Subcellular targeting of RGS9-2 is controlled by multiple molecular determinants on its membrane anchor, R7BP.
AuthorsSong JH, Waataja JJ, Martemyanov KA,
JournalJ Biol Chem
PubMed ID16574655
'RGS9-2, a member of the R7 RGS protein family of neuronal RGS (Regulators of G protein Signaling), is a critical regulator of G protein signaling. In striatal neurons, RGS9-2 is tightly associated with a novel palmitoylated protein - R7BP (R7 family Binding Protein). Here we report that R7BP acts to ... More
A single cell density-sensing factor stimulates distinct signal transduction pathways through two different receptors.
Authors Deery William J; Gao Tong; Ammann Robin; Gomer Richard H;
JournalJ Biol Chem
PubMed ID12070170
'In Dictyostelium discoideum, cell density is monitored by levels of a secreted protein, conditioned medium factor (CMF). CMFR1 is a putative CMF receptor necessary for CMF-induced G protein-independent accumulation of the SP70 prespore protein but not for CMF-induced G protein-dependent inositol 1,4,5-trisphosphate production. Using recombinant fragments of CMF, we find ... More
Tight Binding Inhibition of Protein Phosphatase-1 by Phosphatidic Acid. SPECIFICITY OF INHIBITION BY THE PHOSPHOLIPID.
Authors Jones Jeffrey A; Hannun Yusuf A;
JournalJ Biol Chem
PubMed ID11856740
'Phosphatidic acid (PA) has been identified as a bioactive lipid second messenger, yet despite extensive investigation, no cellular target has emerged as a mediator of its described biological effects. In this study, we identify the gamma isoform of the human protein phosphatase-1 catalytic subunit (PP1cgamma) as a high affinity in ... More
HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes [published erratum appears in Development 1997 Sep;134(17):3218]
AuthorsZhu D, Dix DJ, Eddy EM
JournalDevelopment
PubMed ID9247342
'Cyclin B-dependent CDC2 kinase activity has a key role in triggering the G2/M-phase transition during the mitotic and meiotic cell cycles. The Hsp70-2 gene is expressed only in spermatogenic cells at a significant level. In Hsp70-2 gene knock-out (Hsp70-2(-/-)) mice, primary spermatocytes fail to complete meiosis I, suggesting a link ... More
Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called ... More
Site-directed mutagenesis of large (13-kb) plasmids in a single-PCR procedure
AuthorsWang J, Wilkinson MF
JournalBiotechniques
PubMed ID11084857
Although there are many methods for site-specific mutagenesis, most are only applicable for short (<7 kb) plasmids. The few methods that have been developed for longer plasmids are complicated procedures that involve multiple PCR steps or complx recombination and ligation reactions. Here, we provide a simple single-PCR ... More
Physical interaction between the herpes simplex virus 1 origin-binding protein and single-stranded DNA-binding protein ICP8.
AuthorsBoehmer PE, Lehman IR
JournalProc Natl Acad Sci U S A
PubMed ID8397405
We had previously demonstrated that the herpes simplex virus 1 (HSV-1) single-stranded DNA-binding protein (ICP8) can specifically stimulate the helicase activity of the HSV-1 origin-binding protein (UL9). We show here that this functional stimulation is a manifestation of a tight interaction between UL9 protein and ICP8. By using protein- affinity ... More
Endogenous heparan sulfate and heparin modulate bone morphogenetic protein-4 signaling and activity.
AuthorsKhan SA, Nelson MS, Pan C, Gaffney PM, Gupta P,
JournalAm J Physiol Cell Physiol
PubMed ID18385288
Bone morphogenetic proteins (BMPs) and their endogenous antagonists are important for brain and bone development and tumor initiation and progression. Heparan sulfate (HS) proteoglycans (HSPG) modulate the activities of BMPs and their antagonists. How glycosaminoglycans (GAGs) influence BMP activity in various malignancies and in inherited abnormalities of GAG metabolism, and ... More
The chloroplast protein import receptors Toc34 and Toc159 are phosphorylated by distinct protein kinases.
Authors Fulgosi Hrvoje; Soll Jurgen;
JournalJ Biol Chem
PubMed ID11773075
The molecular composition of chloroplast outer and inner envelope translocons is fairly well established, but little is known about mechanisms and elements involved in import regulation. After synthesis in the cytosol, chloroplast targeted precursor proteins are recognized by outer envelope receptors Toc34 and Toc159. Phosphorylation plays an important role in ... More
Targeting and functional role of N-RAP, a nebulin-related LIM protein, during myofibril assembly in cultured chick cardiomyocytes.
Authors Carroll S L; Herrera A H; Horowits R;
JournalJ Cell Sci
PubMed ID11739655
Targeting and functional effects of N-RAP domains were studied by expression as GFP-tagged fusion proteins in cultured embryonic chick cardiomyocytes. GFP-tagged N-RAP was targeted to myofibril precursors, myofibril ends and cell contacts, expression patterns that are similar to endogenous N-RAP. The GFP-tagged N-RAP LIM domain (GFP-N-RAP-LIM) was targeted to the ... More
Methyl-CpG binding domain protein 2 represses transcription from hypermethylated pi-class glutathione S-transferase gene promoters in hepatocellular carcinoma cells.
Authors Bakker Jila; Lin Xiaohui; Nelson William G;
JournalJ Biol Chem
PubMed ID11960994
During the pathogenesis of human hepatocellular carcinoma (HCC), the CpG island encompassing the pi-class glutathione S-transferase gene (GSTP1) becomes hypermethylated. Repression of transcription accompanying CpG island hypermethylation has been proposed to be mediated by methyl-CpG binding domain (MBD) proteins. We report here that inhibition of transcription from hypermethylated GSTP1 promoters ... More
Characterization of monomeric L1 metallo-beta -lactamase and the role of the N-terminal extension in negative cooperativity and antibiotic hydrolysis.
Authors Simm Alan M; Higgins Catherine S; Carenbauer Anne L; Crowder Michael W; Bateson John H; Bennett Peter M; Clarke Anthony R; Halford Stephen E; Walsh Timothy R;
JournalJ Biol Chem
PubMed ID11940588
The L1 metallo-beta-lactamase from Stenotrophomonas maltophilia is unique among this class of enzymes because it is tetrameric. Previous work predicted that the two regions of important intersubunit interaction were the residue Met-140 and the N-terminal extensions of each subunit. The N-terminal extension was also implicated in beta-lactam binding. Mutation of ... More