenaceum, one of the most prevalent Fusarium species. AOD is an analogue of sphinganine and 1-deoxysphinganine, important intermediates in the de novo biosynthesis of cellular sphingolipids. Here we studied cellular effects of AOD using the human liver ... More
CRMP2 mediates Sema3F-dependent axon pruning and dendritic spine remodeling.
AuthorsZiak J, Weissova R, Jerábková K, Janikova M, Maimon R, Petrasek T, Pukajova B, Kleisnerova M, Wang M, Brill MS, Kasparek P, Zhou X, Alvarez-Bolado G, Sedlacek R, Misgeld T, Stuchlik A, Perlson E, Balastik M
JournalEMBO Rep
PubMed ID31919978
Regulation of axon guidance and pruning of inappropriate synapses by class 3 semaphorins are key to the development of neural circuits. Collapsin response mediator protein 2 (CRMP2) has been shown to regulate axon guidance by mediating semaphorin 3A (Sema3A) signaling; however, nothing is known about its role in synapse pruning. ... More
Hippocampal Ripple Coordinates Retrosplenial Inhibitory Neurons during Slow-Wave Sleep.
AuthorsOpalka AN, Huang WQ, Liu J, Liang H, Wang DV
JournalCell Rep
PubMed ID31940487
The hippocampus and retrosplenial cortex (RSC) play indispensable roles in memory formation, and importantly, a hippocampal oscillation known as ripple is key to consolidation of new memories. However, it remains unclear how the hippocampus and RSC communicate and the role of ripple oscillation in coordinating the activity between these two ... More
Traffic-related air pollutants (TRAP-PM) promote neuronal amyloidogenesis through oxidative damage to lipid rafts.
AuthorsCacciottolo M, Morgan TE, Saffari AA, Shirmohammadi F, Forman HJ, Sioutas C, Finch CE
JournalFree Radic Biol Med
PubMed ID31883973
Traffic-related air pollution particulate matter (TRAP-PM) is associated with increased risk of Alzheimer Disease (AD). Rodent models respond to nano-sized TRAP-PM (nPM) with increased production of amyloid Aß peptides, concurrently with oxidative damage. Because pro-Aß processing of the amyloid precursor protein (APP) occurs on subcellular lipid rafts, we hypothesized that ... More