Injection of ultrasmall samples and single molecules into tapered capillaries.
AuthorsChiu DT, Hsiao A, Gaggar A, Garza-López RA, Orwar O, Zare RN
JournalAnal Chem
PubMed ID9164157
A novel injection scheme is described in which ultrasmall samples in the attoliter (10(-18) L) and low femtoliter (10(-15) L) range, or even single molecules, are controllably introduced into a tapered capillary so that electrophoretic separation can be carried out. To match the dimensions of the capillary inlet with that ... More
Applications of fluorescence correlation spectroscopy: measurement of size-mass relationship of native and denatured schizophyllan.
AuthorsLeng X, Starchev K, Buffle J
JournalBiopolymers
PubMed ID11473353
Diffusion dynamics of a polysaccharide, schizophyllan has been studied by fluorescence correlation spectroscopy (FCS). Several different sizes of nondenatured and denatured schizophyllan have been labeled with rhodamine 6G in borate buffer. The length of the nondenatured schizophyllan was calculated from FCS data by using the Broersma's relationship for rod-like macromolecules. ... More