I'm making a yeast genomic library and want to transform and amplify it in one of your competent cell strains. What genotype features should I look for in choosing a good strain?
We would recommend a mcr/mrr- strain, which prevents restriction of methylated eukaryotic DNA in the E. coli host. We would also recommend using a T1R strain, as T1 is a common contaminant in genomic/cDNA libraries.