A beta-catenin survival signal is required for normal lobular development in the mammary gland.
AuthorsTepera SB, McCrea PD, Rosen JM,
JournalJ Cell Sci
PubMed ID12584256
'The Wnt (wingless) family of secreted glycoproteins initiates a signalling pathway implicated in the regulation of both normal mouse mammary gland development and tumorigenesis. Multiple Wnt signals ultimately converge on the multifunctional protein beta-catenin to activate the transcription of target genes. Although beta-catenin plays a crucial role in canonical Wnt ... More
Evidence that fertilization activates starfish eggs by sequential activation of a Src-like kinase and phospholipase cgamma.
AuthorsGiusti AF, Xu W, Hinkle B, Terasaki M, Jaffe LA
JournalJ Biol Chem
PubMed ID10747984
'Recent evidence has indicated a requirement for a Src family kinase in initiating Ca(2+) release at fertilization in starfish eggs (Giusti, A. F., Carroll, D. J., Abassi, Y. A., Terasaki, M., Foltz, K. R., and Jaffe, L. A. (1999) J. Biol. Chem. 274, 29318-29322). We now show that injection of ... More
High-density polymerase-mediated incorporation of fluorochrome-labeled nucleotides.
AuthorsRamanathan A, Pape L, Schwartz DC
JournalAnal Biochem
PubMed ID15649370
'DNA-polymerase-mediated incorporation of different fluorochrome-labeled nucleotides (FdNTPs) was investigated with the goals of optimizing the high-density labeling of probes and exploring DNA sequencing strategies that rely on the controlled, sequential addition of such compounds. By systematically evaluating variables--including polymerase type, buffer conditions, and fluorochrome chemistries--a rational strategy for the sequential ... More
Identification of PLCgamma-dependent and -independent events during fertilization of sea urchin eggs.
AuthorsCarroll DJ, Albay DT, Terasaki M, Jaffe LA, Foltz KR
JournalDev Biol
PubMed ID9986735
At fertilization, sea urchin eggs undergo a series of activation events, including a Ca2+ action potential, Ca2+ release from the endoplasmic reticulum, an increase in intracellular pH, sperm pronuclear formation, MAP kinase dephosphorylation, and DNA synthesis. To examine which of these events might be initiated by activation of phospholipase Cgamma ... More