A self-restricted CD38-connexin 43 cross-talk affects NAD+ and cyclic ADP-ribose metabolism and regulates intracellular calcium in 3T3 fibroblasts.
AuthorsBruzzone S, Franco L, Guida L, Zocchi E, Contini P, Bisso A, Usai C, De Flora A
JournalJ Biol Chem
PubMed ID11602597
'Connexin 43 (Cx43) hexameric hemichannels, recently demonstrated to mediate NAD(+) transport, functionally interact in the plasma membrane of several cells with the ectoenzyme CD38 that converts NAD(+) to the universal calcium mobilizer cyclic ADP-ribose (cADPR). Here we demonstrate that functional uncoupling between CD38 and Cx43 in CD38-transfected 3T3 murine fibroblasts ... More
Synergistic calcium release in the sea urchin egg by ryanodine and cyclic ADP ribose.
AuthorsBuck WR, Hoffmann EE, Rakow TL, Shen SS
JournalDev Biol
PubMed ID8174765
'A transient rise in cytoplasmic Ca2+ activity in the sea urchin egg occurs during fertilization due to release from an intracellular store. Two intracellular receptor Ca2+ channels for inositol 1,4,5-trisphosphate (IP3) and ryanodine have been identified by physiological and immunological techniques. While IP3 is the endogenous messenger for the IP3 ... More
cADP-ribose formation by blood platelets is not responsible for intracellular calcium mobilization.
AuthorsOhlmann P, Leray C, Ravanat C, Hallia A, Cassel D, Cazenave JP, Gachet C
JournalBiochem J
PubMed ID9531481
'Human platelet CD38 is a multifunctional ectoenzyme catalysing the synthesis and hydrolysis of cADP-ribose (cADPR), a recently identified calcium-mobilizing agent that acts independently of D-myo-inositol 1,4,5-trisphosphate and is known to be expressed by human platelets. The present work shows that ADP-ribosyl cyclase activity is exclusively a membrane activity, of which ... More
Cyclic ADP-ribose and calcium-induced calcium release regulate neurotransmitter release at a cholinergic synapse of Aplysia.
'1. Presynaptic injection of cyclic ADP-ribose (cADPR), a modulator of the ryanodine receptor, increased the postsynaptic response evoked by a presynaptic spike at an identified cholinergic synapse in the buccal ganglion of Aplysia californica. 2. The statistical analysis of long duration postsynaptic responses evoked by square depolarizations of the voltage-clamped ... More
The two intracellular Ca2+ release channels, ryanodine receptor and inositol 1,4,5-trisphosphate receptor, play different roles during fertilization in ascidians.
AuthorsAlbrieux M, Sardet C, Villaz M
JournalDev Biol
PubMed ID9299112
'Fertilization in the ascidians triggers an activation wave of calcium release followed by intracellular calcium oscillations synchronous with periodic membrane potential excursions during the completion of the meiotic cell cycle. Fertilization also causes a fast decrease in the egg plasma membrane depolarization-activated calcium current and a large increase in capacitance ... More
Enzymatic synthesis and characterizations of cyclic GDP-ribose. A procedure for distinguishing enzymes with ADP-ribosyl cyclase activity.
AuthorsGraeff RM, Walseth TF, Fryxell K, Branton WD, Lee HC
JournalJ Biol Chem
PubMed ID7982936
'Cyclic nucleotides such as cAMP and cGMP are second messengers subserving various signaling pathways. Cyclic ADP-ribose (cADPR), a recently discovered member of the family, is derived from NAD+ and is a mediator of Ca2+ mobilization in various cellular systems. The synthesis and degradation of cADPR are, respectively, catalyzed by ADP-ribosyl ... More
Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.
AuthorsNolkrantz K, Farre C, Hurtig KJ, Rylander P, Orwar O
JournalAnal Chem
PubMed ID12199607
'A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased ... More
Imaging of intracellular calcium stores in single permeabilized lens cells.
AuthorsChurchill GC, Louis CF
JournalAm J Physiol
PubMed ID9950770
'Intracellular Ca2+ stores in permeabilized sheep lens cells were imaged with mag-fura 2 to characterize their distribution and sensitivity to Ca2+-releasing agents. Inositol 1,4,5-trisphosphate (IP3) or cyclic ADP-ribose (cADPR) released Ca2+ from intracellular Ca2+ stores that were maintained by an ATP-dependent Ca2+ pump. The IP3 antagonist heparin inhibited IP3- but ... More
All-trans-retinoic acid stimulates synthesis of cyclic ADP-ribose in renal LLC-PK1 cells.
AuthorsBeers KW, Chini EN, Dousa TP
JournalJ Clin Invest
PubMed ID7537765
'Cyclic adenosine diphospho-ribose (cADPR) triggers Ca2+ release from intracellular stores and is therefore proposed to function as a second messenger in cellular signaling; however, an extracellular stimulus, i.e., first messenger (hormone or autacoid) that modulates cADPR metabolism has not been identified. We discovered that all-trans-retinoic acid (atRA) is a potent ... More
A cADP-ribose antagonist does not inhibit secretagogue-, caffeine- and nitric oxide-induced Ca2+ responses in rat pancreatic beta-cells.
AuthorsWillmott NJ, Galione A, Smith PA
JournalCell Calcium
PubMed ID8581969
'It is controversial whether the Ca2+ mobilizing agent, cADP-ribose (cADPR), is implicated in secretagogue-mediated intracellular Ca2+ responses of pancreatic beta-cells. In this study we utilised a potent antagonist of cADPR, 8-amino-cADPR, to determine whether cADPR is involved in glucose-, acetylcholine-, caffeine- and nitric oxide-induced intracellular Ca2+ responses of isolated rat ... More
In situ characterization of nonmitochondrial Ca2+ stores in individual pancreatic beta-cells.
AuthorsTengholm A, Hagman C, Gylfe E, Hellman B
JournalDiabetes
PubMed ID9703321
'Free Ca2+ was measured in intracellular stores of individual mouse pancreatic beta-cells using dual-wavelength microfluorometry and the low-affinity Ca2+ indicator furaptra. Controlled permeabilization of the plasma membrane with 4 micromol/l digitonin revealed that 22% of the furaptra was trapped in intracellular nonnuclear compartments. When 3 mmol/l ATP and 200 nmol/l ... More
Synthesis and characterization of antagonists of cyclic-ADP-ribose-induced Ca2+ release.
AuthorsWalseth TF, Lee HC
JournalBiochim Biophys Acta
PubMed ID8395888
'Cyclic ADP-ribose (cADPR) is a naturally-occurring metabolite of NAD+ that is as effective as inositol trisphosphate in mobilizing intracellular Ca2+. A series of analogs modified at the 8-position of the adenine group were synthesized for the investigation of the relationship between the structure of the metabolite and its Ca(2+)-mobilizing activity. ... More
Specific modulation of cyclic ADP-ribose-induced Ca2+ release by polyamines.
