N-acetylcysteine augments the cellular redox changes and cytotoxic activity of internalized mycobacterium bovis in human bladder cancer cells.
AuthorsPook SH, Esuvaranathan K, Mahendran R
JournalJ Urol
PubMed ID12131368
PURPOSE: We determined whether changes in cellular reactive oxygen species correlated with mycobacteria internalization and bladder cancer cell death. MATERIALS AND METHODS: Reactive oxygen species and thiols in RT112 and MGH bladder cancer cells were determined using the fluorescence probes 5-(and 6)-carboxy-2', 7' dichlorodihydrofluorescein diacetate and monobromobimane. Superoxide and nitrite ... More
The sulphonylurea glibenclamide inhibits multidrug resistance protein (MRP1) activity in human lung cancer cells.
AuthorsPayen L, Delugin L, Courtois A, Trinquart Y, Guillouzo A, Fardel O
JournalBr J Pharmacol
PubMed ID11159731
'1. Glibenclamide, a sulphonylurea widely used for the treatment of non-insulin-dependent diabetes mellitus, has been shown to inhibit the activities of various ATP-binding cassette (ABC) transporters. In the present study, its effects towards multidrug resistance protein 1 (MRP1), an ABC efflux pump conferring multidrug resistance and handling organic anions, were ... More
Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae.
'We identified VTA1 in a screen for mutations that result in altered vacuole morphology. Deletion of VTA1 resulted in delayed trafficking of the lipophilic dye FM4-64 to the vacuole and altered vacuolar morphology when cells were exposed to the dye 5-(and 6)-carboxy-2'',7''-dichlorofluorescein diacetate (CDCFDA). Deletion of class E vacuolar protein ... More
Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes.
AuthorsDiaz G, Liu S, Isola R, Diana A, Falchi AM
JournalHistochem Cell Biol
PubMed ID14574587
'Mitochondria are the main source of reactive oxygen species (ROS). The aim of this work was to verify the ROS generation in situ in HeLa cells exposed to prooxidants and antioxidants (menadione, tert-butyl hydroperoxide, antimycin A, vitamin E, N-acetyl-L-cysteine, and butylated hydroxytoluene) using the ROS-sensitive probes 6-carboxy-2'',7''-dichlorodihydrofluorescein diacetate di-acetomethyl ester ... More
The use of fluorescent probes to assess oxidative processes in isolated-perfused rat heart tissue.
AuthorsKehrer JP, Paraidathathu T
JournalFree Radic Res Commun
PubMed ID1505782
'The formation of reactive oxygen species (ROS) in intact heart tissue has been assessed by direct ESR measurements, and indirectly by the formation of characteristic tissue products and the protective effects of various antioxidants. The development of lipid soluble esters of compounds which can be trapped intracellularly after hydrolysis, and ... More
The cell cycle related apoptotic susceptibility to arsenic trioxide is associated with the level of reactive oxygen species.
AuthorsGao F, Yi J, Yuan JQ, Shi GY, Tang XM
JournalCell Res
PubMed ID15040894
'Double staining flow cytometry was performed using 7-amino actinomycin D and 6-carboxy-2'', 7''-dichlorodihydrofluorescein diacetate, to detect the level fluctuation of reactive oxygen species (ROS) during the cell cycle of normal NB4 cells. Our results showed that NB4 cells possessed higher level of ROS in G2/M phase than in G1 and ... More
Mitochondrial production of reactive oxygen species mediate dicumarol-induced cytotoxicity in cancer cells.
'Dicumarol is a naturally occurring anticoagulant derived from coumarin that induces cytotoxicity and oxidative stress in human pancreatic cancer cells (Cullen, J. J., Hinkhouse, M. M., Grady, M., Gaut, A. W., Liu, J., Zhang, Y., Weydert, C. J. D., Domann, F. E., and Oberley, L. W. (2003) Cancer Res. 63, ... More
NAD(P)H oxidase mediates the endothelial barrier dysfunction induced by TNF-alpha.
AuthorsGertzberg N, Neumann P, Rizzo V, Johnson A
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12807699
'We tested the hypothesis that the NAD(P)H oxidase-dependent generation of superoxide anion (O2-*) mediates tumor necrosis factor-alpha (TNF)-induced alterations in the permeability of pulmonary microvessel endothelial monolayers (PMEM). The permeability of PMEM was assessed by the clearance rate of Evans blue-labeled albumin. The NAD(P)H oxidase subcomponents p47phox and p22phox were ... More
Mechanism of clofibrate hepatotoxicity: mitochondrial damage and oxidative stress in hepatocytes.
AuthorsQu B, Li QT, Wong KP, Tan TM, Halliwell B
JournalFree Radic Biol Med
PubMed ID11522451
'Peroxisome proliferators have been found to induce hepatocarcinogenesis in rodents, and may cause mitochondrial damage. Consistent with this, clofibrate increased hepatic mitochondrial oxidative DNA and protein damage in mice. The present investigation aimed to study the mechanism by which this might occur by examining the effect of clofibrate on freshly ... More
Induction of apoptosis in prostate tumor PC-3 cells and inhibition of xenograft prostate tumor growth by the vanilloid capsaicin.
AuthorsSánchez AM, Sánchez MG, Malagarie-Cazenave S, Olea N, Díaz-Laviada I
JournalApoptosis
PubMed ID16374544
'Capsaicin, the pungent ingredient of hot chilli pepper, has been recently shown to induce apoptosis in several cell lines through a not well known mechanism. Here, we investigated the role of the vanilloid capsaicin in the death regulation of the human cancer androgen-resistant cell line PC-3. Capsaicin inhibited the growth ... More
Molecular ordering in HIV-induced apoptosis. Oxidative stress, activation of caspases, and cell survival are regulated by transaldolase.
AuthorsBanki K, Hutter E, Gonchoroff NJ, Perl A
JournalJ Biol Chem
PubMed ID9565623
'Dysregulated apoptosis may underlie the etiology of T cell depletion by human immunodeficiency virus type 1 (HIV-1). We show that HIV-induced apoptosis is preceded by an exponential increase in reactive oxygen intermediates (ROIs) produced in mitochondria. This leads to caspase-3 activation, phosphatidylserine (PS) externalization, and GSH depletion. Since mitochondrial ROI ... More
SOD2 contributes to anti-oxidative capacity in rabbit corneal endothelial cells.
