引用和文献 (6)

Invitrogen™

CellMask™ 细胞膜染色剂

Name: CellMask™ 细胞膜染色剂
SKU: C37608
Price: 2550.00 CNY

Invitrogen CellMask™ 细胞膜染色剂能够快速、均匀地标记几乎所有类型细胞的细胞膜。这种染色剂迅速起效，并可通过任何成像仪器采用标准显微镜滤光片组进行检测。

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货号	颜色
C37608	绿色
C10046	深红色
C10045	橙色

3 选项

货号 C37608

价格（CNY)

2,550.00

飞享价

Ends: 31-Dec-2026

3,541.00

共减 991.00 (28%)

Each

颜色:

绿色

价格（CNY)

2,550.00

飞享价

Ends: 31-Dec-2026

3,541.00

共减 991.00 (28%)

Each

Invitrogen CellMask™ 细胞膜染色剂能够快速、均匀地标记几乎所有类型细胞的细胞膜。这种染色剂迅速起效，并可通过任何成像仪器采用标准显微镜滤光片组进行检测。根据细胞类型和实验条件，细胞膜染色将持续 60–90 分钟而不会出现可检测到的内化，为大多数活细胞动态研究提供了足够的时间。该染料可固定，但不可透化。

Invitrogen CellMask™ 细胞膜染色剂能够快速、均匀地标记几乎所有类型细胞的细胞膜。这种染色剂迅速起效，并可通过任何成像仪器采用标准显微镜滤光片组进行检测。根据细胞类型和实验条件，细胞膜染色将持续 60–90 分钟而不会出现可检测到的内化，为大多数活细胞动态研究提供了足够的时间。这种染色剂可固定，但不可透化。

CellMask™ 细胞膜染色剂的特点包括：
• 灵敏度—特异性和快速的细胞膜染色
• 出色的保留力—染色可持续 60–90 分钟
• 可用于常用的显微镜通道，绿色、橙色、深红色和近红外

其他方法的缺点
细胞膜是细胞边界的便利标记物，因此许多探针已用于细胞膜染色。通常使用亲脂性染料；但是，它们会快速内化，导致成像时间窗非常狭窄。荧光标记的凝集素（如麦胚凝集素）也已经被用作细胞膜染料。偶联的凝集素依赖细胞表面的糖进行染色，因此染色不一致，并且不同细胞类型之间存在差异。稳健的细胞膜染色对于包括转位测定和细胞膜动态变化在内的各种应用都非常重要，并且可用作传统及自动化成像和分析中的通用细胞鉴定工具。

CellMask™ 细胞膜染色剂的作用原理
CellMask™ 细胞膜染色剂经设计用于对各种哺乳动物细胞类型的细胞膜进行均匀染色，与 DiI、DiO 以及标记的麦胚凝集素等传统方法相比，其内化较为缓慢。

CellMask™ 细胞膜染色剂为两亲性分子，包含亲脂性基团（用于实现出色的膜加载）以及带负电荷的亲水性染料（用于将探针锚定在细胞膜中）。CellMask™ 细胞膜染色剂在为活细胞成像提供充足机会的同时，染色模式在甲醛固定后仍然可以保持，从而支持其他多参数成像选择。但是，使用 CellMask™ 细胞膜染色后不能进行去垢剂提取，因此不能与需要透化处理的探针一起使用。

仅供科研使用。不可用于诊断程序。

规格

颜色绿色

描述CellMask™ 绿色细胞膜染料

适用于（设备）共聚焦显微镜、荧光显微镜

产品线Cellmask

数量100 μL

容积（公制）100 μL

标签类型荧光染料

产品类型细胞膜染色剂

亚细胞定位细胞膜

Unit SizeEach

内容与储存

在冷冻冰箱(–5° 至 –30°C)中避光储存。

常见问题解答 (FAQ)