AuthorsChini EN, Beers KW, Chini CC, Dousa TP
JournalAm J Physiol
PubMed ID7485444
'Cyclic ADP-ribose (cADPR) is a potent mediator of Ca2+ mobilization from intracellular stores in sea urchin eggs. However, the regulation of the cADPR-induced Ca2+ release system is not yet fully elucidated. We now report that spermine and related polyamines, in physiological concentrations, were able to inhibit the Ca2+ release induced ... More
cADP-ribose releases Ca2+ from cardiac sarcoplasmic reticulum independently of ryanodine receptor.
AuthorsLahouratate P, Guibert J, Faivre JF
JournalAm J Physiol
PubMed ID9321792
'Cyclic ADP-ribose (cADPR), an endogenous metabolite of beta-NAD+, activates Ca2+ release from endoplasmic reticulum in sea urchin eggs via the ryanodine receptor (RyR) pathway. A similar role has been proposed in cardiac sarcoplasmic reticulum (SR), although this remains controversial. We therefore investigated the ability of cADPR to induce Ca2+ release ... More
'In this study, we have identified and partially characterized a mouse T-lymphoma ryanodine receptor on a unique type of internal vesicle which bands at the relatively light density of 1.07 g/ml. Analysis of the binding of [3H]ryanodine to these internal vesicles reveals the presence of a single, low affinity binding ... More
Cyclic ADP-ribose in insulin secretion from pancreatic beta cells.
AuthorsTakasawa S, Nata K, Yonekura H, Okamoto H
JournalScience
PubMed ID8420005
'Inositol 1,4,5-trisphosphate (IP3) is thought to be a second messenger for intracellular calcium mobilization. However, in a cell-free system of islet microsomes, cyclic adenosine diphosphate-ribose (cADP-ribose), a nicotinamide adenine dinucleotide (NAD+) metabolite, but not IP3, induced calcium release. In digitonin-permeabilized islets, cADP-ribose and calcium, but not IP3, induced insulin secretion. ... More
Nitric oxide-induced mobilization of intracellular calcium via the cyclic ADP-ribose signaling pathway.
AuthorsWillmott N, Sethi JK, Walseth TF, Lee HC, White AM, Galione A
JournalJ Biol Chem
PubMed ID8631983
'Cyclic adenosine diphosphate ribose (cADPR) is a potent endogenous calcium-mobilizing agent synthesized from beta-NAD+ by ADP-ribosyl cyclases in sea urchin eggs and in several mammalian cells (Galione, A., and White, A. (1994) Trends Cell Biol. 4, 431 436). Pharmacological studies suggest that cADPR is an endogenous modulator of Ca2+-induced Ca2+ ... More
Activation and inactivation of Ca2+ release by NAADP+.
AuthorsAarhus R, Dickey DM, Graeff RM, Gee KR, Walseth TF, Lee HC
JournalJ Biol Chem
PubMed ID8621471
'Nicotinic acid adenine dinucleotide phosphate (NAADP+) is a recently identified metabolite of NADP+ that is as potent as inositol trisphosphate (IP3) and cyclic ADP-ribose (cADPR) in mobilizing intracellular Ca2+ in sea urchin eggs and microsomes (Clapper, D. L., Walseth, T. F., Dargie, P. J., and Lee, H. C. (1987) J. ... More
Extracellular cyclic ADP-ribose increases intracellular free calcium concentration and stimulates proliferation of human hemopoietic progenitors.
AuthorsPodestà M, Zocchi E, Pitto A, Usai C, Franco L, Bruzzone S, Guida L, Bacigalupo A, Scadden DT, Walseth TF, De Flora A, Daga A
JournalFASEB J
PubMed ID10744625
'Cyclic ADP-ribose (cADPR) is a universal second messenger that regulates many calcium-related cellular events by releasing calcium from intracellular stores. Since these events include enhanced cell proliferation and since the bone marrow harbors both ectoenzymes that generate cADPR from NAD(+) (CD38 and BST-1), we investigated the effects of extracellular cADPR ... More
Polarized expression of Ca2+ pumps in pancreatic and salivary gland cells. Role in initiation and propagation of [Ca2+]i waves.
AuthorsLee MG, Xu X, Zeng W, Diaz J, Kuo TH, Wuytack F, Racymaekers L, Muallem S
JournalJ Biol Chem
PubMed ID9188473
'The present study was aimed at localization of plasma membrane (PMCA) and intracellular (SERCA) Ca2+ pumps and characterizing their role in initiation and propagation of Ca2+ waves. Specific and polarized expression of Ca2+ pumps was observed in all epithelial cells examined. Immunolocalization revealed expression of PMCA in both the basolateral ... More
Vasodilation by the calcium-mobilizing messenger cyclic ADP-ribose.
AuthorsBoittin FX, Dipp M, Kinnear NP, Galione A, Evans AM
JournalJ Biol Chem
PubMed ID12486132
'In artery smooth muscle, adenylyl cyclase-coupled receptors such as beta-adrenoceptors evoke Ca(2+) signals, which open Ca(2+)-activated potassium (BK(Ca)) channels in the plasma membrane. Thus, blood pressure may be lowered, in part, through vasodilation due to membrane hyperpolarization. The Ca(2+) signal is evoked via ryanodine receptors (RyRs) in sarcoplasmic reticulum proximal ... More
Calcium-induced calcium release in neurones.
AuthorsVerkhratsky A, Shmigol A
JournalCell Calcium
PubMed ID8653752
'Neurones express several subtypes of intracellular Ca2+ channels, which are regulated by cytoplasmic calcium concentration ([Ca2+]c) and provide the pathway for Ca(2+)-induced Ca2+ release (CICR) from endoplasmic reticulum Ca2+ stores. The initial studies of CICR which employed several pharmacological tools (and in particular caffeine and ryanodine) demonstrated that: (i) caffeine ... More
Inositol trisphosphate and cyclic ADP-ribose-mediated release of Ca2+ from single isolated pancreatic zymogen granules.
'In pancreatic acinar cells low (physiological) agonist concentrations evoke cytosolic Ca2+ spikes specifically in the apical secretory pole that contains a high density of secretory (zymogen) granules (ZGs). Inositol 1,4,5-trisphosphate (IP3) is believed to release Ca2+ from the endoplasmic reticulum, but we have now tested whether the Ca(2+)-releasing messengers IP3 ... More
Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38.
AuthorsHoward M, Grimaldi JC, Bazan JF, Lund FE, Santos-Argumedo L, Parkhouse RM, Walseth TF, Lee HC
JournalScience
PubMed ID8235624
'CD38 is a 42-kilodalton glycoprotein expressed extensively on B and T lymphocytes. CD38 exhibits a structural homology to Aplysia adenosine diphosphate (ADP)-ribosyl cyclase. This enzyme catalyzes the synthesis of cyclic ADP-ribose (cADPR), a metabolite of nicotinamide adenine dinucleotide (NAD+) with calcium-mobilizing activity. A complementary DNA encoding the extracellular domain of ... More
Cyclic ADP-ribose and inositol 1,4,5-triphosphate mobilizes Ca2+ from distinct intracellular pools in permeabilized lacrimal acinar cells.
