A Xenopus protein related to hnRNP I has a role in cytoplasmic RNA localization.
AuthorsCote CA, Gautreau D, Denegre JM, Kress TL, Terry NA, Mowry KL
JournalMol Cell
PubMed ID10518224
Cytoplasmic localization of mRNA molecules is a powerful mechanism for generating cell polarity. In vertebrates, one paradigm is localization of Vg1 RNA within the Xenopus oocyte, a process directed by recognition of a localization element within the Vg1 3' UTR. We show that specific base changes within the localization element ... More
Direct imaging of DNA in living cells reveals the dynamics of chromosome formation.
AuthorsManders EM, Kimura H, Cook PR
JournalJ Cell Biol
PubMed ID10085283
Individual chromosomes are not directly visible within the interphase nuclei of most somatic cells; they can only be seen during mitosis. We have developed a method that allows DNA strands to be observed directly in living cells, and we use it to analyze how mitotic chromosomes form. A fluorescent analogue ... More
Ribonuclease H renaturation gel assay using a fluorescent-labeled substrate.
AuthorsHan LY, Ma WP, Crouch RJ
JournalBiotechniques
PubMed ID9383560
Ribonucleases H (RNases H) are enzymes that specifically degrade the RNA of RNA-DNA hybrids. These enzymes are involved in DNA replication, reverse transcription (RT) and antisense oligodeoxyribonucleotide-mediated arrest of translation. One of the most valuable tools for assaying RNase H activity is the renaturation gel assay with which such activities ... More