tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells.
AuthorsMhlanga MM, Vargas DY, Fung CW, Kramer FR, Tyagi S
JournalNucleic Acids Res
PubMed ID15809226
'When oligonucleotide probes are microinjected into cells to image the distribution of RNAs, they are rapidly sequestered into the nucleus. As a result, it is difficult to detect mRNAs in the cytoplasm of living cells. We were able to overcome this process by attaching tRNA transcripts to the probes. We ... More
Ribonuclease H renaturation gel assay using a fluorescent-labeled substrate.
AuthorsHan LY, Ma WP, Crouch RJ
JournalBiotechniques
PubMed ID9383560
Ribonucleases H (RNases H) are enzymes that specifically degrade the RNA of RNA-DNA hybrids. These enzymes are involved in DNA replication, reverse transcription (RT) and antisense oligodeoxyribonucleotide-mediated arrest of translation. One of the most valuable tools for assaying RNase H activity is the renaturation gel assay with which such activities ... More