A multipurpose vector system for the screening of libraries in bacteria, insect
and mammalian cells and expression in vivo.
AuthorsLaitinen OH, Airenne KJ, Hytönen VP, Peltomaa E, Mähönen AJ, Wirth T, Lind MM, Mäkelä KA, Toivanen PI, Schenkwein D, Heikura T, Nordlund HR, Kulomaa MS, Ylä-Herttuala S,
JournalNucleic Acids Res
PubMed ID15731335
We have constructed a novel tetra-promoter vector (pBVboostFG) system that
enables screening of gene/cDNA libraries for functional genomic studies. The vector enables an all-in-one
strategy for gene expression in mammalian, bacterial and insect cells and is also suitable for direct
use in vivo. Virus preparation is based on an improved mini Tn7 transpositional ... More
Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde.
AuthorsTieman D, Taylor M, Schauer N, Fernie AR, Hanson AD, Klee HJ,
JournalProc Natl Acad Sci U S A
PubMed ID16698923
'An important phenylalanine-derived volatile compound produced by plants is 2-phenylethanol. It is a major contributor to flavor in many foods, including fresh fruits, such as tomato, and an insect-attracting scent in roses and many other flowers. Despite the centrality of 2-phenylethanol to flavor and fragrance, the plant genes responsible for ... More
Identification of an antiangiogenic FGF2-binding site in the N terminus of the soluble pattern recognition receptor PTX3.
AuthorsCamozzi M, Rusnati M, Bugatti A, Bottazzi B, Mantovani A, Bastone A, Inforzato A, Vincenti S, Bracci L, Mastroianni D, Presta M,
JournalJ Biol Chem
PubMed ID16769728
'Long-pentraxin 3 (PTX3) is a soluble pattern recognition receptor with non-redundant functions in inflammation and innate immunity. PTX3 comprises a pentraxin-like C-terminal domain involved in complement activation via C1q interaction and an N-terminal extension with unknown functions. PTX3 binds fibroblast growth factor-2 (FGF2), inhibiting its pro-angiogenic and pro-restenotic activity. Here, ... More
Domain structure and DNA binding regions of beta protein from bacteriophage lambda.
beta protein from bacteriophage lambda promotes a single-strand annealing reaction that is central to Red-mediated recombination at double-strand DNA breaks and chromosomal ends. beta protein binds most tightly to an intermediate of annealing formed by the sequential addition of two complementary oligonucleotides. Here we have characterized the domain structure of ... More
Proteolytic processing of sapovirus ORF1 polyprotein.
The genome of Sapovirus (SaV), a causative agent of gastroenteritis in humans and swine, contains either two or three open reading frames (ORFs). Functional motifs characteristic to the 2C-like NTPase (NTPase), VPg, 3C-like protease (Pro), 3D-like RNA-dependent RNA polymerase (Pol), and capsid protein (VP1) are encoded in the ORF1 polyprotein, ... More
Cytosolic Hydroxymethyldihydropterin Pyrophosphokinase/Dihydropteroate Synthase from Arabidopsis thaliana: A SPECIFIC ROLE IN EARLY DEVELOPMENT AND STRESS RESPONSE.
AuthorsStorozhenko S, Navarrete O, Ravanel S, De Brouwer V, Chaerle P, Zhang GF, Bastien O, Lambert W, Rébeillé F, Van Der Straeten D,
JournalJ Biol Chem
PubMed ID17289662
In plants, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase (mitHPPK/DHPS) is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis. Mining the Arabidopsis genome data base has revealed a second gene encoding a protein that lacks a potential transit peptide, suggesting a cytosolic localization of the isoenzyme (cytHPPK/DHPS). When ... More
Structural mechanics of the pH-dependent activity of beta-carbonic anhydrase from Mycobacterium tuberculosis.
AuthorsCovarrubias AS, Bergfors T, Jones TA, Högbom M,
JournalJ Biol Chem
PubMed ID16321983
Carbonic anhydrases catalyze the reversible hydration of carbon dioxide to form bicarbonate, a reaction required for many functions, including carbon assimilation and pH homeostasis. Carbonic anhydrases are divided into at least three classes and are believed to share a zinc-hydroxide mechanism for carbon dioxide hydration. beta-carbonic anhydrases are broadly spread ... More
PAIR2 is essential for homologous chromosome synapsis in rice meiosis I.
AuthorsNonomura K, Nakano M, Eiguchi M, Suzuki T, Kurata N,
JournalJ Cell Sci
PubMed ID16410547
The PAIR2 gene is required for homologous chromosome synapsis at meiosis I in rice (Oryza sativa L.) and encodes a HORMA-domain protein that is homologous to Saccharomyces cerevisiae HOP1 and Arabidopsis ASY1. Immunocytological and electron microscopic analyses indicate that PAIR2 proteins associate with axial elements (AEs) at leptotene and zygotene, ... More
Serinc, an activity-regulated protein family, incorporates serine into membrane lipid synthesis.
AuthorsInuzuka M, Hayakawa M, Ingi T,
JournalJ Biol Chem
PubMed ID16120614
Cell membranes contain various transporter proteins, some of which are responsible for transferring amino acids across membrane. In this study, we report another class of carrier proteins, termed Serinc1-5, that incorporates a polar amino acid serine into membranes and facilitates the synthesis of two serine-derived lipids, phosphatidylserine and sphingolipids. Serinc ... More