AuthorsLiu C, Ogando D, Bonanno JA,
JournalMol Vis
PubMed ID21976958
'Corneal endothelial cells are rich in mitochondria, a potential source of reactive oxygen species (ROS). ROS have been implicated in endothelial cell loss during aging or in endothelial dystrophies. In this study we examined the anti-oxidative role of mitochondrial superoxide dismutase (SOD2) in corneal endothelial cells. SOD2 expression was examined ... More
The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis.
AuthorsSaiardi A, Caffrey JJ, Snyder SH, Shears SB
JournalJ Biol Chem
PubMed ID10827188
'Saiardi et al. (Saiardi, A., Erdjument-Bromage, H., Snowman, A., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326) previously described the cloning of a kinase from yeast and two kinases from mammals (types 1 and 2), which phosphorylate inositol hexakisphosphate (InsP(6)) to diphosphoinositol pentakisphosphate, a "high energy" candidate ... More
Apelin attenuates oxidative stress in human adipocytes.
'It has been recently recognized that the increased oxidative stress (ROS overproduction) in obese condition is a key contributor to the pathogenesis of obesity-associated metabolic diseases. Apelin is an adipocytokine secreted by adipocytes, and known for its anti-obesity and anti-diabetic properties. In obesity, both oxidative stress and plasma level of ... More
Ammonia-induced production of free radicals in primary cultures of rat astrocytes.
AuthorsMurthy CR, Rama Rao KV, Bai G, Norenberg MD
JournalJ Neurosci Res
PubMed ID11592125
'Elevated levels of ammonia in blood and brain result in derangement of cerebral function. Recently, lipid peroxidation and oxidative stress have been implicated in ammonia neurotoxicity. Because ammonia is primarily detoxified in astrocytes, we postulated that pathophysiological concentrations of ammonia might induce free radical formation in these cells. To test ... More
Comparative study on the antioxidant capacity of wines and other plant-derived beverages.
'Consistent epidemiological data point to a reduced morbidity and mortality from coronary heart disease and atherosclerosis in people consuming plant-derived beverages such as tea or wine. We studied the antioxidant capacity of three red wines (W) and compared it those of tea and herbal "mate" tea infusions. The antioxidant capacity ... More
L-Homocysteine and L-homocystine stereospecifically induce endothelial nitric oxide synthase-dependent lipid peroxidation in endothelial cells.
AuthorsHeydrick SJ, Weiss N, Thomas SR, Cap AP, Pimentel DR, Loscalzo J, Keaney JF
JournalFree Radic Biol Med
PubMed ID14980706
'Atherothrombotic cardiovascular disease associated with hyperhomocysteinemia has been proposed to result, at least in part, from increased vascular oxidative stress. Here we characterize one mechanism by which homocyteine may induce a vascular cell type-specific oxidative stress. Our results show that L-homocysteine at micromolar levels stereospecifically increases lipid peroxidation in cultured ... More
An oxidative stress-mediated death pathway in irradiated human leukemia cells mapped using multilaser flow cytometry.
AuthorsSheng-Tanner X, Bump EA, Hedley DW
JournalRadiat Res
PubMed ID9840183
'OCI/AML-2 acute myeloid leukemia cells were found to undergo apoptosis after treatment with y rays from a 137Cs source. Multilaser flow cytometry techniques using probes for live cell function were used to monitor the biochemical changes that occurred prior to the loss of surface membrane integrity. These showed increases in ... More
Cytotoxicity, ROS-generation activity and radical-scavenging activity of curcumin and related compounds.
AuthorsFujisawa S, Atsumi T, Ishihara M, Kadoma Y
JournalAnticancer Res
PubMed ID15160995
'The cytotoxicity, ROS (reactive oxygen species)-generation activity and radical-scavenging activity of curcumin and related compounds such as eugenol, eugenol orthodimer (bis-eugenol; 3,3'-dimethoxy-5,5'-di-2-propenyl-1,1'-biphenyl-2,2'-diol) and isoeugenol were investigated. Their cytotoxicity against a human submandibular gland adenocarcinoma cell line (HSG) declined in the order curcumin > isoeugenol > bis-eugenol > eugenol. Since the ... More
Reactive oxygen species play no role in the candidacidal activity of the salivary antimicrobial peptide histatin 5.
AuthorsVeerman EC, Nazmi K, Van't Hof W, Bolscher JG, Den Hertog AL, Nieuw Amerongen AV
JournalBiochem J
PubMed ID15109304
'The mechanism of action of antimicrobial peptides is still a matter of debate. The formation of ROS (reactive oxygen species) has been suggested to be the crucial step in the fungicidal mechanism of a number of antimicrobial peptides, including histatin 5 and lactoferrin-derived peptides. In the present study we have ... More
Nerve growth factor protects against aluminum-mediated cell death.
AuthorsOhyashiki T, Satoh E, Okada M, Takadera T, Sahara M
JournalToxicology
PubMed ID12093616
'In the present study, we examined the effect of two salts of aluminum (Al), aluminum maltolate (Almal) and aluminum chloride (AlCl(3)), on the cell viability of PC12 cells in the absence and presence of nerve growth factor (NGF). A 72-h exposure of PC12 cells to Almal (300 microM) resulted in ... More
Calcium-dependent modulation of poly(ADP-ribose) polymerase-1 alters cellular metabolism and DNA repair.
AuthorsBentle MS, Reinicke KE, Bey EA, Spitz DR, Boothman DA
JournalJ Biol Chem
PubMed ID16920718
'After genotoxic stress poly(ADP-ribose) polymerase-1 (PARP-1) can be hyperactivated, causing (ADP-ribosyl)ation of nuclear proteins (including itself), resulting in NAD(+) and ATP depletion and cell death. Mechanisms of PARP-1-mediated cell death and downstream proteolysis remain enigmatic. beta-lapachone (beta-lap) is the first chemotherapeutic agent to elicit a Ca(2+)-mediated cell death by PARP-1 ... More
Role of reactive oxygen species in gallic acid-induced apoptosis.
