A 'puff and advance' technique for visually controlled staining of turtle retinal ganglion cells.
Authors:Ammermüller J, Guiloff G, Normann R, Kolb H
Journal:J Neurosci Methods
PubMed ID:1696675
We describe a 'puff and advance' technique for visually controlled staining of retinal ganglion cells (GCs) in the unfixed, living retina for light and electron microscopy. Glass microelectrodes are filled with rhodamine-isothiocyanate labeled horseradish peroxidase (Rh-HRP), or Lucifer yellow (LY), or a mixture of both, or with 5,6-carboxytetramethylrhodamine (5,6-Rh) and ... More