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引用和文献 (26)
Invitrogen™
腔肠素 h
腔肠素 h 是几种合成腔肠素类似物之一，它们赋予水母发光蛋白复合物不同的 Ca2+ 亲和力和光谱特性。像天然腔肠素一样，这五种新型衍生物可用于体内和体外实验时重组水母发光蛋白复合物。了解更多有关离子指示剂（包括钙、钾、pH了解更多信息
| 货号 | 数量 |
|---|---|
| C6780 又称 C-6780 | 250 μg |
货号 C6780
又称 C-6780
价格（CNY)
4,858.00
Each
数量:
250 μg
价格（CNY)
4,858.00
Each
腔肠素 h 是几种合成腔肠素类似物之一，它们赋予水母发光蛋白复合物不同的 Ca2+ 亲和力和光谱特性。像天然腔肠素一样，这五种新型衍生物可用于体内和体外实验时重组水母发光蛋白复合物。
了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›
了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›
仅供科研使用。不可用于诊断程序。
规格
检测方法生物发光
染料类型基于蛋白质
数量250 μg
运输条件室温
适用于（设备）化学发光酶标仪（微孔板）, 化学发光酶标仪（试管型）
产品类型腔肠素 h
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。
引用和文献 (26)
引用和文献
Abstract
Intracellular Ca2+ signals in Dictyostelium chemotaxis are mediated exclusively by Ca2+ influx.
Journal:J Cell Sci
PubMed ID:9427292
'We measured folate- and cAMP-induced changes in cytoplasmic free calcium concentration ([Ca2+]i) using recombinant aequorin reconstituted in living Dictyostelium cells with coelenterazine-h. The resulting semi-synthetic protein displayed increased sensitivity to Ca2+ allowing accurate measurement of chemoattractant-induced transients at low resting levels. Both folate- and cAMP-induced Ca2+ responses were developmentally regulated,
Quantitative assessment of beta 1- and beta 2-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer.
Journal:J Biol Chem
PubMed ID:12244098
'Quantitative bioluminescence resonance energy transfer (BRET) analysis was applied to the study of beta(1)- and beta(2)-adrenergic receptor homo- and heterodimerization. To assess the relative affinity between each of the protomers, BRET saturation experiments were carried out in HEK-293T cells. beta(1)- and beta(2)-adrenergic receptors were found to have similar propensity to
The use of Renilla luciferase, Oplophorus luciferase, and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate.
Journal:Biochem Biophys Res Commun
PubMed ID:9144537
'To investigate the use of various luciferases as reporter protein, the substrate specificity of recombinant Renilla luciferase, Oplophorus luciferase and recombinant apoaequorin was examined using 23 kinds of coelenterazine analogues as the substrate. The intensity of luminescence was generally highest with Oplophorus luciferase and lowest with apoaequorin, but varied widely
Characterization of coelenterazine analogs for measurements of Renilla luciferase activity in live cells and living animals.
Journal:Mol Imaging
PubMed ID:15142411
'In vivo imaging of bioluminescent reporters relies on expression of light-emitting enzymes, luciferases, and delivery of chemical substrates to expressing cells. Coelenterazine (CLZN) is the substrate for a group of bioluminescent enzymes obtained from marine organisms. At present, there are more than 10 commercially available CLZN analogs. To determine which
Imaging [Ca2+]i with aequorin using a photon imaging detector.
Journal:Methods Cell Biol
PubMed ID:8201982