AuthorsGromada J, Jørgensen TD, Dissing S
JournalFEBS Lett
PubMed ID7883052
'In permeabilized lacrimal acinar cells, cyclic ADP-ribose (cADP-ribose) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) release Ca2+ in a dose dependent manner from distinct thapsigargin-sensitive Ca2+ pools. Ryanodine specifically blocks the Ca2+ response to cADP-ribose, whereas heparin strongly reduces the response to Ins(1,4,5)P3 application. GTP causes a rapid Ca2+ release by a ryanodine- ... More
Cyclic ADP-ribose-gated Ca2+ release in sea urchin eggs requires an elevated.
AuthorsGuo X, Becker PL
JournalJ Biol Chem
PubMed ID9202011
'Cyclic ADP-ribose (cADPr) has been shown to release intracellular Ca2+ from sea urchin eggs and a variety of vertebrate cell types, although its mechanism of action remains elusive. We employed the caged version of cADPr to study the [Ca2+] transient kinetics in intact sea urchin eggs for insights into how ... More
Cyclic ADP-ribose binds to FK506-binding protein 12.6 to release Ca2+ from islet microsomes.
AuthorsNoguchi N, Takasawa S, Nata K, Tohgo A, Kato I, Ikehata F, Yonekura H, Okamoto H
JournalJ Biol Chem
PubMed ID9013543
'Cyclic ADP-ribose (cADPR) is a second messenger for Ca2+ mobilization via the ryanodine receptor (RyR) from islet microsomes for insulin secretion (Takasawa, S., Nata, K., Yonekura, H., and Okamoto, H. (1993) Science 259, 370-373). In the present study, FK506, an immunosuppressant that prolongs allograft survival, as well as cADPR were ... More
A Ca2+ release mechanism gated by the novel pyridine nucleotide, NAADP.
AuthorsGenazzani AA, Galione A
JournalTrends Pharmacol Sci
PubMed ID9149538
Cell surface ADP-ribosyltransferase regulates lymphocyte function-associated molecule-1 (LFA-1) function in T cells.
AuthorsNemoto E, Yu Y, Dennert G
JournalJ Immunol
PubMed ID8871630
'Post-translational modifications are important in regulating the functions of signal proteins. This is well established for intracellular proteins, but little is known in the case of extracellular domains of cell surface molecules. We recently described a cell surface protein, mono-ADP-ribosyltransferase (ADPRT), on cytotoxic T cells and showed that it mediates ... More
Muscarinic receptor-mediated dual regulation of ADP-ribosyl cyclase in NG108-15 neuronal cell membranes.
AuthorsHigashida H, Yokoyama S, Hashii M, Taketo M, Higashida M, Takayasu T, Ohshima T, Takasawa S, Okamoto H, Noda M
JournalJ Biol Chem
PubMed ID9395453
'Cyclic ADP-ribose (cADP-ribose) is an endogenous modulator of ryanodine-sensitive Ca2+ release channels. An unsolved question is whether or not cADP-ribose mediates intracellular signals from hormone or neurotransmitter receptors. The first step in this study was to develop a TLC method to measure ADP-ribosyl cyclase, by which conversion of [3H]NAD+ to ... More
Ca2+ entry induced by cyclic ADP-ribose in intact T-lymphocytes.
AuthorsGuse AH, Berg I, da Silva CP, Potter BV, Mayr GW
JournalJ Biol Chem
PubMed ID9079684
'Cyclic ADP-ribose (cADPr) is a potent Ca2+-mobilizing natural compound (Lee, H. C., Walseth, T. F., Bratt, G. T., Hayes, R. N., and Clapper, D. L. (1989) J. Biol. Chem. 264, 1608-1615) which has been shown to release Ca2+ from an intracellular store of permeabilized T-lymphocytes (Guse, A. H., Silva, C. ... More
Does the nuclear envelope contain two types of ligand-gated Ca2+ release channels?
AuthorsGuihard G, Proteau S, Rousseau E
JournalFEBS Lett
PubMed ID9305738
'The nuclear envelope is composed of two membranes deliminating a perinuclear space which displays functional properties similar to those of a Ca2+-storing compartment. ATP-driven Ca2+ uptake and InsP3-induced Ca2+ release processes have been described in isolated nuclei. Recently, it was reported that cADP-ribose and InsP3 can trigger a nucleoplasmic Ca2+ ... More
Paracrine roles of NAD+ and cyclic ADP-ribose in increasing intracellular calcium and enhancing cell proliferation of 3T3 fibroblasts.
AuthorsFranco L, Zocchi E, Usai C, Guida L, Bruzzone S, Costa A, De Flora A
JournalJ Biol Chem
PubMed ID11274199
'CD38 is a bifunctional ectoenzyme synthesizing from NAD(+) (ADP-ribosyl cyclase) and degrading (hydrolase) cyclic ADP-ribose (cADPR), a powerful universal calcium mobilizer from intracellular stores. Recently, hexameric connexin 43 (Cx43) hemichannels have been shown to release cytosolic NAD(+) from isolated murine fibroblasts (Bruzzone, S., Guida, L., Zocchi, E., Franco, L. and ... More
Polarized expression of Ca2+ channels in pancreatic and salivary gland cells. Correlation with initiation and propagation of [Ca2+]i waves.
AuthorsLee MG, Xu X, Zeng W, Diaz J, Wojcikiewicz RJ, Kuo TH, Wuytack F, Racymaekers L, Muallem S
JournalJ Biol Chem
PubMed ID9188472
'In polarized epithelial cells [Ca2+]i waves are initiated in discrete regions and propagate through the cytosol. The structural basis for these compartmentalized and coordinated events are not well understood. In the present study we used a combination of [Ca2+]i imaging at high temporal resolution, recording of Ca2+-activated Cl- current, and ... More
Regulation of calcium signalling in T lymphocytes by the second messenger cyclic ADP-ribose.
AuthorsGuse AH, da Silva CP, Berg I, Skapenko AL, Weber K, Heyer P, Hohenegger M, Ashamu GA, Schulze-Koops H, Potter BV, Mayr GW
JournalNature
PubMed ID10078531
'Cyclic ADP-ribose (cADPR) is a natural compound that mobilizes calcium ions in several eukaryotic cells. Although it can lead to the release of calcium ions in T lymphocytes, it has not been firmly established as a second messenger in these cells. Here, using high-performance liquid chromatography analysis, we show that ... More
Regulatory role of CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase) in insulin secretion by glucose in pancreatic beta cells. Enhanced insulin secretion in CD38-expressing transgenic mice.
AuthorsKato I, Takasawa S, Akabane A, Tanaka O, Abe H, Takamura T, Suzuki Y, Nata K, Yonekura H, Yoshimoto T
JournalJ Biol Chem
PubMed ID8530408
'Cyclic ADP-ribose (cADPR) serves as a second messenger for Ca2+ mobilization in insulin secretion, and CD38 has both ADP-ribosyl cyclase and cADPR hydrolase activities (Takasawa, S., Tohgo, A., Noguchi, N., Koguma, T., Nata, K., Sugimoto, T., Yonekura, H., and Okamoto, H. (1993) J. Biol. Chem. 268, 26052-26054). Here, we produced ... More
Adenine nucleotide diphosphates: emerging second messengers acting via intracellular Ca2+ release.
