Characterization of a Novel Drosophila melanogaster Galectin. EXPRESSION IN DEVELOPING IMMUNE, NEURAL, AND MUSCLE TISSUES.
Authors Pace Karen E; Lebestky Tim; Hummel Thomas; Arnoux Pascal; Kwan Kent; Baum Linda G;
JournalJ Biol Chem
PubMed ID11809773
'We have cloned and characterized the first galectin to be identified in Drosophila melanogaster. The amino acid sequence of Drosophila galectin showed striking sequence similarity to invertebrate and vertebrate galectins and contained amino acids that are crucial for binding beta-galactoside sugars. Confirming its identity as a galectin family member, the ... More
A gene encoding a protein modified by the phytohormone indoleacetic acid.
Authors Walz Alexander; Park Seijin; Slovin Janet P; Ludwig-Müller Jutta; Momonoki Yoshie S; Cohen Jerry D;
JournalProc Natl Acad Sci U S A
PubMed ID11830675
'We show that the expression of an indole-3-acetic acid (IAA)-modified protein from bean seed, IAP1, is correlated to the developmental period of rapid growth during seed development. Moreover, this protein undergoes rapid degradation during germination. The gene for IAP1, the most abundant protein covalently modified by IAA (iap1, GenBank accession ... More
Overexpression, purification, and site-directed spin labeling of the Nramp metal transporter from Mycobacterium leprae.
Authors Reeve Ian; Hummell David; Nelson Nathan; Voss John;
JournalProc Natl Acad Sci U S A
PubMed ID12077319
'It has long been recognized that the pathogenicity of a broad range of intracellular parasites depends on the availability of transition metal ions, especially iron. Nramp1 (natural resistance-associated macrophage protein 1), a proton-coupled divalent metal ion transporter, has been identified as a controlling factor in the resistance or susceptibility to ... More
Identification of the catalytic residues of alpha-amino acid ester hydrolase from Acetobacter turbidans by labeling and site-directed mutagenesis.
Authors Polderman-Tijmes Jolanda J; Jekel Peter A; Jeronimus-Stratingh C Margot; Bruins Andries P; Van Der Laan Jan-Metske; Sonke Theo; Janssen Dick B;
JournalJ Biol Chem
PubMed ID12011065
'The alpha-amino acid ester hydrolase from Acetobacter turbidans ATCC 9325 is capable of hydrolyzing and synthesizing the side chain peptide bond in beta-lactam antibiotics. Data base searches revealed that the enzyme contains an active site serine consensus sequence Gly-X-Ser-Tyr-X-Gly that is also found in X-prolyl dipeptidyl aminopeptidase. The serine hydrolase ... More
Arginine 343 and 350 are two active residues involved in substrate binding by human Type I D-myo-inositol 1,4,5,-trisphosphate 5- phosphatase.
AuthorsCommuni D, Lecocq R, Erneux C
JournalJ Biol Chem
PubMed ID8662625
'The crucial role of two reactive arginyl residues within the substrate binding domain of human Type I D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) 5-phosphatase has been investigated by chemical modification and site-directed mutagenesis. Chemical modification of the enzyme by phenylglyoxal is accompanied by irreversible inhibition of enzymic activity. Our studies demonstrate that phenylglyoxal ... More
The COOH Terminus of Arylamine N-Acetyltransferase from Salmonella typhimurium Controls Enzymic Activity.
Authors Mushtaq Adeel; Payton Mark; Sim Edith;
JournalJ Biol Chem
PubMed ID11799105
Arylamine N-acetyltransferases (NATs) are a homologous family of enzymes, which acetylate arylamines, arylhydroxylamines, and arylhydrazines by acetyl transfer from acetyl-coenzyme A (Ac-CoA) and are found in many organisms. NAT was first identified as the enzyme responsible for the inactivation of the anti-tubercular drug isoniazid in humans. The three-dimensional structure of ... More
Endogenous heparan sulfate and heparin modulate bone morphogenetic protein-4 signaling and activity.
AuthorsKhan SA, Nelson MS, Pan C, Gaffney PM, Gupta P,
JournalAm J Physiol Cell Physiol
PubMed ID18385288
Bone morphogenetic proteins (BMPs) and their endogenous antagonists are important for brain and bone development and tumor initiation and progression. Heparan sulfate (HS) proteoglycans (HSPG) modulate the activities of BMPs and their antagonists. How glycosaminoglycans (GAGs) influence BMP activity in various malignancies and in inherited abnormalities of GAG metabolism, and ... More
Identification of Sequence Motifs Responsible for the Adhesive Interaction between Exon v10-containing CD44 Isoforms.
Authors Hayes Gregory M.; Chiu Roland; Carpenito Carmine; Dougherty Shona T.; Dougherty Graeme J.;
JournalJ Biol Chem
PubMed ID12407110
Previous studies have demonstrated that CD44 isoforms containing the alternatively spliced exon v10 promote cell-cell adhesion via a mechanism that involves the recognition of chondroitin sulfate side chains presented on the surface of interacting cells in association with other CD44 molecules. Sequence analysis revealed the presence within exon v10 of ... More
Infectivity-associated changes in the transcriptional repertoire of the malaria parasite sporozoite stage.
Authors Matuschewski Kai; Ross Jessica; Brown Stuart M; Kaiser Karine; Nussenzweig Victor; Kappe Stefan H I;
JournalJ Biol Chem
PubMed ID12177071
Injection of Plasmodium salivary gland sporozoites into the vertebrate host by Anopheles mosquitoes initiates malaria infection. Sporozoites develop within oocysts in the mosquito midgut and then enter and mature in the salivary glands. Although morphologically similar, oocyst sporozoites and salivary gland sporozoites differ strikingly in their infectivity to the mammalian ... More
Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009.
Authors Pootoolal Jeff; Thomas Michael G; Marshall C Gary; Neu John M; Hubbard Brian K; Walsh Christopher T; Wright Gerard D;
JournalProc Natl Acad Sci U S A
PubMed ID12060705
The glycopeptide antibiotics vancomycin and teicoplanin are vital components of modern anti-infective chemotherapy exhibiting outstanding activity against Gram-positive pathogens including members of the genera Streptococcus, Staphylococcus, and Enterococcus. These antibiotics also provide fascinating examples of the chemical and associated biosynthetic complexity exploitable in the synthesis of natural products by actinomycetes ... More
Complex regulation of human neuronal nitric-oxide synthase exon 1c gene transcription. Essential role of Sp and ZNF family members of transcription factors.
Neuronal nitric-oxide synthase (nNOS) is expressed in a variety of human tissues and shows a complex transcriptional regulation with the presence of nine alternative first exons (1a-1i) resulting in nNOS transcripts with differing 5'-untranslated regions. We previously demonstrated that nNOS exon 1c, one of the predominant transcripts in the human ... More
Characterization of a thermophilic P-type Ag+/Cu+-ATPase from the extremophile Archaeoglobus fulgidus.
The thermophilic, sulfur metabolizing Archaeoglobus fulgidus contains two genes, AF0473 and AF0152, encoding for PIB-type heavy metal transport ATPases. In this study, we describe the cloning, heterologous expression, purification, and functional characterization of one of these ATPases, CopA (NCB accession number AAB90763), encoded by AF0473. CopA is active at high ... More