Systematic cloning of Treponema pallidum open reading frames for protein expression and antigen discovery.
AuthorsMcKevitt M, Patel K, Smajs D, Marsh M, McLoughlin M, Norris SJ, Weinstock GM, Palzkill T,
JournalGenome Res
PubMed ID12805273
'A topoisomerase-based method was used to clone PCR products encoding 991 of the 1041 open reading frames identified in the genome sequence of the bacterium that causes syphilis, Treponema pallidum subsp. pallidum. Cloning the open reading frames into the univector plasmid system permitted the rapid conversion of the original clone ... More
Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol.
AuthorsTakata K, Shimizu T, Iwai S, Wood RD,
JournalJ Biol Chem
PubMed ID16787914
'Human DNA polymerase N (POLN or pol nu) is the most recently discovered nuclear DNA polymerase in the human genome. It is an A-family DNA polymerase related to Escherichia coli pol I, human POLQ, and Drosophila Mus308. We report the first purification of the recombinant enzyme and examination of its ... More
ATP stimulates signal recognition particle (SRP)/FtsY-supported protein integration in chloroplasts.
Authors Yuan Jianguo; Kight Alicia; Goforth Robyn L; Moore Misty; Peterson Eric C; Sakon Joshua; Henry Ralph;
JournalJ Biol Chem
PubMed ID12105232
'The signal recognition particle (SRP) and its receptor (FtsY in prokaryotes) are essential for cotranslational protein targeting to the endoplasmic reticulum in eukaryotes and the cytoplasmic membrane in prokaryotes. An SRP/FtsY-like protein targeting/integration pathway in chloroplasts mediates the posttranslational integration of the light-harvesting chlorophyll a/b-binding protein (LHCP) into thylakoid membranes. ... More
Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system.
AuthorsStudier FW
JournalJ Mol Biol
PubMed ID2023259
'Bacteriophage T7 lysozyme, a natural inhibitor of T7 RNA polymerase, can reduce basal activity from an inducible gene for T7 RNA polymerase and allow relatively toxic genes to be established in the same cell under control of a T7 promoter. Low levels of T7 lysozyme supplied by plasmids pLysS or ... More
Structural mechanism for the carriage and release of thyroxine in the blood.
AuthorsZhou A, Wei Z, Read RJ, Carrell RW,
JournalProc Natl Acad Sci U S A
PubMed ID16938877
'The hormones that most directly control tissue activities in health and disease are delivered by two noninhibitory members of the serpin family of protease inhibitors, thyroxine-binding globulin (TBG) and corticosteroid-binding globulin. The structure of TBG bound to tetra-iodo thyroxine, solved here at 2.8 A, shows how the thyroxine is carried ... More
Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.
'A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of histone H3 (H3-K4) and potentiates transcription activation. The histone H3 tail interacts specifically with the histone deacetylase NuRD complex. Methylation ... More
A novel hematopoietic granulin induces proliferation of goldfish (Carassius auratus L.) macrophages.
AuthorsHanington PC, Barreda DR, Belosevic M,
JournalJ Biol Chem
PubMed ID16473876
'Granulins are a group of highly conserved growth factors that have been described from a variety of organisms spanning the metazoa. In this study, goldfish granulin was one of the most commonly identified transcripts in the differential cross-screening of macrophage cDNA libraries and was preferentially expressed in proliferating macrophages. Unlike ... More
Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called ... More
Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity.
AuthorsAbramovitch RB, Janjusevic R, Stebbins CE, Martin GB,
JournalProc Natl Acad Sci U S A
PubMed ID16477026
Microbial pathogens of both plants and animals employ virulence factors that suppress the host immune response. The tomato pathogen Pseudomonas syringae injects the AvrPtoB type III effector protein into the plant cell to suppress programmed cell death (PCD) associated with plant immunity. AvrPtoB also inhibits PCD in yeast, indicating that ... More
Solution structure of the catalytic domain of GCN5 histone acetyltransferase bound to coenzymeA
AuthorsLin, Y., Fletcher, M., Zhou, J., Allis, C.D. and Wagner, G.
JournalNature
PubMed ID10403255
Gene transcription requires the release of inactive DNA from its packaging of histone proteins. Following the discovery of the first transcription-associated histone acetyltransferase, tetrahymena GCN5, it was shown that yeast GCN5 is recruited to the promoter and causes hyper-acetylation of histones and transcriptional activation of target genes, establishing a direct ... More