AuthorsInoue M, Sakaguchi N, Isuzugawa K, Tani H, Ogihara Y
JournalBiol Pharm Bull
PubMed ID11041242
'We earlier demonstrated that gallic acid (3,4,5-trihydroxybenzoic acid) induced apoptosis in promyelocytic leukemia HL-60RG cells, which was inhibited by catalase and intracellular Ca2+ chelator. In this study, we further studied the involvement of reactive oxygen species (ROS) and intracellular Ca2+ in gallic acid-induced apoptosis. The enhancement of intracellular ROS in ... More
Expression profile of cord blood neutrophils and dysregulation of HSPA1A and OLR1 upon challenge by bacterial peptidoglycan.
AuthorsFong ON, Chan KY, Leung KT, Lam HS, Cheung HM, Leung TY, Li K, Ng PC,
Journal
PubMed ID23986550
'In newborn infants, the innate cellular system plays a crucial role in the first line of defense against pathogens. Neutrophils are the most abundant leukocytes, and their response to the commonly encountered nosocomial bacterial (Gram positive) infection in newborns remains largely unclear. In this study, a genome-wide expression array analysis ... More
Deposition of reactive oxygen metabolites onto and within living tumor cells during neutrophil-mediated antibody-dependent cellular cytotoxicity.
AuthorsCao D, Boxer LA, Petty HR
JournalJ Cell Physiol
PubMed ID8344997
'In this study we test the hypothesis that reactive oxygen metabolites are delivered from neutrophils to simultaneously both the cell surface and cytosol of opsonized YAC erythroleukemic target cells. Using 5'' (or 6'') carboxyl-2'',7''-dichlorodihydrofluorescein (H2-CDCF) diacetate as starting material, we synthesized its succinimidyl ester derivative. H2-CDCF-conjugated IgG prepared from the ... More
Agonist-induced calcium and oxidative stress responses in endothelial cells.
AuthorsWilkinson JA, Jacob R
JournalBiochem Soc Trans
PubMed ID14505459
'[Ca(2+)](i) (cytosolic [Ca(2+)]) and OS (oxidative stress) were measured simultaneously in calf pulmonary artery endothelial cells using fura-2 and carboxy-2'',7''-dichlorodihydrofluorescein. ATP stimulated a [Ca(2+)](i) increase that was followed a few seconds later by an increase in OS. Pre-exposure to 5 microM H(2)O(2) potentiated these responses to ATP. Elevating or removing ... More
Estimation of oxidative stress in Saccharomyces cerevisae with fluorescent probes.
AuthorsJakubowski W, Bartosz G
JournalInt J Biochem Cell Biol
PubMed ID9451827
Oxidation of dichlorofluorescin and dihydrofluorescin and transport of their oxidation products were studied in Saccharomyces cerevisiae cells given diacetates of these compounds. It was found that addition of peroxidase significantly accelerates the formation of dichlorofluorescein and fluorescein, indicating that peroxidase activity may be a limiting factor in studies of cellular ... More
Expression-activity profiles of cells transfected with prostaglandin endoperoxide H synthase measured by quantitative fluorescence microscopy.
AuthorsMorita I, Smith WL, DeWitt DL, Schindler M
JournalBiochemistry
PubMed ID7766630
Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate expression and activity of the ... More
Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects.
AuthorsWardman P,
JournalFree Radic Biol Med
PubMed ID17761297
Chemical probes for free radicals in biology are important tools; fluorescence and chemiluminescence offer high detection sensitivity. This article reviews progress in the development of probes for "reactive oxygen and nitrogen" species, emphasizing the caution needed in their use. Reactive species include hydrogen peroxide; hydroxyl, superoxide, and thiyl radicals; carbonate ... More
Single-stranded DNA induces ataxia telangiectasia mutant (ATM)/p53-dependent DNA damage and apoptotic signals.
AuthorsNur-E-Kamal A, Li TK, Zhang A, Qi H, Hars ES, Liu LF
JournalJ Biol Chem
PubMed ID12540848
Single-stranded DNA has been speculated to be the initial signal in the DNA damage signaling pathway. We showed that introduction of single-stranded DNA with diverse sequences into mammalian cells induced DNA damage as well as apoptosis signals. Like DNA damaging agents, single-stranded DNA up-regulated p53 and activated the nuclear kinase ... More
A new zebrafish model produced by TILLING of SOD1-related amyotrophic lateral sclerosis replicates key features of the disease and represents a tool for in vivo therapeutic screening.
AuthorsDa Costa MM, Allen CE, Higginbottom A, Ramesh T, Shaw PJ, McDermott CJ,
Journal
PubMed ID24092880
Mutations in the superoxide dismutase gene (SOD1) are one cause of familial amyotrophic lateral sclerosis [ALS; also known as motor neuron disease (MND)] in humans. ALS is a relentlessly progressive neurodegenerative disease and, to date, there are no neuroprotective therapies with significant impact on the disease course. Current transgenic murine ... More
FUsed in sarcoma is a novel regulator of manganese superoxide dismutase gene transcription.
AuthorsDhar SK, Zhang J, Gal J, Xu Y, Miao L, Lynn BC, Zhu H, Kasarskis EJ, St Clair DK,
Journal
PubMed ID23834335
FUsed in sarcoma (FUS) is a multifunctional DNA/RNA-binding protein that possesses diverse roles, such as RNA splicing, RNA transport, DNA repair, translation, and transcription. The network of enzymes and processes regulated by FUS is far from being fully described. In this study, we have focused on the mechanisms of FUS-regulated ... More
Autophagy, a major clearance route for many long-lived proteins and organelles, has long been implicated in cancer development. Myc is a proto-oncogene often found to be deregulated in many cancers, and thus is an attractive target for design of cancer therapy. Therefore, understanding the relationship between anti-Myc strategies and autophagy ... More
Differential generation of hydrogen peroxide upon exposure to zinc and cadmium in the hyperaccumulating plant species (Sedum alfredii Hance).
AuthorsChao YE, Zhang M, Tian SK, Lu LL, Yang XE,
JournalJ Zhejiang Univ Sci B
PubMed ID18357627
Sedum alfredii Hance has been identified as zinc (Zn) and cadmium (Cd) co-hyperaccumulator. In this paper the relationships of Zn or Cd hyperaccumulation to the generation and the role of H2O2 in Sedum alfredii H. were examined. The results show that Zn and Cd contents in the shoots of Sedum ... More
AKR1C isoforms represent a novel cellular target for jasmonates alongside their mitochondrial-mediated effects.