AuthorsDousa TP, Chini EN, Beers KW
JournalAm J Physiol
PubMed ID8897805
'Release of Ca2+ from intracellular stores is a widespread mechanism in regulation of cell function. Two hitherto unknown adenine diphosphonucleotides were recently identified, which trigger Ca2+ release from intracellular stores via channels that are distinct from the well-known receptor/channel controlled by inositol 1,4,5,-trisphosphate (IP3): cyclic ADP-ribose (cADPR) and nicotinic acid ... More
Cyclic ADP-ribose enhances coupling between voltage-gated Ca2+ entry and intracellular Ca2+ release.
AuthorsEmpson RM, Galione A
JournalJ Biol Chem
PubMed ID9261092
'Ca2+ release from intracellular stores can be activated in neurons by influx of Ca2+ through voltage-gated Ca2+ channels. This process, called Ca2+-induced Ca2+ release, relies on the properties of the ryanodine receptor and represents a mechanism by which Ca2+ influx during neuronal activity can be amplified into large intracellular Ca2+ ... More
The type 2 ryanodine receptor of neurosecretory PC12 cells is activated by cyclic ADP-ribose. Role of the nitric oxide/cGMP pathway.
AuthorsClementi E, Riccio M, Sciorati C, Nisticò G, Meldolesi J
JournalJ Biol Chem
PubMed ID8663443
'Of two neurosecretory PC12 cell clones that respond to NO donors and 8-bromo-cGMP with similar increases in cADP-ribose and that possess molecularly similar Ca2+ stores, only one (clone 16A) expresses the type 2 ryanodine receptor, whereas the other (clone 27) is devoid of ryanodine receptors. In PC12-16A cells, activation of ... More
CD38 disruption impairs glucose-induced increases in cyclic ADP-ribose, [Ca2+]i, and insulin secretion.
AuthorsKato I, Yamamoto Y, Fujimura M, Noguchi N, Takasawa S, Okamoto H
JournalJ Biol Chem
PubMed ID9890936
'Increases in [Ca2+]i in pancreatic beta cells, resulting from Ca2+ mobilization from intracellular stores as well as Ca2+ influx from extracellular sources, are important in insulin secretion by glucose. Cyclic ADP-ribose (cADPR), accumulated in beta cells by glucose stimulation, has been postulated to serve as a second messenger for intracellular ... More
Mechanistic implications of cyclic ADP-ribose hydrolysis and methanolysis catalyzed by calf spleen NAD+glycohydrolase.
AuthorsMuller-Steffner H, Muzard M, Oppenheimer N, Schuber F
JournalBiochem Biophys Res Commun
PubMed ID7980606
'Calf spleen NAD+glycohydrolase, besides its well known reactions, was shown to catalyze hydrolysis and methanolysis (with retention of configuration) of cyclic ADP-ribose, indicating that this classical NADase also belongs to the class of cyclic ADP-ribose hydrolases. No formation of cyclic ADP-ribose could be detected during the hydrolysis of beta-NAD+; moreover, ... More
Prolonged inactivation of nicotinic acid adenine dinucleotide phosphate-induced Ca2+ release mediates a spatiotemporal Ca2+ memory.
AuthorsChurchill GC, Galione A
JournalJ Biol Chem
PubMed ID11139579
'Although numerous extracellular stimuli are coupled to increases in intracellular Ca(2+), different stimuli are thought to achieve specificity by eliciting different spatiotemporal Ca(2+) increases. We investigated the effect of nicotinic acid adenine dinucleotide phosphate (NAADP) inactivation on spatiotemporal Ca(2+) signals in intact sea urchin eggs. The photorelease of NAADP but ... More
Cyclic ADP-ribose modulates Ca2+ release channels for activation by physiological Ca2+ entry in bullfrog sympathetic neurons.
AuthorsHua SY, Tokimasa T, Takasawa S, Furuya Y, Nohmi M, Okamoto H, Kuba K
JournalNeuron
PubMed ID8185944
'Although Ca(2+)-induced Ca2+ release (CICR) via ryanodine receptors has been found to occur in intact neurons, little is known about the physiological processes that regulate it. We studied the effects of cyclic ADP-ribose (cADPR) on CICR in cultured bullfrog sympathetic neurons by fura-2 fluorescence recording and patch-clamp techniques. cADPR applied ... More
Ca2+ and pancreatic B-cell function.
AuthorsBerggren PO, Larsson O
JournalBiochem Soc Trans
PubMed ID8206203
Cell signalling. A tale of two messengers.
AuthorsBerridge MJ
JournalNature
PubMed ID8413581
Characterization and application of photogeneration of calcium mobilizers cADP-ribose and nicotinic acid adenine dinucleotide phosphate from caged analogs.
AuthorsGee KR, Lee HC
JournalMethods Enzymol
PubMed ID9661161
Cyclic ADP-ribose: a new way to control calcium.
AuthorsGalione A
JournalScience
PubMed ID8380506
Preparation of cyclic ADP-ribose antagonists and caged cyclic ADP-ribose.
AuthorsWalseth TF, Aarhus R, Gurnack ME, Wong L, Breitinger HG, Gee KR, Lee HC
JournalMethods Enzymol
PubMed ID9211325
Cloning and characterization of cDNA encoding rat ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase (homologue to human CD38) from islets of Langerhans.
AuthorsKoguma T, Takasawa S, Tohgo A, Karasawa T, Furuya Y, Yonekura H, Okamoto H
JournalBiochim Biophys Acta
PubMed ID8061050
We report the cloning and cDNA sequence of rat CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase. Rat CD38 is composed of 303 amino acids and shares a high degree of homology with human and mouse CD38. Rat CD38 mRNA is expressed in various tissues including pancreatic islets but not in RINm5F cells. ... More
New aspects of the physiological significance of NAD, poly ADP-ribose and cyclic ADP-ribose.
AuthorsOkamoto H, Takasawa S, Tohgo A
JournalBiochimie
PubMed ID8527489
Cyclic ADP-ribose is generated from NAD+ in glucose-stimulated beta-cells by CD38. Cyclic ADP-ribose mobilizes Ca2+ from the endoplasmic reticulum to secrete insulin. The amino acid residues of Cys-119 and Cys-201 in CD38 are essential for the synthesis and hydrolysis of cyclic ADP-ribose. ... More
Cyclic ADP-ribose and calcium signaling in eggs.
AuthorsLee HC
JournalBiol Signals
PubMed ID8836493
Cyclic ADP-ribose is a cyclic nucleotide with Ca2+ signaling functions. It was first discovered in sea urchin eggs and has since been shown to be active in a variety of cells. Another Ca2+ release mechanism recently described in the eggs is mediated by nicotinic acid dinucleotide phosphate, a derivative of ... More
Abscisic acid signaling through cyclic ADP-ribose in plants.
