'BACKGROUND: Microbial biofilms form on central venous catheters and may be associated with systemic infections as well as decreased dialysis efficiency due to catheter thrombosis. The most widely used anticoagulant catheter lock solution in the US is sodium heparin. We have previously shown that sodium heparin in clinically relevant concentrations ... More
'Although action potentials are typically generated in the axon initial segment (AIS), the timing and pattern of action potentials are thought to depend on inward current originating in somatodendritic compartments. Using two-photon imaging, we show that T- and R-type voltage-gated Ca(2+) channels are colocalized with Na(+) channels in the AIS ... More
A novel role for MNTB neuron dendrites in regulating action potential amplitude and cell excitability during repetitive firing.
AuthorsLeão RN, Leão RM, da Costa LF, Rock Levinson S, Walmsley B,
JournalEur J Neurosci
PubMed ID18598256
'Principal cells of the medial nucleus of the trapezoid body (MNTB) are simple round neurons that receive a large excitatory synapse (the calyx of Held) and many small inhibitory synapses on the soma. Strangely, these neurons also possess one or two short tufted dendrites, whose function is unknown. Here we ... More
Fluorescent metal ion indicators based on benzoannelated crown systems: a green fluorescent indicator for intracellular sodium ions.
AuthorsMartin VV, Rothe A, Gee KR
JournalBioorg Med Chem Lett
PubMed ID15780620
The synthesis and metal binding properties of cation-sensitive fluorescent indicators intended for biological applications are described. The increase of the crown ether ring size enhances the affinity for larger cations, but weakens the fluorescent response and selectivity. A compound having a 15-crown-5 chelator directly attached to a 2,7-difluoroxanthenone fluorophore loads ... More
Characterization and application of controllable local chemical changes produced by reagent delivery from a nanopipet.
AuthorsPiper JD, Li C, Lo CJ, Berry R, Korchev Y, Ying L, Klenerman D,
JournalJ Am Chem Soc
PubMed ID18624405
We introduce a versatile method that allows local and repeatable delivery (or depletion) of any water-soluble reagent from a nanopipet in ionic solution to make localized controlled changes in reagent concentration at a surface. In this work, Na(+) or OH(-) ions were dosed from the pipet using pulsed voltage-driven delivery. ... More
Effects of the noradrenergic system in rat white matter exposed to oxygen-glucose deprivation in vitro.
AuthorsNikolaeva MA, Richard S, Mouihate A, Stys PK,
JournalJ Neurosci
PubMed ID19211886
Norepinephrine (NE) is released in excess into the extracellular space during oxygen-glucose deprivation (OGD) in brain, increasing neuronal metabolism and aggravating glutamate excitoxicity. We used isolated rat optic nerve and spinal cord dorsal columns to determine whether the noradrenergic system influences axonal damage in white matter. Tissue was studied electrophysiologically ... More
Properties of the new fluorescent Na+ indicator CoroNa Green: comparison with SBFI and confocal Na+ imaging.
AuthorsMeier SD, Kovalchuk Y, Rose CR
JournalJ Neurosci Methods
PubMed ID16488020
Neuronal activity causes substantial Na+ transients in fine cellular processes such as dendrites and spines. The physiological consequences of such Na+ transients are still largely unknown. High-resolution Na+ imaging is pivotal to study these questions, and, up to now, two-photon imaging with the fluorescent Na+ indicator sodium-binding benzofuran isophthalate (SBFI) ... More
Na+-dependent sources of intra-axonal Ca2+ release in rat optic nerve during in vitro chemical ischemia.
AuthorsNikolaeva MA, Mukherjee B, Stys PK
JournalJ Neurosci
PubMed ID16251444
The contribution of intracellular stores to axonal Ca2+ overload during chemical ischemia in vitro was examined by confocal microscopy. Ca2+ accumulation was measured by fluo-4 dextran (low-affinity dye, KD approximately 4 microM) or by Oregon Green 488 BAPTA-1 dextran (highaffinity dye, KD approximately 450 nM). Axonal Na+ was measured using ... More
Osmolarity-independent electrical cues guide rapid response to injury in zebrafish epidermis.
Authors
JournalElife
PubMed ID33225997
A novel SGLT is expressed in the human kidney.
Authors
JournalEur J Pharmacol
PubMed ID22766068
Quantitative determination of cellular [Na+] by fluorescence lifetime imaging with CoroNaGreen.