Fluorescently labeled model DNA sequences for exonucleolytic sequencing.
AuthorsFöldes-Papp Z, Angerer B, Thyberg P, Hinz M, Wennmalm S, Ankenbauer W, Seliger H, Holmgren A, Rigler R
JournalJ Biotechnol
PubMed ID11257532
'We describe here the enzyme-catalyzed, low-density labeling of DNAs with fluorescent dyes. Firstly, for "natural" template DNAs, dNTPs were partially substituted in the labeling reactions by the respective fluorophore-bearing analogs. The DNAs were labeled by PCR using Taq DNA polymerase. The covalent incorporation of dye-dNTPs decreased in the following order: ... More
RNA structure analysis assisted by capillary electrophoresis.
AuthorsSobczak K, Krzyzosiak WJ
JournalNucleic Acids Res
PubMed ID12434006
'Although most capillary electrophoresis (CE) applications in the nucleic acid field performed so far address DNA analysis, various RNA assays by CE have also been done. Both natural and synthetic RNAs have been examined to evaluate their quantities, sizes and interactions. In this report, we show a novel application of ... More
Comparative genomic hybridization with lissamine- and fluorescein-labeled nucleotides.
AuthorsRosenberg C, van Gijlswijk RP, Vos CB, Wiegant J, Cornelisse CJ, Tanke HJ, Raap AK
JournalCytometry
PubMed ID9701403
'Biotin deoxyuridine 5''-triphosphate (dUTP) and digoxigenin dUTP are the labels most commonly used in comparative genomic hybridization (CGH). The relative infrequent use of direct fluorochrome-labeled nucleotides in CGH is related to the lower sensitivity they provide. Here we report the evaluation of two fluorochrome-conjugated nucleotides that have not been previously ... More
Precursor RNAs harboring nonsense codons accumulate near the site of transcription.
AuthorsMühlemann O, Mock-Casagrande CS, Wang J, Li S, Custódio N, Carmo-Fonseca M, Wilkinson MF, Moore MJ
JournalMol Cell
PubMed ID11511358
Messenger RNAs containing premature termination codons (PTCs) are selectively eliminated by nonsense-mediated mRNA decay (NMD). Paradoxically, although cytoplasmic ribosomes are the only known species capable of PTC recognition, in mammals many PTC-containing mRNAs are apparently eliminated prior to release from the nucleus. To determine whether PTCs can influence events within ... More
An evaluation of a new series of fluorescent dUTPs for fluorescence in situ hybridization.
AuthorsWiegant J, Verwoerd N, Mascheretti S, Bolk M, Tanke HJ, Raap AK
JournalJ Histochem Cytochem
PubMed ID8627009
Synthesis of fluorochrome-modified deoxyribonucleotides has been carried out mostly by linking the fluorochrome molecule to the C-5 position of dUTP via an allylamine spacer, similar to the modification of allylamine-dUTP with the haptens biotin and digoxigenin. Recently, a new series of fluorescent nucleotides has been prepared by using an alkynyl ... More