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查看更多产品信息 CyQUANT™ Cell Proliferation Assays - FAQs (C35012, C35013, C35011, C7027, C7026, C35006, C35007)
4 个常见问题解答
可采用PrestoBlue细胞活力染色剂和CyQUANT细胞增殖检测试剂盒。我们还提供神经突生长染色试剂盒（货号A15001）。更多有关我们的神经元细胞健康检测试剂的信息请见此处（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html）。
The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
When CyQUANT NF dye is bound to DNA, the approximate excitation/emission is 480/520 nm. It can be detected using standard GFP or FITC filter settings.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.