AuthorsDavies NJ, Hayden RE, Simpson PJ, Birtwistle J, Mayer K, Ride JP, Bunce CM,
JournalCancer Res
PubMed ID19487289
Members of the aldo-keto reductase (AKR) superfamily, particularly the AKR1C subfamily, are emerging as important mediators of the pathology of cancer. Agents that inhibit these enzymes may provide novel agents for either the chemoprevention or treatment of diverse malignancies. Recently, jasmonates, a family of plant stress hormones that bear a ... More
Interleukin-6 counteracts therapy-induced cellular oxidative stress in multiple myeloma by up-regulating manganese superoxide dismutase.
AuthorsBrown CO, Salem K, Wagner BA, Bera S, Singh N, Tiwari A, Choudhury A, Buettner GR, Goel A,
JournalBiochem J
PubMed ID22471522
IL (interleukin)-6, an established growth factor for multiple myeloma cells, induces myeloma therapy resistance, but the resistance mechanisms remain unclear. The present study determines the role of IL-6 in re-establishing intracellular redox homoeostasis in the context of myeloma therapy. IL-6 treatment increased myeloma cell resistance to agents that induce oxidative ... More
In vitro interaction of zeolite fibers with individual cells (macrophages NR8383): measurement of intracellular oxidative burst.
AuthorsHogg BD, Dutta PK, Long JF
JournalAnal Chem
PubMed ID8686924
Inhalation of fibrous minerals such as asbestos and erionite can cause various lung diseases, including cancer. The mechanism by which these fibers induce disease is an area of active research. Interaction of fibers with lung macrophages leads to release of many substances. Among these, reactive oxygen metabolites (which include hydrogen ... More
DNA repair is limiting for haematopoietic stem cells during ageing.
AuthorsNijnik A, Woodbine L, Marchetti C, Dawson S, Lambe T, Liu C, Rodrigues NP, Crockford TL, Cabuy E, Vindigni A, Enver T, Bell JI, Slijepcevic P, Goodnow CC, Jeggo PA, Cornall RJ
JournalNature
PubMed ID17554302
Accumulation of DNA damage leading to adult stem cell exhaustion has been proposed to be a principal mechanism of ageing. Here we address this question by taking advantage of the highly specific role of DNA ligase IV in the repair of DNA double-strand breaks by non-homologous end-joining, and by the ... More
Neuronal glutathione deficiency and age-dependent neurodegeneration in the EAAC1 deficient mouse.
AuthorsAoyama K, Suh SW, Hamby AM, Liu J, Chan WY, Chen Y, Swanson RA
JournalNat Neurosci
PubMed ID16311588
Uptake of the neurotransmitter glutamate is effected primarily by transporters expressed on astrocytes, and downregulation of these transporters leads to seizures and neuronal death. Neurons also express a glutamate transporter, termed excitatory amino acid carrier-1 (EAAC1), but the physiological function of this transporter remains uncertain. Here we report that genetically ... More
CCC1 is a transporter that mediates vacuolar iron storage in yeast.
AuthorsLi L, Chen OS, McVey Ward D, Kaplan J
JournalJ Biol Chem
PubMed ID11390404
The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that endocytosis-defective yeast (Delta end4) can store iron ... More
Multiple classes of yeast mutants are defective in vacuole partitioning yet target vacuole proteins correctly.
AuthorsWang YX, Zhao H, Harding TM, Gomes de Mesquita DS, Woldringh CL, Klionsky DJ, Munn AL, Weisman LS
JournalMol Biol Cell
PubMed ID8885233
In Saccharomyces cerevisiae the vacuoles are partitioned from mother cells to daughter cells in a cell-cycle-coordinated process. The molecular basis of this event remains obscure. To date, few yeast mutants had been identified that are defective in vacuole partitioning (vac), and most such mutants are also defective in vacuole protein ... More
Mitochondrial free radical signal in ceramide-dependent apoptosis: a putative mechanism for neuronal death in Parkinson's disease.
AuthorsFrance-Lanord V, Brugg B, Michel PP, Agid Y, Ruberg M
JournalJ Neurochem
PubMed ID9326290
Activation of the apoptogenic sphingomyelin-dependent signaling pathway in neuronally differentiated PC12 cells with cell-permeant C2-ceramide resulted in a transient and short-lived emission of reactive oxygen species that was maximal 6 h after the beginning of treatment, followed immediately by nuclear translocation of the transcription factor nuclear factor kappaB. The production ... More
Expression of gamma-glutamyl transpeptidase protects ramos B cells from oxidation-induced cell death.
AuthorsKarp DR, Shimooku K, Lipsky PE
JournalJ Biol Chem
PubMed ID11080500
The ectoenzyme, gamma-glutamyl transpeptidase (GGT, EC ) cleaves glutathione (GSH) to facilitate the recapture of cysteine for synthesis of intracellular GSH. The impact of GGT expression on cell survival during oxidative stress was investigated using the human B cell lymphoblastoid cell line, Ramos. Ramos cells did not express surface GGT ... More
The Cdc42p GTPase and its regulators Nrf1p and Scd1p are involved in endocytic trafficking in the fission yeast Schizosaccharomyces pombe.
AuthorsMurray JM, Johnson DI
JournalJ Biol Chem
PubMed ID11042180
Nrf1p was first identified in a screen for negative regulators of the Cdc42p GTPase. Overexpression of Nrf1p resulted in dose-dependent lethality, with cells exhibiting an ellipsoidal morphology and abnormal vacuolar phenotypes including an increase in vacuolar fusion. Green fluorescent protein (GFP)-Cdc42p and GFP-Nrf1p colocalized to vacuolar membranes and GFP-Nrf1p vacuolar ... More
Fluorescence imaging of reactive oxygen metabolites generated in single macrophage cells (NR8383) upon phagocytosis of natural zeolite (erionite) fibers.
AuthorsLong JF, Dutta PK, Hogg BD
JournalEnviron Health Perspect
PubMed ID9294716
In this paper we address the phenomenon of reactive oxygen metabolite generation subsequent to phagocytosis of mineral fibers by macrophages. Natural erionite fibers were chosen because of their established toxicity. Macrophages (cell line NR8383) were loaded with the dye 5-(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate and exposed to erionite particles by centrifuging cells ... More
Different intracellular locations for prostaglandin endoperoxide H synthase-1 and -2.