AuthorsWu Y, Kuzma J, Maréchal E, Graeff R, Lee HC, Foster R, Chua NH
JournalScience
PubMed ID9405349
Abscisic acid (ABA) is the primary hormone that mediates plant responses to stresses such as cold, drought, and salinity. Single-cell microinjection experiments in tomato were used to identify possible intermediates involved in ABA signal transduction. Cyclic ADP-ribose (cADPR) was identified as a signaling molecule in the ABA response and was ... More
Calcium signaling by cyclic ADP-ribose and NAADP. A decade of exploration.
AuthorsLee HC
JournalCell Biochem Biophys
PubMed ID9386889
Ca2+ mobilization as a signaling mechanism has been placed on center stage with the discovery of the first Ca2+ messenger, inositol trisphosphate (IP3). This article focuses on two new Ca2+ release activators, which mobilize internal Ca2+ stores via mechanisms totally independent of IP3. They are cyclic ADP-ribose (cADPR) and nicotinic ... More
The versatility and universality of calcium signalling.
AuthorsBerridge MJ, Lipp P, Bootman MD
JournalNat Rev Mol Cell Biol
PubMed ID11413485
The universality of calcium as an intracellular messenger depends on its enormous versatility. Cells have a calcium signalling toolkit with many components that can be mixed and matched to create a wide range of spatial and temporal signals. This versatility is exploited to control processes as diverse as fertilization, proliferation, ... More
ADP-ribosyl cyclase coupled with receptors via G proteins.
AuthorsHigashida H
JournalFEBS Lett
PubMed ID9428744
Crude cell membranes in mammalian cells contain ADP-ribosyl cyclase, which converts NAD+ to cyclic ADP-ribose. Acetylcholine either increases or inhibits this activity in NG108-15 neuronal cells and adrenal chromaffin cells in a muscarinic receptor subtype-specific manner. Activation or inhibition of the cyclase activity is mimicked by GTP and blocked by ... More
Inhibition of cADPR-Hydrolase by ADP-ribose potentiates cADPR synthesis from beta-NAD+.
AuthorsGenazzani AA, Bak J, Galione A
JournalBiochem Biophys Res Commun
PubMed ID8687425
Cyclic adenosine 5'-diphosphate ribose (cADPR) is a potent Ca2+ releasing agent in a number of tissues. A particular bifunctional NAD+ glycohydrolase is responsible for both the cyclase and hydrolase activity necessary for its synthesis from beta-NAD and degradation to ADPR. We now report that ADPR, the end-product of this enzyme, ... More
Cyclic ADP-ribose: a new member of a super family of signalling cyclic nucleotides.
AuthorsLee HC
JournalCell Signal
PubMed ID7857763
Cyclic nucleotides are second messengers exerting their cellular effects mainly through protein phosphorylation. A new member of this family, cyclic ADP-ribose, is involved, instead, in mediating mobilization of Ca+2 from internal stores. The structure of this nucleotide has now been determined by X-ray crystallography and accumulating evidence indicates it may ... More
Cyclic ADP-ribose: a calcium mobilizing metabolite of NAD+.
AuthorsLee HC
JournalMol Cell Biochem
PubMed ID7898468
Mobilization of Ca+2 from intracellular stores is a signalling mechanism that is of fundamental importance to many cellular processes. It is mediated by two major mechanisms, the inositol 1,4,5-trisphosphate pathway and the Ca+2-induced Ca+2 release process. A naturally occurring metabolite of NAD+ called cyclic ADP-ribose has been discovered recently and ... More
AuthorsMorrissette J, Heisermann G, Cleary J, Ruoho A, Coronado R
JournalFEBS Lett
PubMed ID8375498
The Ca(2+)-mobilizing metabolite cyclic ADP-ribose (cADPR) has been shown to release Ca2+ from ryanodine-sensitive stores in many cells. We show that this metabolite at a concentration of 17 microM, but not its precursor beta-NAD+ nor non-cyclic ADPR at the same concentration, is active in releasing Ca2+ from rabbit skeletal muscle ... More
Release of Ca2+ from individual plant vacuoles by both InsP3 and cyclic ADP-ribose.
AuthorsAllen GJ, Muir SR, Sanders D
JournalScience
PubMed ID7732384
Calcium mobilization from intracellular pools couples many stimuli to responses in plant cells. Cyclic adenosine 5'-diphosphoribose (cADPR), which interacts with a ryanodine receptor in certain animal cells, was shown to elicit calcium release at the vacuolar membrane of beet storage root. The vacuolar calcium release pathway showed similarities to cADPR-gated ... More
Magnesium ions but not ATP inhibit cyclic ADP-ribose-induced calcium release.
AuthorsGraeff RM, Podein RJ, Aarhus R, Lee HC
JournalBiochem Biophys Res Commun
PubMed ID7826401
The pharmacology of the cyclic ADP-ribose (cADPR)-dependent Ca2+ release mechanism is very similar to that of the ryanodine receptor (RyR). Here we showed that MgCl2, a known inhibitor of RyR, blocked cADPR-induced Ca+2 release in sea urchin egg homogenates with a half maximal concentration of about 2.5 mM. The effect ... More
Bioorganic chemistry of cyclic ADP-ribose (cADPR).
AuthorsZhang FJ, Gu QM, Sih CJ
JournalBioorg Med Chem
PubMed ID10400320
The objective of this brief review is to present an overview of the bioorganic chemistry of cyclic-ADP-ribose (cADPR) with special emphasis on the methodology used for the synthesis of analogues of cADPR. New structural analogues of cADPR can be prepared using either the biomimetic method or ADP-ribosyl cyclase from Aplysia ... More
Calcium mobilization by dual receptors during fertilization of sea urchin eggs.
AuthorsLee HC, Aarhus R, Walseth TF
JournalScience
PubMed ID8392749
Fertilization is accompanied by a transient increase in the concentration of intracellular Ca2+, which serves as a signal for initiating development. Some of the Ca2+ appears to be released from intracellular stores by the binding of inositol trisphosphate (IP3) to its receptor. However, in sea urchin eggs, other mechanisms appear ... More
ADP-ribosyl cyclase and CD38 catalyze the synthesis of a calcium- mobilizing metabolite from NADP.
AuthorsAarhus R, Graeff RM, Dickey DM, Walseth TF, Lee HC
JournalJ Biol Chem
PubMed ID8530456
ADP-ribosyl cyclase catalyzes the cyclization of NAD+ to produce cyclic ADP-ribose (cADPR), which is emerging as an endogenous regulator of the Ca(2+)-induced Ca2+ release mechanism in cells. CD38 is a lymphocyte differentiation antigen which has recently been shown to be a bifunctional enzyme that can synthesize cADPR from NAD+ as ... More
Cyclic ADP-ribose regulation of ryanodine receptors involved in agonist evoked cytosolic Ca2+ oscillations in pancreatic acinar cells.
AuthorsThorn P, Gerasimenko O, Petersen OH
JournalEMBO J
PubMed ID7514529
We have investigated the role of the ryanodine-sensitive intracellular Ca2+ release channel (ryanodine receptor) in the cytosolic Ca2+ oscillations evoked in pancreatic acinar cells by acetylcholine (ACh) or cholecystokinin (CCK). Ryanodine abolished or markedly inhibited the agonist evoked Ca2+ spiking, but enhanced the frequency of spikes evoked by direct internal ... More
Unique inactivation properties of NAADP-sensitive Ca2+ release.
