Multicolor spectral karyotyping of human chromosomes.
AuthorsSchröck E, du Manoir S, Veldman T, Schoell B, Wienberg J, Ferguson-Smith MA, Ning Y, Ledbetter DH, Bar-Am I, Soenksen D, Garini Y, Ried T
JournalScience
PubMed ID8662537
'The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally ... More
An evaluation of a new series of fluorescent dUTPs for fluorescence in situ hybridization.
AuthorsWiegant J, Verwoerd N, Mascheretti S, Bolk M, Tanke HJ, Raap AK
JournalJ Histochem Cytochem
PubMed ID8627009
Synthesis of fluorochrome-modified deoxyribonucleotides has been carried out mostly by linking the fluorochrome molecule to the C-5 position of dUTP via an allylamine spacer, similar to the modification of allylamine-dUTP with the haptens biotin and digoxigenin. Recently, a new series of fluorescent nucleotides has been prepared by using an alkynyl ... More
Hidden chromosome abnormalities in haematological malignancies detected by multicolour spectral karyotyping.
AuthorsVeldman T, Vignon C, Schröck E, Rowley JD, Ried T
JournalNat Genet
PubMed ID9090389
Cytogenetic analysis provides critical information of diagnostic and prognostic importance for haematological malignancies. In fact, the identification of recurring chromosomal breakpoints in leukaemias and lymphomas has expedited the cloning of genes whose translocation-induced deregulation causes malignant transformation. The pillar of karyotype analysis rests on chromosome banding techniques that have the ... More