CyQUANT™ NF 细胞增殖检测
CyQUANT™ NF 细胞增殖检测
Invitrogen™

CyQUANT™ NF 细胞增殖检测

CyQUANT™ NF 细胞增殖检测提供了在微孔板内对群体内细胞进行定量的快速灵敏方法。CyQUANT™ NF 细胞增殖检测的特点为:•比 MTT 或 AlamarBlue™ 测定试剂盒更灵敏•线性检测范围为每孔100至20,000个细胞了解更多信息
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货号细胞类型数量
C35007NF Cell200 Assays
C35011Direct Cell10 Microplates
C35013Direct Red Cell10块微孔板
C35012Direct Cell100 Microplates
C7026For cells in culture1000 次检测
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 mL
货号 C35007
价格(CNY)
2,902.00
Each
添加至购物车
细胞类型:
NF Cell
数量:
200 Assays
价格(CNY)
2,902.00
Each
添加至购物车
CyQUANT™ NF 细胞增殖检测提供了在微孔板内对群体内细胞进行定量的快速灵敏方法。

CyQUANT™ NF 细胞增殖检测的特点为:

•比 MTT 或 AlamarBlue™ 测定试剂盒更灵敏
•线性检测范围为每孔100至20,000个细胞(96孔微孔板)
•测定试剂盒可以在一小时内完成

快速简单的细胞增殖检测方法
CyQUANT™ NF 细胞增殖测定试剂盒不需要细胞裂解、长时间孵育、放射性标记或从细胞中去除染色剂。CyQUANT™ NF 检测无需进行原始 CyQUANT™ 细胞增殖检测的冻融细胞裂解步骤,代之以一种细胞通透性的 DNA 结合染料和一种质膜通透性的试剂。CyQUANT™ NF 细胞增殖测定试剂盒可以在96孔或384孔微孔板内使用,有两种包装形式:200次测定试剂盒 (C35007),适用于较小样本量,以及1000次测定试剂盒 (C35006),适用于高通量应用。

AlamarBlue™ 是 TREK Diagnostic Systems 公司的注册商标。
仅供科研使用。不可用于诊断程序。
规格
细胞类型NF Cell
检测方法荧光
染料类型其他标记或染料
激发/发射480/520 nm
产品规格384 孔板、96 孔板
数量200 Assays
运输条件室温
适用于(应用)增殖测定试剂盒
适用于(设备)微孔板读数仪, HTS Reader
产品线CyQUANT™
产品类型细胞增殖测定试剂盒
Unit SizeEach
内容与储存
•CyQUANT™ NF dye reagent
•Dye delivery reagent
•5X HBSS buffer (Hank’s balanced salt solution)

It is possible to observe precipitate in HBSS buffer. The precipitate does not have an impact on the use of this kit, nor the results that are gathered from its use.

常见问题解答 (FAQ)

你们提供测定神经元细胞健康的产品吗?

可采用PrestoBlue细胞活力染色剂和CyQUANT细胞增殖检测试剂盒。我们还提供神经突生长染色试剂盒(货号A15001)。更多有关我们的神经元细胞健康检测试剂的信息请见此处(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html)。

What is the difference between the original CyQuant assay and the CyQuant NF assay?

The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.