AuthorsMorita I, Schindler M, Regier MK, Otto JC, Hori T, DeWitt DL, Smith WL
JournalJ Biol Chem
PubMed ID7738031
The subcellular locations of prostaglandin endoperoxide synthase-1 and -2 (PGHS-1 and -2) were determined by quantitative confocal fluorescence imaging microscopy in murine 3T3 cells and human and bovine endothelial cells using immunocytofluorescence with isozyme-specific antibodies. In all of the cell types examined, PGHS-1 immunoreactivity was found equally distributed in the ... More
Multidrug resistance protein (MRP) activity in normal mature leukocytes and CD34-positive hematopoietic cells from peripheral blood.
Multidrug resistance proteins (MRPs) such as MRP1, MRP2 and MRP3 are membrane efflux pumps involved in multidrug resistance and handling organic anions. In the present study, MRP activity was investigated in normal mature leucocytes and CD34-positive hematopoietic cells from peripheral blood using the flow cytometric carboxy-2',7'-dichlorofluorescein (CF) efflux assay. Basal ... More
Organelle redox of CF and CFTR-corrected airway epithelia.
In cystic fibrosis reduced CFTR function may alter redox properties of airway epithelial cells. Redox-sensitive GFP (roGFP1) and imaging microscopy were used to measure the redox potentials of the cytosol, endoplasmic reticulum (ER), mitochondria, and cell surface of cystic fibrosis nasal epithelial cells and CFTR-corrected cells. We also measured glutathione ... More
Cell cycle-coupled variation in topoisomerase IIalpha mRNA is regulated by the 3'-untranslated region. Possible role of redox-sensitive protein binding in mRNA accumulation.
AuthorsGoswami PC, Sheren J, Albee LD, Parsian A, Sim JE, Ridnour LA, Higashikubo R, Gius D, Hunt CR, Spitz DR
JournalJ Biol Chem
PubMed ID10986283
Mammalian topoisomerase IIalpha (Topo II) is a highly regulated enzyme essential for many cellular processes including the G(2) cell cycle checkpoint. Because Topo II gene expression is regulated posttranscriptionally during the cell cycle, we investigated the possible role of the 3'-untranslated region (3'-UTR) in controlling Topo II mRNA accumulation. Reporter ... More
Glucose deprivation-induced cytotoxicity and alterations in mitogen-activated protein kinase activation are mediated by oxidative stress in multidrug-resistant human breast carcinoma cells.
AuthorsLee YJ, Galoforo SS, Berns CM, Chen JC, Davis BH, Sim JE, Corry PM, Spitz DR
JournalJ Biol Chem
PubMed ID9478987
We previously observed that glucose deprivation induces cell death in multidrug-resistant human breast carcinoma cells (MCF-7/ADR). As a follow up we wished to test the hypothesis that metabolic oxidative stress was the causative process or at least the link between causative processes behind the cytotoxicity. In the studies described here, ... More
Nuclear translocation of NF-kappaB is increased in dopaminergic neurons of patients with parkinson disease.
AuthorsHunot S, Brugg B, Ricard D, Michel PP, Muriel MP, Ruberg M, Faucheux BA, Agid Y, Hirsch EC
JournalProc Natl Acad Sci U S A
PubMed ID9207126
Evidence from postmortem studies suggest an involvement of oxidative stress in the degeneration of dopaminergic neurons in Parkinson disease (PD) that have recently been shown to die by apoptosis, but the relationship between oxidative stress and apoptosis has not yet been elucidated. Activation of the transcription factor NF-kappaB is associated ... More
Synchronized whole cell oscillations in mitochondrial metabolism triggered by a local release of reactive oxygen species in cardiac myocytes.
AuthorsAon MA, Cortassa S, Marbán E, O'Rourke B
JournalJ Biol Chem
PubMed ID12930841
Reactive oxygen species (ROS) and/or Ca2+ overload can trigger depolarization of mitochondrial inner membrane potential (DeltaPsim) and cell injury. Little is known about how loss of DeltaPsim in a small number of mitochondria might influence the overall function of the cell. Here we employ the narrow focal excitation volume of ... More
Endothelial NADPH oxidase: mechanism of activation by low-density lipoprotein.
AuthorsO'Donnell RW, Johnson DK, Ziegler LM, DiMattina AJ, Stone RI, Holland JA
JournalEndothelium
PubMed ID14741844
Exposure to atherogenic levels of low-density lipoprotein (LDL) causes elevated reactive oxygen species (ROS) production by human endothelial cells (ECs). NADPH oxidase is thought to be the main source of ROS generated by LDL-activated ECs. The mechanism by which this lipoprotein activates endothelial NADPH oxidase is incompletely understood. To gain ... More
Palmitate-induced apoptosis can occur through a ceramide-independent pathway.
AuthorsListenberger LL, Ory DS, Schaffer JE
JournalJ Biol Chem
PubMed ID11278654
Cytotoxic accumulation of long chain fatty acids has been proposed to play an important role in the pathogenesis of diabetes mellitus and heart disease. To explore the mechanism of cellular lipotoxicity, we cultured Chinese hamster ovary cells in the presence of media supplemented with fatty acid. The saturated fatty acid ... More
Zinc status of human IMR-32 neuroblastoma cells influences their susceptibility to iron-induced oxidative stress.
AuthorsMackenzie GG, Keen CL, Oteiza PI
JournalDev Neurosci
PubMed ID12401950
The current work tested the hypothesis that the zinc status of a cell influences its sensitivity to iron-induced oxidative stress. Human IMR-32 neuroblastoma cells were cultured for 24 h in nonchelated control media (5 microM zinc; 4.5 microM iron), or in media that was treated with DTPA to reduce its ... More
Ceramide prevents motoneuronal cell death through inhibition of oxidative signal.
AuthorsIrie F, Hirabayashi Y
JournalNeurosci Res
PubMed ID10616917
We previously reported that cell death of rat spinal motoneurons, induced by trophic factor-deprivation, was attenuated by the application of exogenous cell-permeable ceramide (C6-Cer), or bacterial sphingomyelinase (SMase). Recently, motoneuronal cell death was demonstrated to be mediated by the generation of reactive oxygen species (ROS), including superoxide and peroxinitrite. In ... More
Direct oxidation of 2',7'-dichlorodihydrofluorescein by pyocyanin and other redox-active compounds independent of reactive oxygen species production.
