CyQUANT™ Cell Lysis Buffer (20X), 50 mL - FAQs

Is the ATP Determination Kit suitable for frozen heart tissue, tissue homogenate, or serum or plasma (Cat. No. A22066)?

Yes, the ATP Determination Kit (Cat. No. A22066) can be used for tissue samples like frozen heart tissue, as well as blood serum and plasma samples.

Please note, you must physically homogenize the tissue and then lyse the cells using a low-detergent lysis buffer.

We offer the following low-detergent lysis buffer:

Pierce Luciferase Cell Lysis Buffer (2X) (Cat. No. 16189)

As an alternative, you can make your own 20X buffer using this recipe:

20X Cell lysis buffer:
200 mM Tris, pH 7.5
2 M NaCl
20 mM EDTA
0.2 % Triton X-100

Reference:

Ahn BH, Kim HS, Song S, Lee IH, Liu J, Vassilopoulos A, Deng CX, Finkel T. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14447-52. doi: 10.1073/pnas.0803790105. Epub 2008 Sep 15. PMID: 18794531; PMCID: PMC2567183.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.