Novel use of the fluorescent dye 5-(and-6)-chloromethyl SNARF-1 acetate for the measurement of intracellular glutathione in leukemic cells and primary lymphocytes.
AuthorsHamilton D, Loignon M, Alaoui-Jamali MA, Batist G,
JournalCytometry A
PubMed ID17623874
'Glutathione (GSH) plays an important role in protecting cells against injury, particularly during oxidative stress. Alterations in GSH metabolism are becoming the focus of attention in many diseases such as cancer, neurodegeneration, and AIDS. As such, a rapid assessment of GSH levels in a clinical setting is of increasing importance. ... More
Cell-to-cell spread and massive vacuole formation after Cryptococcus neoformans infection of murine macrophages.
AuthorsAlvarez M, Casadevall A,
JournalBMC Immunol
PubMed ID17705844
'BACKGROUND: The interaction between macrophages and Cryptococcus neoformans (Cn) is critical for containing dissemination of this pathogenic yeast. However, Cn can either lyse macrophages or escape from within them through a process known as phagosomal extrusion. Both events result in live extracellular yeasts capable of reproducing and disseminating in the ... More
Fluorescent indicators for intracellular pH.
AuthorsHan J, Burgess K,
JournalChem Rev
PubMed ID19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More
A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
AuthorsCarman CV, Springer TA
JournalJ Cell Biol
PubMed ID15504916
The basic route and mechanisms for leukocyte migration across the endothelium remain poorly defined. We provide definitive evidence for transcellular (i.e., through individual endothelial cells) diapedesis in vitro and demonstrate that virtually all, both para- and transcellular, diapedesis occurs in the context of a novel "cuplike" transmigratory structure. This endothelial ... More
Enhanced cellular immunity in macaques following a novel peptide immunotherapy.
AuthorsChea S, Dale CJ, De Rose R, Ramshaw IA, Kent SJ
JournalJ Virol
PubMed ID15731268
Advances in treating and preventing AIDS depend on understanding how human immunodeficiency virus (HIV) is eliminated in vivo and on the manipulation of effective immune responses to HIV. During the development of assays quantifying the elimination of fluorescent autologous cells coated with overlapping 15-mer simian immunodeficiency virus (SIV) or HIV-1 ... More
Acellular hemoglobin solution enters compressed lung capillaries more readily than red blood cells.
AuthorsConhaim RL, Rodenkirch LA, Watson KE, Harms BA
JournalJ Appl Physiol
PubMed ID10956369
High lung inflation pressures compress alveolar septal capillaries, impede red cell transit, and interfere with oxygenation. However, recently introduced acellular hemoglobin solutions may enter compressed lung capillaries more easily than red blood cells. To test this hypothesis, we perfused isolated rat lungs with fluorescently labeled diaspirin cross-linked hemoglobin (DCLHb; 10%) ... More
Bcl-2 inhibitors sensitize tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by uncoupling of mitochondrial respiration in human leukemic CEM cells.
AuthorsHao JH, Yu M, Liu FT, Newland AC, Jia L
JournalCancer Res
PubMed ID15150119
Previous studies have shown that the lymphoblastic leukemia CEM cell line is resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis because of a low expression of caspase-8. Bcl-2 inhibitors, BH3I-2' and HA14-1, are small cell-permeable nonpeptide compounds, are able to induce apoptosis by mediating cytochrome c release, and also ... More