AuthorsO'Malley YQ, Reszka KJ, Britigan BE
JournalFree Radic Biol Med
PubMed ID14732293
Formation of dichlorofluorescein (DCF), the fluorescent oxidation product of 2',7'-dichlorodihydrofluorescein (DCFH2), in cells loaded with the latter compound is often used to detect ROS formation. We previously found that exposure of DCFH2-loaded A549 cells to the Pseudomonas aeruginosa secretory product pyocyanin results in DCF formation, consistent with ROS production. However, ... More
Increased production of reactive oxygen species in hyperglycemic conditions requires dynamic change of mitochondrial morphology.
AuthorsYu T, Robotham JL, Yoon Y,
JournalProc Natl Acad Sci U S A
PubMed ID16477035
Increased production of mitochondrial reactive oxygen species (ROS) by hyperglycemia is recognized as a major cause of the clinical complications associated with diabetes and obesity [Brownlee, M. (2001) Nature 414, 813-820]. We observed that dynamic changes in mitochondrial morphology are associated with high glucose-induced overproduction of ROS. Mitochondria undergo rapid ... More
Characterization and inhibition by a wide range of xenobiotics of organic anion excretion by primary human hepatocytes.
AuthorsPayen L, Courtois A, Campion JP, Guillouzo A, Fardel O
JournalBiochem Pharmacol
PubMed ID11108814
Organic anion secretion by human hepatocytes was characterized using primary liver parenchymal cell cultures and the anionic fluorescent dye carboxy-2',7'-dichlorofluorescein (CF). Probenecid, a well-known common blocker of the membrane transport process for anions, was shown to increase CF accumulation in primary human hepatocytes by inhibiting cellular CF efflux in a ... More
Cellular carbonyl stress enhances the expression of plasminogen activator inhibitor-1 in rat white adipocytes via reactive oxygen species-dependent pathway.
AuthorsUchida Y, Ohba K, Yoshioka T, Irie K, Muraki T, Maru Y
JournalJ Biol Chem
PubMed ID14610081
Carbonyl stress is one of the important mechanisms of tissue damage in vascular complications of diabetes. In the present study, we observed that the plasminogen activator inhibitor-1 (PAI-1) levels in serum and its gene expression in adipose tissue were up-regulated in aged OLETF rats, model animals of obese type 2 ... More
Characterization of the potent neuroprotective properties of the natural vitamin E alpha-tocotrienol.
AuthorsKhanna S, Roy S, Parinandi NL, Maurer M, Sen CK
JournalJ Neurochem
PubMed ID16923160
The natural vitamin E tocotrienols possess properties not shared by tocopherols. Nanomolar alpha-tocotrienol, not alpha-tocopherol, is potently neuroprotective. On a concentration basis, this finding represents the most potent of all biological functions exhibited by any natural vitamin E molecule. We sought to dissect the antioxidant-independent and -dependent neuroprotective properties of ... More
Simultaneous detection of mitochondrial respiratory chain activity and reactive oxygen in digitonin-permeabilized cells using flow cytometry.
AuthorsPham NA, Robinson BH, Hedley DW
JournalCytometry
PubMed ID11084609
BACKGROUND: Increased mitochondrial generation of reactive oxygen intermediates (ROI) due to defective respiratory chain activity has been implicated in physiological processes such as apoptosis, in the pathogenesis of mitochondrial diseases, and as part of the normal aging process. Established methods addressing activity of the respiratory chain complexes have been limited ... More
Diesel exhaust particle-induced cell death of cultured normal human bronchial epithelial cells.
AuthorsMatsuo M, Shimada T, Uenishi R, Sasaki N, Sagai M
JournalBiol Pharm Bull
PubMed ID12673022
We investigated the effect of diesel exhaust particles (DEPs) on normal human bronchial epithelial (NHBE) cells. Inclusion of DEPs in culture media was lethal to NHBE cells. NHBE cells are more susceptible to DEPs than other normal human lung cells, normal human pulmonary artery endothelial cells and normal human embryonic ... More
Screening assays for the mitochondrial permeability transition using a fluorescence multiwell plate reader.
AuthorsBlattner JR, He L, Lemasters JJ
JournalAnal Biochem
PubMed ID11488625
Opening of permeability transition (PT) pores in the mitochondrial inner membrane causes the mitochondrial permeability transition (MPT) and leads to mitochondrial swelling, membrane depolarization, and release of intramitochondrial solutes. Here, our aim was to develop high-throughput assays using a fluorescence plate reader to screen potential inducers and blockers of the ... More
Enhanced sensitivity of insulin-resistant adipocytes to vanadate is associated with oxidative stress and decreased reduction of vanadate (+5) to vanadyl (+4).
AuthorsLu B, Ennis D, Lai R, Bogdanovic E, Nikolov R, Salamon L, Fantus C, Le-Tien H, Fantus IG
JournalJ Biol Chem
PubMed ID11463798
Vanadate (sodium orthovanadate), an inhibitor of phosphotyrosine phosphatases (PTPs), mimics many of the metabolic actions of insulin in vitro and in vivo. The potential of vanadate to stimulate glucose transport independent of the early steps in insulin signaling prompted us to test its effectiveness in an in vitro model of ... More
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123.
AuthorsHempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM
JournalFree Radic Biol Med
PubMed ID10443931
To detect intracellular oxidant formation during reoxygenation of anoxic endothelium, the oxidant-sensing fluorescent probes, 2',7'-dichlorodihydrofluorescein diacetate, dihydrorhodamine 123, or 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate were added to human umbilical vein endothelial cells during reoxygenation. None of these fluorescent probes were able to differentiate the controls from the reoxygenated cells in the confocal ... More
Free radical-induced megamitochondria formation and apoptosis.
AuthorsKarbowski M, Kurono C, Wozniak M, Ostrowski M, Teranishi M, Nishizawa Y, Usukura J, Soji T, Wakabayashi T
JournalFree Radic Biol Med
PubMed ID9895232
Pathophysiological meaning and the mechanism of the formation of megamitochondria (MG) induced under physiological and pathological conditions remain obscure. We now provide evidence suggesting that the MG formation may be a prerequisite for free radical-mediated apoptosis. MG were detected in primary cultured rat hepatocytes, rat liver cell lines RL-34 and ... More
Cholesterol-rich plasma membrane domains (lipid rafts) in keratinocytes: importance in the baseline and UVA-induced generation of reactive oxygen species.
