Novel flow cytometry compensation standards: internally stained fluorescent microspheres with matched emission spectra and long-term stability.
AuthorsZhang YZ, Kemper C, Bakke A, Haugland RP
JournalCytometry
PubMed ID9773886
'In flow cytometry, the emission spectral overlap of fluorescein and R-phycoerythrin is usually corrected by electronic color compensation using microspheres surface labeled with the same fluorochromes. However, the inherent chemical instability of these fluorochromes may cause inaccurate compensation. To overcome these problems, Compen-Flow beads, a new type of compensation standards ... More