Calcium homeostasis of isolated single cortical fibers of rat lens.
AuthorsSrivastava SK, Wang LF, Ansari NH, Bhatnagar A
JournalInvest Ophthalmol Vis Sci
PubMed ID9344353
'PURPOSE: To investigate the calcium homeostasis in single fiber cells isolated from rat ocular lens cortex and to quantify the changes in the concentration of free intracellular calcium [Ca2+]i during the process of disintegrative globulization. METHODS: Individual fiber cells from the cortex of the adult rat lens were isolated by ... More
Kinetic studies of calcium-induced calcium release in cardiac sarcoplasmic reticulum vesicles.
AuthorsSánchez G, Hidalgo C, Donoso P
JournalBiophys J
PubMed ID12668440
'Fast Ca(2+) release kinetics were measured in cardiac sarcoplasmic reticulum vesicles actively loaded with Ca(2+). Release was induced in solutions containing 1.2 mM free ATP and variable free [Ca(2+)] and [Mg(2+)]. Release rate constants (k) were 10-fold higher at pCa 6 than at pCa 5 whereas Ryanodine binding was highest ... More
Associative learning modifies neural representations of odors in the insect brain.
AuthorsFaber T, Joerges J, Menzel R
JournalNat Neurosci
PubMed ID10195183
Recording brain activity in vivo during learning is fundamental to understanding how memories are formed. We used functional calcium imaging to track odor representations in the primary chemosensory center of the honeybee, the antennal lobe, while training animals to discriminate a rewarded odor from an unrewarded one. Our results show ... More
A method for recording intracellular [Ca2+] transients in cardiac myocytes using calcium green-2.
AuthorsSpencer CI, Berlin JR
JournalPflugers Arch
PubMed ID7491285
Calcium green-2 (Ca green) is a non-ratiometric fluorescent Ca2+ indicator with an affinity for Ca2+ (dissociation constant Kd = 3 microM) that is lower than more commonly used indicators such as fura-2 and fluo-3. This low Ca2+ affinity, coupled with a high quantum yield, allows cells to be loaded with ... More
Characterisation of an intracellular Ca2+ pump in Dictyostelium.
AuthorsRooney EK, Gross JD, Satre M
JournalCell Calcium
PubMed ID7712544
Calcium uptake by microsomal membranes from the cellular slime mould Dictyostelium discoideum was measured using Calcium Green-2 as a fluorescent probe of external free Ca2+ concentration. High-affinity Ca2+ uptake was found to be completely inhibited by low concentrations of vanadate, but not by thapsigargin, suggesting that the activity is mediated ... More
Calcium transients in cerebellar granule cell presynaptic terminals.
AuthorsRegehr WG, Atluri PP
JournalBiophys J
PubMed ID7612860
Calcium ions act presynaptically to modulate synaptic strength and to trigger neurotransmitter release. Here we detect stimulus-evoked changes in residual free calcium ([Ca2+]i) in rat cerebellar granule cell presynaptic terminals. Granule cell axons, known as parallel fibers, and their associated boutons, were labeled with several calcium indicators. When parallel fibers ... More