AuthorsKrasnow MA, Cumberledge S, Manning G, Herzenberg LA, Nolan GP
JournalScience
PubMed ID1898782
'Use of primary culture cells has been limited by the inability to purify most types of cells, particularly cells from early developmental stages. In whole animal cell sorting (WACS), live cells derived from animals harboring a lacZ transgene are purified according to their level of beta-galactosidase expression with a fluorogenic ... More
Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry.
AuthorsGilbert DF, Wilson JC, Nink V, Lynch JW, Osborne GW,
JournalCytometry A
PubMed ID19184990
Flow cytometry is an important drug discovery tool because it permits high-content multiparameter analysis of individual cells. A new method dramatically enhanced screening throughput by multiplexing many discrete fixed cell populations; however, this method is not suited to assays requiring functional cellular responses. HEK293 cells were transfected with unique mutant ... More
UVA-induced apoptosis studied by the new apo/necro-Comet-assay which distinguishes viable, apoptotic and necrotic cells.
AuthorsMorley N, Rapp A, Dittmar H, Salter L, Gould D, Greulich KO, Curnow A,
JournalMutagenesis
PubMed ID16500949
An adaptation of the Comet-assay was developed which enables the discrimination of viable, apoptotic and necrotic single cells by use of the common Annexin-V staining and a dye exclusion test on the cells already embedded in agarose gel on glass slides. Membrane integrity (Ethidium-Homodimer exclusion), cellular esterase activity (Calcein blue-AM) ... More
Early intermediates in HIV-1 envelope glycoprotein-mediated fusion triggered by CD4 and co-receptor complexes.
AuthorsDimitrov AS, Xiao X, Dimitrov DS, Blumenthal R
JournalJ Biol Chem
PubMed ID11397808
An early step in the process of HIV-1 entry into target cells is the activation of its envelope glycoprotein (GP120-GP41) to a fusogenic state upon binding to target cell CD4 and cognate co-receptor. Incubation of human immunodeficiency virus (HIV)-1 Env-expressing cells with an excess of CD4 and co-recepeptor-bearing target cells ... More
RANK ligand-induced elevation of cytosolic Ca2+ accelerates nuclear translocation of nuclear factor kappa B in osteoclasts.
AuthorsKomarova SV, Pilkington MF, Weidema AF, Dixon SJ, Sims SM
JournalJ Biol Chem
PubMed ID12496256
RANK ligand (RANKL) induces activation of NFkappaB, enhancing the formation, resorptive activity, and survival of osteoclasts. Ca(2+) transduces many signaling events, however, it is not known whether the actions of RANKL involve Ca(2+) signaling. We investigated the effects of RANKL on rat osteoclasts using microspectrofluorimetry and patch clamp. RANKL induced ... More
Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments.
Previous bacterial transport studies have utilized fluorophores which have been shown to adversely affect the physiology of stained cells. This research was undertaken to identify alternative fluorescent stains that do not adversely affect the transport or viability of bacteria. Initial work was performed with a groundwater isolate, Comamonas sp. strain ... More