AuthorsGenazzani AA, Empson RM, Galione A
JournalJ Biol Chem
PubMed ID8662773
Ca2+ mobilization from intracellular stores constitutes an important mechanism for generating cytoplasmic Ca2+ signals. Inositol trisphosphate (InsP3) and ryanodine receptors are the two families of intracellular Ca2+ release channels that have been identified, which may be regulated by separate intracellular messengers, InsP3, and cyclic adenosine 5'-diphosphate ribose, respectively. A third ... More
Cyclic ADP ribose activation of the ryanodine receptor is mediated by calmodulin.
Cyclic ADP-ribose (cADPR) is a newly identified nucleotide which can release calcium from a variety of cells, suggesting it is a messenger for mobilizing internal Ca2+ stores. Its cyclic structure has now been confirmed by X-ray crystallography. Available results are consistent with it being a modulator of Ca(2+)-induced Ca2+ release. ... More
Adenosine 5'-cyclic diphosphoribose [cyclic ADP-ribose (cADPR)], a metabolite of NAD+ that promotes Ca2+ release from sea urchin egg homogenates and microsomal fractions, has been proposed to act as an endogenous agonist of Ca2+ release in sea urchin eggs. We describe experiments showing that a microsomal fraction isolated from Tetrapigus nyger ... More
Cyclic ADP-ribose as an endogenous regulator of the non-skeletal type ryanodine receptor Ca2+ channel.
AuthorsMészáros LG, Bak J, Chu A
JournalNature
PubMed ID8391127
The skeletal and cardiac isoforms of the ryanodine receptor Ca2+ channel (RyRC) constitute the Ca2+ release pathway in sarcoplasmic reticulum of skeletal and cardiac muscles, respectively. A direct mechanical and a Ca(2+)-triggered mechanism (Ca(2+)-induced Ca2+ release) have been respectively proposed to explain the in situ activation of Ca2+ release in ... More
Differential regulation of nicotinic acid-adenine dinucleotide phosphate and cADP-ribose production by cAMP and cGMP.
AuthorsWilson HL, Galione A
JournalBiochem J
PubMed ID9560312
The sea urchin egg has been used as a system to study calcium-release mechanisms induced by inositol 1,4,5-trisphosphate (IP3), cADP-ribose (cADPR), and more recently, nicotinic acid-adenine dinucleotide phosphate (NAADP). In order that cADPR and NAADP may be established as endogenous messengers for calcium release, the existence of intracellular enzymes capable ... More
Nicotinate-adenine dinucleotide phosphate-induced Ca(2+)-release does not behave as a Ca(2+)-induced Ca(2+)-release system.
AuthorsChini EN, Dousa TP
JournalBiochem J
PubMed ID8670142
We investigated the dependence of nicotinate-adenine dinucleotide phosphate (NAADP)-induced Ca2+ release from intracellular stores of sea urchin egg homogenates, upon extravesicular Ca2+. In contrast to the Ca2+ release induced inositol 1',4',5'-triphosphate (IP3) or cyclic ADP-ribose (cADPR), the Ca2+ release induced by NAADP was completely independent of the free extravesicular Ca2+ ... More
Calcium dynamics in the central nervous system.
AuthorsDeCoster MA
JournalAdv Neuroimmunol
PubMed ID8748068
Calcium ions are critically important in many functions of the nervous system from neurotransmitter release to intracellular signal transduction. The large difference between intracellular and extracellular calcium ion concentration ([Ca2+]) highlights the importance of the mechanisms controlling influx and efflux of this ion. Loss of the regulatory ability of these ... More
Calcium signalling in and around the nuclear envelope.
AuthorsGerasimenko J, Maruyama Y, Tepikin A, Petersen OH, Gerasimenko O
JournalBiochem Soc Trans
PubMed ID12546657
We have compared calcium mobilization by Ins(1,4,5) P(3)(IP(3)), cADP-ribose (cADPR) and nicotinic acid-adenosine dinucleotide phosphate (NAADP) from the envelope of isolated nuclei with the calcium signalling in intact isolated pancreatic acinar cells. Ca(2+) uptake and release were studied with calcium-sensitive fluorescent probes. In the present study, we have shown that ... More
Coordination of agonist-induced Ca2+-signalling patterns by NAADP in pancreatic acinar cells.
AuthorsCancela JM, Churchill GC, Galione A
JournalNature
PubMed ID10078532
Many hormones and neurotransmitters evoke Ca2+ release from intracellular stores, often triggering agonist-specific signatures of intracellular Ca2+ concentration. Inositol trisphosphate (InsP3) and cyclic adenosine 5'-diphosphate-ribose (cADPR) are established Ca2+-mobilizing messengers that activate Ca2+ release through intracellular InsP3 and ryanodine receptors, respectively. However, in pancreatic acinar cells, neither messenger can explain ... More
ATP-dependent accumulation and inositol trisphosphate- or cyclic ADP-ribose-mediated release of Ca2+ from the nuclear envelope.
Uptake and release of Ca2+ from isolated liver nuclei were studied with fluorescent probes. We show with the help of digital imaging and confocal microscopy that the Ca(2+)-sensitive fluorescent probe Fura 2 is concentrated in or around the nuclear envelope and that the distribution of Fura 2 fluorescence is similar ... More
Requirement of calmodulin-dependent protein kinase II in cyclic ADP-ribose-mediated intracellular Ca2+ mobilization.
AuthorsTakasawa S, Ishida A, Nata K, Nakagawa K, Noguchi N, Tohgo A, Kato I, Yonekura H, Fujisawa H, Okamoto H
JournalJ Biol Chem
PubMed ID8530441
Cyclic ADP-ribose (cADPR) is generated in pancreatic islets by glucose stimulation, serving as a second messenger for Ca2+ mobilization from the endoplasmic reticulum for insulin secretion (Takasawa, S., Nata, K., Yonekura, H., and Okamoto, H. (1993) Science 259, 370-373). In the present study, we observed that the addition of calmodulin ... More
Cyclic ADP-ribose competes with ATP for the adenine nucleotide binding site on the cardiac ryanodine receptor Ca(2+)-release channel.
AuthorsSitsapesan R, McGarry SJ, Williams AJ
JournalCirc Res
PubMed ID8062431
We have investigated the mechanism of action of the putative second messenger, cyclic ADP-ribose (cADPR), on the cardiac ryanodine-sensitive Ca(2+)-release channel. Current fluctuations through single Ca(2+)-release channels have been monitored after incorporation into planar phospholipid bilayers. We demonstrate that activation of the channel by cADPR is dependent on activating levels ... More
Sympathetic potentiation of cyclic ADP-ribose formation in rat cardiac myocytes.
