Calcium Green™-1 葡聚糖、钾盐,3000 MW,阴离子
Calcium Green™-1 葡聚糖、钾盐,3000 MW,阴离子
Invitrogen™

Calcium Green™-1 葡聚糖、钾盐,3000 MW,阴离子

标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。其可用于多种钙信号转导研究,包括测量细胞内 Ca2+、跟踪 Ca2+ 进入和释放以及对活组织中 Ca2+ 进行多光子激发成像了解更多信息
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货号数量
C6765
又称 C-6765
5 mg
货号 C6765
又称 C-6765
价格(CNY)
6,791.00
飞享价
Ends: 31-Dec-2025
8,907.00
共减 2,116.00 (24%)
Each
添加至购物车
数量:
5 mg
价格(CNY)
6,791.00
飞享价
Ends: 31-Dec-2025
8,907.00
共减 2,116.00 (24%)
Each
添加至购物车
标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。其可用于多种钙信号转导研究,包括测量细胞内 Ca2+、跟踪 Ca2+ 进入和释放以及对活组织中 Ca2+ 进行多光子激发成像。可以使用膜片移液管、显微注射或我们的 Influx 胞饮上样试剂,以物理方式将细胞不可透过性盐形式的这些葡聚糖偶联指示剂上样至细胞中。利用荧光显微镜检查测量这些细胞的荧光信号。与 AM 酯形式相比,我们的葡聚糖形式的钙指示剂显示泄漏和区室化出现显著下降。

了解有关钙离子、钾离子、pH 和膜电位指示剂等离子指示剂更多信息 ›

钙指示剂(细胞不可透过性盐,葡聚糖偶联物)规格:
• 标记(激发/发射波长):Calcium Green™ -1 (506/531 nm)
•结合 Ca2+ 后荧光强度增加:∼14 倍
•葡聚糖规格:3,000 MW
• 结合 Ca2+ 后显示荧光增强,波长变化较小


Molecular Probes 的光谱特性™ 钙指示剂
这些探针被可见光激发,并且由于激发所需能量低,细胞光损伤电位降低。常用的激光仪器(如共聚焦激光扫描显微镜)能够有效激发这类指示剂,且其发射光谱位于通常不受细胞自发荧光和散射背景干扰的光谱区域。

更丰富的钙荧光指示剂的选择
我们提供了大量 Molecular Probes™ 钙指示剂,可用于各种实验方案,例如右旋糖酐形式可减少泄漏和区室化、 BAPTA 偶联物用于检测高幅钙瞬变。更多信息请参阅 Molecular Probes™ 手册可见光激发的 Ca2+ 荧光指示剂—第 19.3 节

对于 UV 激发的 Ca2+ 指示剂、基于蛋白的 Ca2+ 指示剂、Ca2+ 指示剂的偶联物以及其他金属离子(即 Mg2+、Zn2+)的荧光指示剂,请查看 Molecular Probes™ 手册中的 Ca2+、Mg2+、Zn2+ 以及其他金属离子指示剂—第 19 章

仅供科研使用。不适用于动物或人类的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
数量5 mg
运输条件室温
适用于(应用)细胞活力与增殖
适用于(设备)荧光显微镜
产品线Calcium Green
产品类型钙指示剂
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

引用和文献 (106)

引用和文献
Abstract
Vesicle-associated proteins and calcium in nerve terminals of chick ciliary ganglia during development of facilitation.
Authors:Lin YQ, Brain KL, Nichol KA, Morgan JJ, Bennett MR
Journal:J Physiol
PubMed ID:9003550
'1. The developmental appearance of synaptic vesicle-associated proteins and nerve terminal calcium ([Ca2+]i) sequestering processes were determined for the chick ciliary ganglia in relation to the maturation of the different phase of increased efficacy of transmitter release following nerve impulses. The maturation phases studied were from stages 34-35, at the ... More
Ca2+ influx does more than provide releasable Ca2+ to maintain repetitive spiking in human umbilical vein endothelial cells.
Authors:Morgan AJ, Jacob R
Journal:Biochem J
PubMed ID:8973560
'We investigated why oscillations of intracellular Ca2+ concentrations ([Ca2+]i) in endothelial cells challenged by sub-maximal histamine run down in Ca(2+)-free medium despite stores retaining most of their Ca2+. One explantation is that only a small subpopulation of the Ca2+ stores oscillate and are completely emptied of Ca2+. To investigate if ... More
Maturation promoting factor in ascidian oocytes is regulated by different intracellular signals at meiosis I and II.
Authors:Russo GL, Kyozuka K, Antonazzo L, Tosti E, Dale B
Journal:Development
PubMed ID:8681780
'Using the fluorescent dye Calcium Green-dextran, we measured intracellular Ca2+ in oocytes of the ascidian Ciona intestinalis at fertilization and during progression through meiosis. The relative fluorescence intensity increased shortly after insemination in a single transient, the activation peak, and this was followed by several smaller oscillations that lasted for ... More
Calcium permeability of the neuronal nuclear envelope: evaluation using confocal volumes and intracellular perfusion.
Authors:O'Malley DM
Journal:J Neurosci
PubMed ID:7931542
'In many calcium-imaging studies, the nuclear envelope appears to maintain a gradient of free calcium between the nucleus and cytosol. This issue was examined by loading amphibian sympathetic neurons with the calcium indicator fluo 3 via whole-cell patch clamping. Confocal optical sectioning allowed acquisition of independent calibration curves for the ... More
Calcium signalling during chemotaxis.
Authors:Fay FS, Gilbert SH, Brundage RA
Journal:Ciba Found Symp
PubMed ID:7587614
'The role of Ca2+ in chemotaxis of eosinophils from the newt Taricha granulosa was investigated using fluorescent indicators and digital imaging microscopy. In response to serum chemoattractant, cytoplasmic Ca2+ concentration ([Ca2+]i) rises prior to polarization. In polarized locomoting cells [Ca2+]i gradients (tail-high-front-low) are always seen, and when cells turn [Ca2+]i ... More