Can CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026) be used on organoids in Matrigel medium?
CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) has been validated for use with the AlgiMatrix 3D Culture System, but we cannot guarantee that it will work with other 3D culture systems.
What is the composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026)?
The composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture is proprietary.
Which cell proliferation assays can I use with 3D culture systems?
alamarBlue Cell Viability Reagent, PrestoBlue Cell Viability Reagent, and CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) have been validated for use with the AlgiMatrix 3D Culture System and should also work with other 3D culture systems. For further details, please refer to this poster (https://aacrjournals.org/cancerres/article/70/8_Supplement/3234/563757/Abstract-3234-AlgiMatrixT-3-D-cell-culture-system) and protocol (http://www.thermofisher.com/us/en/home/references/protocols/cell-culture/3-d-cell-culture-protocol/algimatrix-viability-using-cyquant.html).