Pointed-end capping by tropomodulin3 negatively regulates endothelial cell motility.
AuthorsFischer RS, Fritz-Six KL, Fowler VM
JournalJ Cell Biol
PubMed ID12707310
'Actin filament pointed-end dynamics are thought to play a critical role in cell motility, yet regulation of this process remains poorly understood. We describe here a previously uncharacterized tropomodulin (Tmod) isoform, Tmod3, which is widely expressed in human tissues and is present in human microvascular endothelial cells (HMEC-1). Tmod3 is ... More
Phallotoxin and actin binding assay by fluorescence enhancement.
AuthorsHuang ZJ, Haugland RP, You WM, Haugland RP
JournalAnal Biochem
PubMed ID1595896
The fluorescence of five fluorophores conjugated to phallotoxins was found to be specifically enhanced upon binding to F-actin in a polymerizing buffer. Rhodamine phalloidin had the greatest fluorescence enhancement of ninefold. The fluorescence titration of rhodamine phalloidin by actin was shown to be consistent with stoichiometric binding. The fluorescence enhancement ... More
The binding of fluorescent phallotoxins to actin in myofibrils.
AuthorsSzczesna D, Lehrer SS
JournalJ Muscle Res Cell Motil
PubMed ID8126219
Fluorescence microscope observation of myofibrils incubated with rhodamine-phalloidin and coumarine-phallacidin showed an initial appearance of fluorescence bands at the Z-lines and near the middle of the sarcomeres indicating preferential binding of dye to actin subunits located at both actin filament ends. After long incubation times (1-3 h) however, a final ... More
Inhibition of focal adhesion kinase (FAK) signaling in focal adhesions decreases cell motility and proliferation.
AuthorsGilmore AP, Romer LH
JournalMol Biol Cell
PubMed ID8856665
It has been proposed that the focal adhesion kinase (FAK) mediates focal adhesion formation through tyrosine phosphorylation during cell adhesion. We investigated the role of FAK in focal adhesion structure and function. Loading cells with a glutathione-S-transferase fusion protein (GST-Cterm) containing the FAK focal adhesion targeting sequence, but not the ... More