Cloning and Characterization of a Cryptic Haloacid Dehalogenase from Burkholderia cepacia MBA4
AuthorsJ. Tsang and L. Sam
JournalJ Bacteriol
PubMed ID10498712
Burkholderia cepacia MBA4 has been shown to produce a single dehalogenase batch culture.Moreover, other cryptic dehalogenases were also detected when the cells were grown in continuousculture. In this paper, we report the cloning and characterization of one of the cryptic dehalogenases inMBA4. This cryptic haloacid dehalogenase, designated Chd1, was expressed ... More
Endogenous heparan sulfate and heparin modulate bone morphogenetic protein-4 signaling and activity.
AuthorsKhan SA, Nelson MS, Pan C, Gaffney PM, Gupta P,
JournalAm J Physiol Cell Physiol
PubMed ID18385288
Bone morphogenetic proteins (BMPs) and their endogenous antagonists are important for brain and bone development and tumor initiation and progression. Heparan sulfate (HS) proteoglycans (HSPG) modulate the activities of BMPs and their antagonists. How glycosaminoglycans (GAGs) influence BMP activity in various malignancies and in inherited abnormalities of GAG metabolism, and ... More
Methyl-CpG binding domain protein 2 represses transcription from hypermethylated pi-class glutathione S-transferase gene promoters in hepatocellular carcinoma cells.
Authors Bakker Jila; Lin Xiaohui; Nelson William G;
JournalJ Biol Chem
PubMed ID11960994
During the pathogenesis of human hepatocellular carcinoma (HCC), the CpG island encompassing the pi-class glutathione S-transferase gene (GSTP1) becomes hypermethylated. Repression of transcription accompanying CpG island hypermethylation has been proposed to be mediated by methyl-CpG binding domain (MBD) proteins. We report here that inhibition of transcription from hypermethylated GSTP1 promoters ... More