Developmental shift in the relative percentages of lung fibroblast subsets: role of apoptosis postseptation.
AuthorsAwonusonu F,Srinivasan S,Strange J,Al-Jumaily W,Bruce MC
JournalThe American journal of physiology
PubMed ID10516228
Authors
Journal
PubMed ID9922213
Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color.
AuthorsLi X, Melamed MR, Darzynkiewicz Z
JournalExp Cell Res
PubMed ID8549670
'Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated nucleotides has been recently proposed as a means of analyzing DNA replication and detecting proliferating cells. The presence of numerous in situ DNA strand breaks is also an inherent feature of apoptotic cells. The aim of ... More
Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation.
AuthorsLi X, Darzynkiewicz Z
JournalCell Prolif
PubMed ID8555370
'In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing DNA replication. Detection of DNA strand breaks, thus, enables one ... More
Homologous recombinational repair factors are recruited and loaded onto the viral DNA genome in Epstein-Barr virus replication compartments.
AuthorsKudoh A, Iwahori S, Sato Y, Nakayama S, Isomura H, Murata T, Tsurumi T,
JournalJ Virol
PubMed ID19386720
Homologous recombination is an important biological process that facilitates genome rearrangement and repair of DNA double-strand breaks (DSBs). The induction of Epstein-Barr virus (EBV) lytic replication induces ataxia telangiectasia-mutated (ATM)-dependent DNA damage checkpoint signaling, leading to the clustering of phosphorylated ATM and Mre11/Rad50/Nbs1 (MRN) complexes to sites of viral genome ... More
Crucial mitochondrial impairment upon CDC48 mutation in apoptotic yeast.
Mutation in CDC48 (cdc48(S565G)), a gene essential in the endo-plasmic reticulum (ER)-associated protein degradation (ERAD) pathway, led to the discovery of apoptosis as a mechanism of cell death in the unicellular organism Saccharomyces cerevisiae. Elucidating Cdc48p-mediated apoptosis in yeast is of particular interest, because Cdc48p is the highly conserved yeast ... More
Advances in cytochemical methods for detection of apoptosis.
AuthorsBarrett KL, Willingham JM, Garvin AJ, Willingham MC
JournalJ Histochem Cytochem
PubMed ID11410607
In an earlier article from this laboratory, the current methods developed to detect apoptosis in cells and tissues were highlighted, along with the challenges in their interpretation. Recent discoveries concerning the underlying biochemical mechanisms of apoptotic effector pathways have made possible further assays that allow a more direct measure of ... More
Single-step procedure for labeling DNA strand breaks with fluorescein- or BODIPY-conjugated deoxynucleotides: detection of apoptosis and bromodeoxyuridine incorporation.
AuthorsLi X, Traganos F, Melamed MR, Darzynkiewicz Z
JournalCytometry
PubMed ID7664627
The methods of in situ labeling of DNA strand breaks have been used to identify apoptotic cells and/or DNA replicating cells. While discrimination of apoptotic cells is based on the inherent presence of numerous DNA strand breaks in their chromatin, DNA proliferating cells can be discriminated by the selective DNA ... More
Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis).
AuthorsDarzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F
JournalCytometry
PubMed ID9000580
The term cell necrobiology is introduced to comprise the life processes associated with morphological, biochemical, and molecular changes which predispose, precede, and accompany cell death, as well as the consequences and tissue response to cell death. Two alternative modes of cell death can be distinguished, apoptosis and accidental cell death, ... More
Mitochondrial membrane potential and nuclear changes in apoptosis caused by serum and nerve growth factor withdrawal: time course and modification by (-)-deprenyl.
AuthorsWadia JS, Chalmers-Redman RM, Ju WJ, Carlile GW, Phillips JL, Fraser AD, Tatton WG
JournalJ Neurosci
PubMed ID9437015
Studies in non-neural cells have suggested that a fall in mitochondrial membrane potential (DeltaPsiM) is one of the earliest events in apoptosis. It is not known whether neural apoptosis caused by nerve growth factor (NGF) and serum withdrawal involves a decrease in DeltaPsiM. We used epifluorescence and laser confocal microscopy ... More