我想标记细胞膜，但可选的染料有多种，该如何选择？

如果进行活细胞成像，CellVue和CellMask 质膜染色剂染色最均匀，被细胞内吞的速度最慢。如果想固定和通透细胞（如进行抗体标记），这种产品并不是最佳选择。麦胚凝集素（WGA）偶联物也能标记活细胞，或者甲醛固定的细胞。它们可以在随后的去垢剂（如TritonX-100）通透处理后保存下来。但如果细胞已经进行通透处理，WGA也会标记内部结构。因此，如果细胞已通透，则只能使用靶向质膜蛋白的抗体。亲脂性花青染料（如DiI）会标记活细胞的所有膜结构，而不仅仅是细胞质膜。此网页（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html）的内容可帮助您进行选择。

Can I use CellMask Plasma membrane stains or Alexa Fluor dye labeled wheat germ agglutinin to label the plasma membrane of my paraffin sections?

No. For paraffin sections, there are few options due to the delipidation of the membranes by solvents used in the deparaffinization steps. The only good option is to use an antibody against a plasma membrane protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does the staining last with CellMask Plasma Membrane Stains?

Depending upon the cell type and experimental conditions, the plasma membrane staining will last for 60-90 min without detectable internalization, providing enough time for most live cell dynamic studies. The stain survives fixation, but not permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What dyes are used to make the CellMask stains?

The proprietary fluorescent dyes in the CellMask stains are general cytoplasmic stains. They are not found to bind to any specific cellular component.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (6)

引用和文献

Abstract

BAR scaffolds drive membrane fission by crowding disordered domains.

Authors:Snead WT, Zeno WF, Kago G, Perkins RW, Richter JB, Zhao C, Lafer EM, Stachowiak JC

Journal:J Cell Biol

PubMed ID:30504247

'Cellular membranes are continuously remodeled. The crescent-shaped bin-amphiphysin-rvs (BAR) domains remodel membranes in multiple cellular pathways. Based on studies of isolated BAR domains in vitro, the current paradigm is that BAR domain-containing proteins polymerize into cylindrical scaffolds that stabilize lipid tubules. But in nature, proteins that contain BAR domains often ... More

Altering integrin engagement regulates membrane localization of K

Authors:Sengupta S, Rothenberg KE, Li H, Hoffman BD, Bursac N

Journal:J Cell Sci

PubMed ID:31391240

How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K ... More

Anchoring cortical granules in the cortex ensures trafficking to the plasma membrane for post-fertilization exocytosis.

Authors:Vogt EJ, Tokuhiro K, Guo M, Dale R, Yang G, Shin SW, Movilla MJ, Shroff H, Dean J

Journal:Nat Commun

PubMed ID:31118423

Following fertilization, cortical granules exocytose ovastacin, a metalloendopeptidase that cleaves ZP2 in the zona pellucida surrounding mouse eggs to prevent additional sperm binding. Using high- and super-resolution imaging with ovastacin ... More

Membrane Mechanical Properties Regulate the Effect of Strain on Spontaneous Electrophysiology in Human iPSC-Derived Neurons.

Authors:Bianchi F, Pereno V, George JH, Thompson MS, Ye H

Journal:Neuroscience

PubMed ID:30817953

Peripheral nerves contain neuron fibers vital for movement and sensation and are subject to continuous elongation and compression during everyday movement. At supraphysiological strains conduction blocks occur, resulting in permanent or temporary loss of function. The mechanisms underpinning these alterations in electrophysiological activity remain unclear; however, there is evidence that ... More

APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.

Authors:Uzureau S, Lecordier L, Uzureau P, Hennig D, Graversen JH, Homblé F, Mfutu PE, Oliveira Arcolino F, Ramos AR, La Rovere RM, Luyten T, Vermeersch M, Tebabi P, Dieu M, Cuypers B, Deborggraeve S, Rabant M, Legendre C, Moestrup SK, Levtchenko E, Bultynck G, Erneux C, Pérez-Morga D, Pays E

Journal:Cell Rep

PubMed ID:32187552

The C-terminal variants G1 and G2 of apolipoprotein L1 (APOL1) confer human resistance to the sleeping sickness parasite Trypanosoma rhodesiense, but they also increase the risk of kidney disease. APOL1 and APOL3 are death-promoting proteins that are partially associated with the endoplasmic reticulum and Golgi membranes. We report that in ... More

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