AuthorsGniadecki R, Christoffersen N, Wulf HC
JournalJ Invest Dermatol
PubMed ID11918702
The biologic effects of ultraviolet radiation such as DNA damage, mutagenesis, cellular aging, and carcinogenesis are in part mediated by reactive oxygen species. In unirradiated cells the major known sources of reactive oxygen species are the mitochondrial respiratory chain and the membrane oxidases functionally coupled to several membrane growth factor ... More
Persistent activation of ERK contributes to glutamate-induced oxidative toxicity in a neuronal cell line and primary cortical neuron cultures.
AuthorsStanciu M, Wang Y, Kentor R, Burke N, Watkins S, Kress G, Reynolds I, Klann E, Angiolieri MR, Johnson JW, DeFranco DB
JournalJ Biol Chem
PubMed ID10766856
Oxidative stress can trigger neuronal cell death and has been implicated in several chronic neurological diseases and in acute neurological injury. Oxidative toxicity can be induced by glutamate treatment in cells that lack ionotrophic glutamate receptors, such as the immortalized HT22 hippocampal cell line and immature primary cortical neurons. Previously, ... More
Phenobarbital alters hepatic Mrp2 function by direct and indirect interactions.
Phenobarbital (PB) treatment impairs the biliary excretion of some organic anions. One mechanism may involve direct competition for biliary excretion by PB and/or a PB metabolite. Alternatively, PB may alter the expression and/or function of hepatic organic anion transport proteins. The role of multidrug resistance-associated protein 2 (Mrp2) in the ... More
Cycloheximide and 4-OH-TEMPO suppress chloramphenicol-induced apoptosis in RL-34 cells via the suppression of the formation of megamitochondria.
AuthorsKarbowski M, Kurono C, Wozniak M, Ostrowski M, Teranishi M, Soji T, Wakabayashi T
JournalBiochim Biophys Acta
PubMed ID10076048
Toxic effects of chloramphenicol, an antibiotic inhibitor of mitochondrial protein synthesis, on rat liver derived RL-34 cell line were completely blocked by a combined treatment with substances endowed with direct or indirect antioxidant properties. A stable, nitroxide free radical scavenger, 4-hydroxy-2,2,6, 6-tetramethylpiperidine-1-oxyl, and a protein synthesis inhibitor, cycloheximide, suppressed in ... More
Antioxidants N-acetylcysteine (NAC) and 2-mercaptoethanol (2-ME) affect the survival and differentiative potential of cholinergic precursors from the embryonic septal nuclei and basal forebrain: involvement of ras signaling.
AuthorsNi L, Wen Y, Peng X, Jonakait GM
JournalBrain Res Dev Brain Res
PubMed ID11675123
We investigated the effects of antioxidants N-acetylcysteine (NAC) and 2-mercaptoethanol (2-ME) on the expression of choline acetyltransferase (ChAT) in cultured cholinergic precursors from the embryonic rat septal nuclei and basal forebrain. Carboxy-dichlorofluorescein fluorescence confirmed that 2-ME inhibited intracellular oxidation. Low micromolar concentrations of 2-ME produce as much as a 12-fold ... More
Optical imaging reveals elevated intracellular chloride in hippocampal pyramidal neurons after oxidative stress.
AuthorsSah R, Schwartz-Bloom RD
JournalJ Neurosci
PubMed ID10531424
The accumulation of reactive oxygen species (ROS) in the brain is associated with several neurodegenerative conditions. ROS can affect ionic homeostasis leading to impaired neurotransmission. Here, we determined the ability of H(2)O(2), a membrane permeant ROS, to alter intraneuronal Cl(-), an important regulator of neuronal excitability. Real-time alterations in intracellular ... More
Transaldolase is essential for maintenance of the mitochondrial transmembrane potential and fertility of spermatozoa.
AuthorsPerl A, Qian Y, Chohan KR, Shirley CR, Amidon W, Banerjee S, Middleton FA, Conkrite KL, Barcza M, Gonchoroff N, Suarez SS, Banki K
JournalProc Natl Acad Sci U S A
PubMed ID17003133
Fertility of spermatozoa depends on maintenance of the mitochondrial transmembrane potential (Deltapsi(m)), which is generated by the electron-transport chain and regulated by an oxidation-reduction equilibrium of reactive oxygen intermediates, pyridine nucleotides, and glutathione (GSH). Here, we report that male mice lacking transaldolase (TAL)(-/-) are sterile because of defective forward motility. ... More
Cytosolic phospholipase A2 (PLA2), but not secretory PLA2, potentiates hydrogen peroxide cytotoxicity in kidney epithelial cells.
AuthorsSapirstein A, Spech RA, Witzgall R, Bonventre JV
JournalJ Biol Chem
PubMed ID8702935
Phospholipase A2 (PLA2) and reactive oxygen species have been implicated both individually and synergistically in various forms of cellular injury. The form(s) of PLA2 important for cell injury and the implications of enhanced activity of the enzyme, however, have not been discerned. Previous studies reveal an increase in PLA2 activity ... More
Heat shock and the activation of AP-1 and inhibition of NF-kappa B DNA-binding activity: possible role of intracellular redox status.
AuthorsMattson D, Bradbury CM, Bisht KS, Curry HA, Spitz DR, Gius D
JournalInt J Hyperthermia
PubMed ID15195516
The early response genes comprising the AP-1 and NF-kappa B transcription factors are induced by environmental stress and thought to modulate responses to injury processes through the induction of target genes. Exposure to heat and ionizing radiation (IR) has been shown to affect signalling machinery involved in AP-1 and NF-kappa ... More
Thalidomide modulates nuclear redox status and preferentially depletes glutathione in rabbit limb versus rat limb.
AuthorsHansen JM, Harris KK, Philbert MA, Harris C
JournalJ Pharmacol Exp Ther
PubMed ID11861780
Thalidomide produces numerous birth defects, the most notable being phocomelia. Mechanisms behind thalidomide-induced malformations have not been fully elucidated, although recent evidence suggests a role for reactive oxygen species. A thalidomide-resistant (rat) and -sensitive (rabbit) species were used to compare potential inherent differences related to oxidative stress that may provide ... More
Sulforaphane-induced cell death in human prostate cancer cells is initiated by reactive oxygen species.
