Caged nicotinic acid adenine dinucleotide phosphate. Synthesis and use.
AuthorsLee HC, Aarhus R, Gee KR, Kestner T
JournalJ Biol Chem
PubMed ID9020130
'Nicotinic acid adenine dinucleotide phosphate (NAADP) is a metabolite of NADP with Ca2+ mobilizing activity. The Ca2+ release mechanism activated by NAADP as well as the Ca2+ stores that it acts on are different from those activated by either cyclic ADP-ribose or inositol 1,4,5-trisphosphate (IP3) (Lee, H. C., and Aarhus, ... More
Cyclic ADP-ribose does not regulate sarcoplasmic reticulum Ca2+ release in intact cardiac myocytes.
AuthorsGuo X, Laflamme MA, Becker PL
JournalCirc Res
PubMed ID8925563
'Cyclic ADP-ribose (cADPR), an intracellular second messenger known to mobilize Ca2+ in sea urchin eggs, has been implicated in modulating Ca2+ release in a variety of mammalian tissues. On the basis of studies of isolated cardiac sarcoplasmic reticulum (SR) vesicles and single SR Ca2+ release channels, cADPR has also been ... More
Cyclic ADP-ribose increases Ca2+ removal in smooth muscle.
'Ca2+ release via ryanodine receptors (RyRs) is vital in cell signalling and regulates diverse activities such as gene expression and excitation-contraction coupling. Cyclic ADP ribose (cADPR), a proposed modulator of RyR activity, releases Ca2+ from the intracellular store in sea urchin eggs but its mechanism of action in other cell ... More
Cyclic ADP-ribose-gated Ca2+ release in sea urchin eggs requires an elevated.
AuthorsGuo X, Becker PL
JournalJ Biol Chem
PubMed ID9202011
'Cyclic ADP-ribose (cADPr) has been shown to release intracellular Ca2+ from sea urchin eggs and a variety of vertebrate cell types, although its mechanism of action remains elusive. We employed the caged version of cADPr to study the [Ca2+] transient kinetics in intact sea urchin eggs for insights into how ... More
Prolonged inactivation of nicotinic acid adenine dinucleotide phosphate-induced Ca2+ release mediates a spatiotemporal Ca2+ memory.
AuthorsChurchill GC, Galione A
JournalJ Biol Chem
PubMed ID11139579
'Although numerous extracellular stimuli are coupled to increases in intracellular Ca(2+), different stimuli are thought to achieve specificity by eliciting different spatiotemporal Ca(2+) increases. We investigated the effect of nicotinic acid adenine dinucleotide phosphate (NAADP) inactivation on spatiotemporal Ca(2+) signals in intact sea urchin eggs. The photorelease of NAADP but ... More
Preparation of cyclic ADP-ribose antagonists and caged cyclic ADP-ribose.
AuthorsWalseth TF, Aarhus R, Gurnack ME, Wong L, Breitinger HG, Gee KR, Lee HC
JournalMethods Enzymol
PubMed ID9211325
Intercellular calcium signaling and flash photolysis of caged compounds. A sensitive method to evaluate gap junctional coupling.
AuthorsLeybaert L, Sanderson MJ
JournalMethods Mol Biol
PubMed ID11218662
Caged compounds: photorelease technology for control of cellular chemistry and physiology.
AuthorsEllis-Davies GC
JournalNat Methods
PubMed ID17664946
Caged compounds are light-sensitive probes that functionally encapsulate biomolecules in an inactive form. Irradiation liberates the trapped molecule, permitting targeted perturbation of a biological process. Uncaging technology and fluorescence microscopy are 'optically orthogonal': the former allows control, and the latter, observation of cellular function. Used in conjunction with other technologies ... More
NAADP mobilizes Ca(2+) from reserve granules, lysosome-related organelles, in sea urchin eggs.
AuthorsChurchill GC, Okada Y, Thomas JM, Genazzani AA, Patel S, Galione A
JournalCell
PubMed ID12464181
Nicotinic acid adenine dinucleotide phosphate (NAADP) mobilizes Ca(2+) in many cells and species. Unlike other Ca(2+)-mobilizing messengers, NAADP mobilizes Ca(2+) from an unknown store that is not the endoplasmic reticulum, the store traditionally associated with messenger-mediated Ca(2+) signaling. Here, we demonstrate the presence of a Ca(2+) store in sea urchin ... More
Bioorganic chemistry of cyclic ADP-ribose (cADPR).
AuthorsZhang FJ, Gu QM, Sih CJ
JournalBioorg Med Chem
PubMed ID10400320
The objective of this brief review is to present an overview of the bioorganic chemistry of cyclic-ADP-ribose (cADPR) with special emphasis on the methodology used for the synthesis of analogues of cADPR. New structural analogues of cADPR can be prepared using either the biomimetic method or ADP-ribosyl cyclase from Aplysia ... More
Effects of 1-methyladenine on nuclear Ca2+ transients and meiosis resumption in starfish oocytes are mimicked by the nuclear injection of inositol 1,4,5-trisphosphate and cADP-ribose.
AuthorsSantella L, Kyozuka K
JournalCell Calcium
PubMed ID9232348
The treatment of prophase-arrested starfish oocytes with the hormone 1-methyladenine (1-MA) induces the elevation of Ca2+ in both the cytoplasm and the germinal vesicle (nucleus), and is followed by the resumption of meiosis. The injection of the modulators of the intracellular Ca2+ channels inositol 1,4,5-trisphosphate (InsP3) or cyclic adenosine diphosphate ... More
Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells.
