Vesicle-associated proteins and calcium in nerve terminals of chick ciliary ganglia during development of facilitation.
Authors:Lin YQ, Brain KL, Nichol KA, Morgan JJ, Bennett MR
Journal:J Physiol
PubMed ID:9003550
'1. The developmental appearance of synaptic vesicle-associated proteins and nerve terminal calcium ([Ca2+]i) sequestering processes were determined for the chick ciliary ganglia in relation to the maturation of the different phase of increased efficacy of transmitter release following nerve impulses. The maturation phases studied were from stages 34-35, at the ... More
Ca2+ influx does more than provide releasable Ca2+ to maintain repetitive spiking in human umbilical vein endothelial cells.
Authors:Morgan AJ, Jacob R
Journal:Biochem J
PubMed ID:8973560
'We investigated why oscillations of intracellular Ca2+ concentrations ([Ca2+]i) in endothelial cells challenged by sub-maximal histamine run down in Ca(2+)-free medium despite stores retaining most of their Ca2+. One explantation is that only a small subpopulation of the Ca2+ stores oscillate and are completely emptied of Ca2+. To investigate if ... More
Maturation promoting factor in ascidian oocytes is regulated by different intracellular signals at meiosis I and II.
Authors:Russo GL, Kyozuka K, Antonazzo L, Tosti E, Dale B
Journal:Development
PubMed ID:8681780
'Using the fluorescent dye Calcium Green-dextran, we measured intracellular Ca2+ in oocytes of the ascidian Ciona intestinalis at fertilization and during progression through meiosis. The relative fluorescence intensity increased shortly after insemination in a single transient, the activation peak, and this was followed by several smaller oscillations that lasted for ... More
Calcium permeability of the neuronal nuclear envelope: evaluation using confocal volumes and intracellular perfusion.
Authors:O'Malley DM
Journal:J Neurosci
PubMed ID:7931542
'In many calcium-imaging studies, the nuclear envelope appears to maintain a gradient of free calcium between the nucleus and cytosol. This issue was examined by loading amphibian sympathetic neurons with the calcium indicator fluo 3 via whole-cell patch clamping. Confocal optical sectioning allowed acquisition of independent calibration curves for the ... More
Calcium signalling during chemotaxis.
Authors:Fay FS, Gilbert SH, Brundage RA
Journal:Ciba Found Symp
PubMed ID:7587614
'The role of Ca2+ in chemotaxis of eosinophils from the newt Taricha granulosa was investigated using fluorescent indicators and digital imaging microscopy. In response to serum chemoattractant, cytoplasmic Ca2+ concentration ([Ca2+]i) rises prior to polarization. In polarized locomoting cells [Ca2+]i gradients (tail-high-front-low) are always seen, and when cells turn [Ca2+]i ... More