AuthorsSingh SV, Srivastava SK, Choi S, Lew KL, Antosiewicz J, Xiao D, Zeng Y, Watkins SC, Johnson CS, Trump DL, Lee YJ, Xiao H, Herman-Antosiewicz A
JournalJ Biol Chem
PubMed ID15764812
We have shown previously that sulforaphane (SFN), a constituent of many edible cruciferous vegetables including broccoli, suppresses growth of prostate cancer cells in culture as well as in vivo by causing apoptosis, but the sequence of events leading to cell death is poorly defined. Using PC-3 and DU145 human prostate ... More
Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells.
AuthorsLai YL, Lin CY, Jiang WC, Ho YC, Chen CH, Yet SF
JournalRedox Biol
PubMed ID29216542
'Heme oxygenase (HO)-1 is an inducible stress response protein and well known to protect cells and tissues against injury. Despite its important function in cytoprotection against physiological stress, the role of HO-1 in embryonic stem cell (ESC) differentiation remains largely unknown. We showed previously that induced pluripotent stem (iPS) cells ... More
High Content Analysis of Human Pluripotent Stem Cell Derived Hepatocytes Reveals Drug Induced Steatosis and Phospholipidosis.
AuthorsPradip A, Steel D, Jacobsson S, Holmgren G, Ingelman-Sundberg M, Sartipy P, Björquist P, Johansson I, Edsbagge J
JournalStem Cells Int
PubMed ID26880940
'Hepatotoxicity is one of the most cited reasons for withdrawal of approved drugs from the market. The use of nonclinically relevant in vitro and in vivo testing systems contributes to the high attrition rates. Recent advances in differentiating human induced pluripotent stem cells (hiPSCs) into pure cultures of hepatocyte-like cells ... More
Phenotypic and functional changes in peripheral blood monocytes during progression of human immunodeficiency virus infection. Effects of soluble immune complexes, cytokines, subcellular particulates from apoptotic cells, and HIV-1-encoded proteins on monocytes phagocytic function, oxidative burst, transendothelial migration, and cell surface phenotype.
Authors
JournalJ Clin Invest
PubMed ID7706478
The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.
Authors
JournalCell Stem Cell
PubMed ID30930145
STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient.
Authors
JournalRedox Biol
PubMed ID29078168
Low-dose radiation exposure induces a HIF-1-mediated adaptive and protective metabolic response.
Authors
JournalCell Death Differ
PubMed ID24583639
Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut.
Authors
JournalCell Host Microbe
PubMed ID22817988
Infection regulates pro-resolving mediators that lower antibiotic requirements.
Authors
JournalNature
PubMed ID22538616
Autophagy maintains the metabolism and function of young and old stem cells.
Authors
JournalNature
PubMed ID28241143
A mechanically-induced colon cancer cell population shows increased metastatic potential.
Authors
JournalMol Cancer
PubMed ID24884630
Cytoplasmic condensation induced by membrane damage is associated with antibiotic lethality.
Authors
JournalNat Commun
PubMed ID33875652
Evaluation of the cytotoxicity of the Bithionol - cisplatin combination in a panel of human ovarian cancer cell lines.
AuthorsAyyagari VN, Hsieh TJ, Diaz-Sylvester PL, Brard L
JournalBMC Cancer
PubMed ID28086831
Combination drug therapy appears a promising approach to overcome drug resistance and reduce drug-related toxicities in ovarian cancer treatments. In this in vitro study, we evaluated the antitumor efficacy of cisplatin in combination with Bithionol (BT) against a panel of ovarian cancer cell lines with special focus on cisplatin-sensitive and ... More
Bacterial persistence induced by salicylate via reactive oxygen species.
AuthorsWang T, El Meouche I, Dunlop MJ
JournalSci Rep
PubMed ID28281556
Persisters are phenotypic variants of regular cells that exist in a dormant state with low metabolic activity, allowing them to exhibit high tolerance to antibiotics. Despite increasing recognition of their role in chronic and recalcitrant infections, the mechanisms that induce persister formation are not fully understood. In this study, we ... More
Epigallocatechin-3-O-gallate alleviates the malignant phenotype in A-431 epidermoid and SK-BR-3 breast cancer cell lines.
AuthorsFilippi A, Picot T, Aanei CM, Nagy P, Szöllosi J, Campos L, Ganea C, Mocanu MM
JournalInt J Food Sci Nutr
PubMed ID29157036
In this study, we evaluated the effects of epigallocatechin-3-O-gallate (EGCG) in two cancer cell lines, A-431 overexpressing ErbB1 and SK-BR-3, overexpressing ErbB2. EGCG treatment showed dose-dependent collapse of mitochondrial membrane potential (?? ... More
Effect of High Glucose-Induced Oxidative Stress on Paraoxonase 2 Expression and Activity in Caco-2 Cells.
AuthorsMorresi C, Cianfruglia L, Sartini D, Cecati M, Fumarola S, Emanuelli M, Armeni T, Ferretti G, Bacchetti T
JournalCells
PubMed ID31835890
(1) Background: Hyperglycemia leads to several biochemical and physiological consequences, such as the generation of advanced glycation end products (AGEs) and reactive oxygen species (ROS), which are involved in the development of several human diseases. Intestinal cells are continuously exposed to pro-oxidants and lipid peroxidation products from ingested foods, and ... More
Assessment of acyl-CoA cholesterol acyltransferase (ACAT-1) role in ovarian cancer progression-An in vitro study.
AuthorsAyyagari VN, Wang X, Diaz-Sylvester PL, Groesch K, Brard L
JournalPLoS One
PubMed ID31978092
Abnormal accumulation of acyl-CoA cholesterol acyltransferase-1 (ACAT-1) mediated cholesterol ester has been shown to contribute to cancer progression in various cancers including leukemia, glioma, breast, pancreatic and prostate cancers. However, the significance of ACAT-1 and cholesterol esters (CE) is relatively understudied in ovarian cancer. In this in vitro study, we ... More
Suppression of antibiotic resistance evolution by single-gene deletion.
AuthorsHorinouchi T, Maeda T, Kotani H, Furusawa C
JournalSci Rep
PubMed ID32144279
Antibiotic treatment generally results in the selection of resistant bacterial strains, and the dynamics of resistance evolution is dependent on complex interactions between cellular components. To better characterize the mechanisms of antibiotic resistance and evaluate its dependence on gene regulatory networks, we performed systematic laboratory evolution of Escherichia coli strains ... More