AuthorsHigashida H, Egorova A, Higashida C, Zhong ZG, Yokoyama S, Noda M, Zhang JS
JournalJ Biol Chem
PubMed ID10559213
We examined the role of cyclic ADP-ribose (cADP-ribose) as a second messenger downstream of adrenergic receptors in the heart after excitation of sympathetic neurons. To address this question, ADP-ribosyl cyclase activity was measured as the rate of [(3)H]cADP-ribose formation from [(3)H]NAD(+) in a crude membrane fraction of rat ventricular myocytes. ... More
Essential cysteine residues for cyclic ADP-ribose synthesis and hydrolysis by CD38.
AuthorsTohgo A, Takasawa S, Noguchi N, Koguma T, Nata K, Sugimoto T, Furuya Y, Yonekura H, Okamoto H
JournalJ Biol Chem
PubMed ID7961800
We have recently demonstrated that cyclic ADP-ribose (cADPR) serves as a second messenger for glucose-induced insulin secretion (Takasawa, S., Nata, K., Yonekura, H., and Okamoto, H. (1993) Science 259, 370-373) and that human leukocyte antigen CD38 has both ADP-ribosyl cyclase and cADPR hydrolase activities (Takasawa, S., Tohgo, A., Noguchi, N., ... More
Lysine 129 of CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase) participates in the binding of ATP to inhibit the cyclic ADP-ribose hydrolase.
AuthorsTohgo A, Munakata H, Takasawa S, Nata K, Akiyama T, Hayashi N, Okamoto H
JournalJ Biol Chem
PubMed ID9020087
CD38 catalyzes not only the formation of cyclic ADP-ribose (cADPR) from NAD+ but also the hydrolysis of cADPR to ADP-ribose (ADPR), and ATP inhibits the hydrolysis (Takasawa, S., Tohgo, A., Noguchi, N., Koguma, T., Nata, K., Sugimoto, T., Yonekura, H., and Okamoto, H. (1993) J. Biol. Chem. 268, 26052-26054). In ... More
Caged cyclic ADP-ribose. Synthesis and use.
AuthorsAarhus R, Gee K, Lee HC
JournalJ Biol Chem
PubMed ID7706323
Cyclic ADP-ribose (cADPR) is a recently discovered cyclic nucleotide with Ca2+ mobilizing activity. Caged cADPR was synthesized by reacting cADPR with 2-nitrophenethyldiazoethane. Elemental analyses, 1H NMR, and extinction coefficient measurements indicate that the product contains only one caging group. Anion exchange high pressure liquid chromatography separated caged cADPR into two ... More
Novel mechanism of intracellular calcium release in pituitary cells.
AuthorsKoshiyama H, Lee HC, Tashjian AH
JournalJ Biol Chem
PubMed ID1894597
In sea urchin eggs an enzymatic metabolite of beta-NAD+, called cyclic ADP-ribose (cADPR), is as potent and powerful a releaser of sequestered intracellular Ca2+ as is inositol 1,4,5-trisphosphate (IP3). The enzyme that synthesizes cADPR is present in several vertebrate animal tissues, but the Ca(2+)-releasing activity of cADPR has not been ... More
Cyclic ADP-ribose and inositol 1,4,5-trisphosphate as alternate second messengers for intracellular Ca2+ mobilization in normal and diabetic beta-cells.
AuthorsTakasawa S, Akiyama T, Nata K, Kuroki M, Tohgo A, Noguchi N, Kobayashi S, Kato I, Katada T, Okamoto H
JournalJ Biol Chem
PubMed ID9446548
Intracellular Ca2+ mobilization occurs in a variety of cellular processes and is mediated by two major systems, the inositol 1,4, 5-trisphosphate (IP3) and cyclic ADP-ribose (cADPR) systems. cADPR has been proposed to be a second messenger for insulin secretion induced by glucose in pancreatic beta-cells (Takasawa, S., Nata, K., Yonekura, ... More
Modulation of renal Na-Pi cotransport by hormones acting via genomic mechanism and by metabolic factors.
AuthorsDousa TP
JournalKidney Int
PubMed ID8691752
The renal Na-Pi cotransport is subject to multiple regulatory inputs, such as endocrine, paracrine and intracrine. Among lipophilic, long-acting hormones that act via genomic mechanism, thyroid hormones, calcitriol, all-trans-retinoic acid stimulate, whereas glucocorticoids and estradiol inhibit the rate of Na-Pi cotransport across the brush border membrane of proximal tubules in ... More
Role of cyclic ADP-ribose in the regulation of [Ca2+]i in porcine tracheal smooth muscle.
The purpose of the present study was to determine whether cyclic ADP-ribose (cADPR) acts as a second messenger for Ca2+ release through ryanodine receptor (RyR) channels in tracheal smooth muscle (TSM). Freshly dissociated porcine TSM cells were permeabilized with beta-escin, and real-time confocal microscopy was used to examine changes in ... More
Expression of CD38 increases intracellular calcium concentration and reduces doubling time in HeLa and 3T3 cells.
AuthorsZocchi E, Daga A, Usai C, Franco L, Guida L, Bruzzone S, Costa A, Marchetti C, De Flora A
JournalJ Biol Chem
PubMed ID9525901
CD38 is a bifunctional ectoenzyme, predominantly expressed on hematopoietic cells during differentiation, that catalyzes the synthesis (cyclase) and the degradation (hydrolase) of cyclic ADP-ribose (cADPR), a powerful calcium mobilizer from intracellular stores. Due to the well established role of calcium levels in the regulation of apoptosis, proliferation, and differentiation, the ... More
Glycation and glycoxidation of histones by ADP-ribose.
AuthorsCervantes-Laurean D, Jacobson EL, Jacobson MK
JournalJ Biol Chem
PubMed ID8631841
The reaction of long lived proteins with reducing sugars has been implicated in the pathophysiology of aging and age-related diseases. A likely intranuclear source of reducing sugar is ADP-ribose, which is generated following DNA damage from the turnover of ADP-ribose polymers. In this study, ADP-ribose has been shown to be ... More
Wide distribution of an enzyme that catalyzes the hydrolysis of cyclic ADP-ribose.
AuthorsLee HC, Aarhus R
JournalBiochim Biophys Acta
PubMed ID8518298
Cyclic ADP-ribose (cADPR) is a metabolite of NAD+ that is as effective as inositol trisphosphate in mobilizing intracellular-Ca2+ stores. Its synthesizing enzyme, ADP-ribosyl cyclase, has been shown to be present in mammalian and invertebrate tissues. In this study we identify another widely-distributed enzyme that can hydrolyze cADPR to ADP-ribose. Incubation ... More
A cytosolic sperm protein factor mobilizes Ca2+ from intracellular stores by activating multiple Ca2+ release mechanisms independently of low molecular weight messengers.
AuthorsGalione A, Jones KT, Lai FA, Swann K
JournalJ Biol Chem
PubMed ID9360959
Ca2+ oscillations can be induced in mammalian eggs and somatic cells by microinjection of a cytosolic sperm protein factor. The nature of the sperm factor-induced Ca2+ signaling was investigated by adding sperm protein extracts to homogenates of sea urchin eggs, which contain multiple classes of Ca2+ release mechanisms. We show ... More
Ca2+-induced Ca2+ release in chromaffin cells seen from inside the ER with targeted aequorin.