AuthorsYamasaki M, Masgrau R, Morgan AJ, Churchill GC, Patel S, Ashcroft SJ, Galione A
JournalJ Biol Chem
PubMed ID14660554
How different extracellular stimuli can evoke different spatiotemporal Ca2+ signals is uncertain. We have elucidated a novel paradigm whereby different agonists use different Ca2+-storing organelles ("organelle selection") to evoke unique responses. Some agonists select the endoplasmic reticulum (ER), and others select lysosome-related (acidic) organelles, evoking spatial Ca2+ responses that mirror ... More
Nicotinic acid-adenine dinucleotide phosphate mobilizes Ca2+ from a thapsigargin-insensitive pool.
AuthorsGenazzani AA, Galione A
JournalBiochem J
PubMed ID8645149
Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a novel intracellular Ca2+ releasing agent recently described in sea-urchin eggs and egg homogenates. Ca2+ release by NAADP is independent of that induced by either inositol trisphosphate (InsP3) or cyclic adenosine dinucleotide phosphate (cADPR). We now report that in sea urchin egg homogenates, NAADP ... More
Caged cyclic ADP-ribose. Synthesis and use.
AuthorsAarhus R, Gee K, Lee HC
JournalJ Biol Chem
PubMed ID7706323
Cyclic ADP-ribose (cADPR) is a recently discovered cyclic nucleotide with Ca2+ mobilizing activity. Caged cADPR was synthesized by reacting cADPR with 2-nitrophenethyldiazoethane. Elemental analyses, 1H NMR, and extinction coefficient measurements indicate that the product contains only one caging group. Anion exchange high pressure liquid chromatography separated caged cADPR into two ... More
Dual effects of cyclic ADP-ribose on sarcoplasmic reticulum Ca2+ release and storage in cardiac myocytes isolated from guinea-pig and rat ventricle.
AuthorsMacgregor AT, Rakovic S, Galione A, Terrar DA
JournalCell Calcium
PubMed ID17156839
The actions of cyclic ADP-ribose (cADPR), a regulator of Ca2+-induced Ca2+ release (CICR), were investigated on Ca2+ release and sarcoplasmic reticulum (SR) Ca2+ loading in cardiac myocytes at physiological temperature. In guinea-pig ventricular cells, cADPR, applied via patch pipette or from photorelease of its caged derivative, increased contraction amplitude and ... More
Ca2+ signalling and membrane current activated by cADPr in starfish oocytes.
AuthorsMoccia F, Nusco GA, Lim D, Ercolano E, Gragnaniello G, Brown ER, Santella L
JournalPflugers Arch
PubMed ID12756567
Cyclic ADP-ribose (cADPr) is a second messenger that regulates intracellular free [Ca2+] ([Ca2+](i)) in a variety of cell types, including immature oocytes from the starfish Astropecten auranciacus. In this study, we employed confocal laser scanning microscopy and voltage clamp techniques to investigate the source of the cADPr-elicited Ca2+ wave originating ... More
Association of calmodulin with nuclear structures in starfish oocytes and its role in the resumption of meiosis.
AuthorsSantella L, Kyozuka K
JournalEur J Biochem
PubMed ID9219515
The resumption of meiosis in prophase-arrested starfish oocytes is induced by the hormone 1-methyladenine, which has been shown previously to induce a calcium transient in the nucleus which at this stage is called the germinal vesicle. This transient precedes the breakdown of the germinal vesicle (GVBD). Experiments were performed to ... More
Effects of photoreleased cADP-ribose on calcium transients and calcium sparks in myocytes isolated from guinea-pig and rat ventricle.
AuthorsCui Y, Galione A, Terrar DA
JournalBiochem J
PubMed ID10455010
Actions of photoreleased cADP-ribose (cADPR), a novel regulator of calcium-induced calcium release (CICR) from ryanodine-sensitive stores, were investigated in cardiac myocytes. Photoreleased cADPR caused an increase in the magnitude of whole-cell calcium transients studied in mammalian cardiac ventricular myocytes (both guinea-pig and rat) using confocal microscopy). Approx. 15 s was ... More
Mechanisms of calcium signaling by cyclic ADP-ribose and NAADP.
AuthorsLee HC
JournalPhysiol Rev
PubMed ID9354813
Cells possess various mechanisms for transducing external signals to intracellular responses. The discovery of inositol 1,4,5-trisphosphate (IP3) as a messenger for mobilizing internal Ca2+ stores has centralized Ca2+ mobilization among signaling mechanisms. Results reviewed in this article establish that, in addition to IP3, the internal Ca2+ stores can be mobilized ... More
Detection and functional characterization of ryanodine receptors from sea urchin eggs.
AuthorsLokuta AJ, Darszon A, Beltrán C, Valdivia HH
JournalJ Physiol
PubMed ID9625874
1. Immunoblot analysis, [3H]ryanodine binding, and planar lipid bilayer techniques were used to identify and characterize the functional properties of ryanodine receptors (RyRs) from Lytechinus pictus and Strongylocentrotus purpuratus sea urchin eggs. 2. An antibody against mammalian skeletal RyRs identified an approximately 400 kDa band in the cortical microsomes of ... More