AuthorsAlonso MT, Barrero MJ, Michelena P, Carnicero E, Cuchillo I, García AG, García-Sancho J, Montero M, Alvarez J
JournalJ Cell Biol
PubMed ID9922451
The presence and physiological role of Ca2+-induced Ca2+ release (CICR) in nonmuscle excitable cells has been investigated only indirectly through measurements of cytosolic [Ca2+] ([Ca2+]c). Using targeted aequorin, we have directly monitored [Ca2+] changes inside the ER ([Ca2+]ER) in bovine adrenal chromaffin cells. Ca2+ entry induced by cell depolarization triggered ... More
Interaction of two classes of ADP-ribose transfer reactions in immune signaling.
AuthorsHan MK, Cho YS, Kim YS, Yim CY, Kim UH
JournalJ Biol Chem
PubMed ID10777496
CD38 is a bifunctional ectoenzyme predominantly expressed on hematopoietic cells where its expression correlates with differentiation and proliferation. The two enzyme activities displayed by CD38 are an ADP-ribosyl cyclase and a cyclic adenosine diphosphate ribose (cADPR) hydrolase that catalyzes the synthesis and hydrolysis of cADPR. T lymphocytes can be induced ... More
Identification of cyclic ADP-ribose-dependent mechanisms in pancreatic muscarinic Ca(2+) signaling using CD38 knockout mice.
AuthorsFukushi Y, Kato I, Takasawa S, Sasaki T, Ong BH, Sato M, Ohsaga A, Sato K, Shirato K, Okamoto H, Maruyama Y
JournalJ Biol Chem
PubMed ID11001947
We showed that muscarinic acetylcholine (ACh)-stimulation increased the cellular content of cADPR in the pancreatic acinar cells from normal mice but not in those from CD38 knockout mice. By monitoring ACh-evoked increases in the cytosolic Ca(2+) concentration ([Ca(2+)](i)) using fura-2 microfluorimetry, we distinguished and characterized the Ca(2+) release mechanisms responsive ... More
Stimulation of cyclic ADP-ribose synthesis by acetylcholine and its role in catecholamine release in bovine adrenal chromaffin cells.
AuthorsMorita K, Kitayama S, Dohi T
JournalJ Biol Chem
PubMed ID9261101
Cyclic ADP-ribose (cADPR) is suggested to be a novel messenger of ryanodine receptors in various cellular systems. However, the regulation of its synthesis in response to cell stimulation and its functional roles are still unclear. We examined the physiological relevance of cADPR to the messenger role in stimulation-secretion coupling in ... More
Insulin exocytosis and glucose-mediated increase in cytoplasmic free Ca2+ concentration in the pancreatic beta-cell are independent of cyclic ADP-ribose.
AuthorsWebb DL, Islam MS, Efanov AM, Brown G, Köhler M, Larsson O, Berggren PO
JournalJ Biol Chem
PubMed ID8702579
Stimulation of pancreatic beta-cells by glucose gives rise to an increase in the cytoplasmic free calcium concentration ([Ca2+]i) and exocytosis of insulin. Cyclic adenosine 5'-diphosphate ribose (cADPR), a metabolite of beta-NAD+, has been reported to increase [Ca2+]i in pancreatic beta-cells by releasing Ca2+ from inositol 1,4,5-trisphosphate-insensitive intracellular stores. In the ... More
Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus.
AuthorsKhoo KM, Han MK, Park JB, Chae SW, Kim UH, Lee HC, Bay BH, Chang CF
JournalJ Biol Chem
PubMed ID10818108
CD38 is a type II transmembrane glycoprotein found on both hematopoietic and non-hematopoietic cells. It is known for its involvement in the metabolism of cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate, two nucleotides with calcium mobilizing activity independent of inositol trisphosphate. It is generally believed that CD38 is ... More
Kinetic properties of nicotinic acid adenine dinucleotide phosphate-induced Ca2+ release.
AuthorsGenazzani AA, Mezna M, Summerhill RJ, Galione A, Michelangeli F
JournalJ Biol Chem
PubMed ID9065423
Three endogenous molecules have now been shown to release Ca2+ in the sea urchin egg: inositol trisphosphate (InsP3), cyclic adenosine 5'-diphosphate ribose (cADPR), and nicotinic acid adenine dinucleotide phosphate (NAADP), a derivative of NADP. While the mechanism through which the first two molecules are able to release Ca2+ is established ... More
Sarcoplasmic reticulum-associated and protein kinase C-regulated ADP-ribosyl cyclase in cardiac muscle.
AuthorsMészáros LG, Wrenn RW, Váradi G
JournalBiochem Biophys Res Commun
PubMed ID9168998
Two types of ADP-ribosyl cyclase activity were distinguished in dog and rat cardiac muscles by measuring the enzymatic conversion of NGD (as an NAD analog) into the fluorescent product cyclic GDP-ribose in cardiac muscle subcellular fractions. Both types of activity were confined to membrane fractions isolated from microsomes by sucrose ... More
Cyclic 3-deaza-adenosine diphosphoribose: a potent and stable analog of cyclic ADP-ribose.
AuthorsWong L, Aarhus R, Lee HC, Walseth TF
JournalBiochim Biophys Acta
PubMed ID10564770
Cyclic 3-deaza-adenosine diphosphoribose (3-deaza-cADPR), an analog of cyclic adenosine diphosphoribose (cADPR) was synthesized. 3-deaza-cADPR differs from cADPR by only the substitution of carbon for nitrogen at the 3-position of the purine ring. Similar to cADPR, the analog has potent calcium releasing activity in sea urchin egg homogenates and was able ... More
Adp-ribosyl cyclase and cyclic ADP-ribose hydrolase act as a redox sensor. a primary role for cyclic ADP-ribose in hypoxic pulmonary vasoconstriction.
AuthorsWilson HL, Dipp M, Thomas JM, Lad C, Galione A, Evans AM
JournalJ Biol Chem
PubMed ID11116136
Hypoxic pulmonary vasoconstriction is unique to pulmonary arteries and serves to match lung perfusion to ventilation. However, in disease states this process can promote hypoxic pulmonary hypertension. Hypoxic pulmonary vasoconstriction is associated with increased NADH levels in pulmonary artery smooth muscle and with intracellular Ca(2+) release from ryanodine-sensitive stores. Because ... More
7-Deaza-8-bromo-cyclic ADP-ribose, the first membrane-permeant, hydrolysis-resistant cyclic ADP-ribose antagonist.
AuthorsSethi JK, Empson RM, Bailey VC, Potter BV, Galione A
JournalJ Biol Chem
PubMed ID9195942
Cyclic ADP-ribose (cADPR) is a putative second messenger that has been demonstrated to mobilize Ca2+ in many cell types. Its postulated role as the endogenous regulator of ryanodine-sensitive Ca2+ release channels has been greatly supported by the advent and use of specific cADPR receptor antagonists such as 8-NH2-cADPR (Walseth, T. ... More