Endogenous endothelial cell nitric-oxide synthase modulates apoptosis in cultured breast cancer cells and is transcriptionally regulated by p53.
AuthorsMortensen K,Skouv J,Hougaard DM,Larsson LI
JournalThe Journal of biological chemistry
PubMed ID10608825
Role of thiols in the targeting of S-nitroso thiols to red blood cells.
AuthorsPietraforte D, Mallozzi C, Scorza G, Minetti M
JournalBiochemistry
PubMed ID7766628
We compared the nitric oxide (.NO)-releasing characteristics of two NO donors, the S-nitroso adduct of bovine serum albumin (BSANO) and the S-nitroso adduct of L-glutathione (GSNO). In oxygenated phosphate buffer (pH 7.4) and in hemoglobin solution, both NO donors released .NO only in the presence of a low molecular weight ... More
Arginase II downregulates nitric oxide (NO) production and prevents NO-mediated apoptosis in murine macrophage-derived RAW 264.7 cells.
AuthorsGotoh T, Mori M
JournalJ Cell Biol
PubMed ID9971738
'Excess nitric oxide (NO) induces apoptosis of some cell types, including macrophages. As NO is synthesized by NO synthase (NOS) from arginine, a common substrate of arginase, these two enzymes compete for arginine. There are two known isoforms of arginase, types I and II. Using murine macrophage-like RAW 264.7 cells, ... More
Antagonistic action of imidazolineoxyl N-oxides against endothelium-derived relaxing factor/.NO through a radical reaction.
AuthorsAkaike T, Yoshida M, Miyamoto Y, Sato K, Kohno M, Sasamoto K, Miyazaki K, Ueda S, Maeda H
JournalBiochemistry
PubMed ID8422387
'A labile inorganic free radical, nitric oxide (.NO), is produced by nitric oxide synthase from the substrate L-arginine in various cells and tissues. It acts as an endothelium-derived relaxing factor (EDRF) or as a neurotransmitter in vivo. We investigated the reactivity of stable radical compounds, imidazolineoxyl N-oxides such as 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl ... More
In vivo imaging of an elicitor-induced nitric oxide burst in tobacco.
AuthorsFoissner I, Wendehenne D, Langebartels C, Durner J
JournalPlant J
PubMed ID10998192
'A growing body of evidence suggests that nitric oxide (NO), an important signalling and defence molecule in mammals, plays a key role in activating disease resistance in plants, acting as signalling molecule and possibly as direct anti-microbial agent. Recently, a novel fluorophore (diaminofluorescein diacetate, DAF-2 DA) has been developed which ... More
Rapid attenuation of AP-1 transcriptional factors associated with nitric oxide (NO)-mediated neuronal cell death.
AuthorsTabuchi A, Oh E, Taoka A, Sakurai H, Tsuchiya T, Tsuda M
JournalJ Biol Chem
PubMed ID8940101
'Stimulation of glutamate receptors causes several intracellular reactions including activation of activator protein-1 (AP-1) production and nitric oxide (NO) generation. Exposing mouse cerebellar granule cells to N-methyl-D-aspartate or kainate (KA) in culture induced an increase of AP-1 DNA binding activity that was blocked by further addition of sodium nitroprusside (SNP), ... More
Nitric oxide modulates a late step of exocytosis.
AuthorsMachado JD, Segura F, Brioso MA, Borges R
JournalJ Biol Chem
PubMed ID10747967
'The effects of nitric oxide (NO) on the late phase of exocytosis have been studied, by amperometry, on Ba(2+)-stimulated chromaffin cells. Acute incubation with NO or NO donors (sodium nitroprusside, spermine-NO, S-nitrosoglutathione) produced a drastic slowdown of the granule emptying. Conversely, cell treatment with N(omega)-nitro-l-arginine methyl ester (a NO synthase ... More
'The source of superoxide (O2*-) production and cell-to-cell interactions of O2*- and nitric oxide (NO) in response to angiotensin II (AngII) were studied by fluorescence microscopic techniques to image rat renal outer medullary microtissue strips. Changes in intracellular O2*- were determined by dihydroethidium-ethidium ratios, and NO was determined with 4,5-diaminofluorescein ... More
Hydrogen peroxide is an endothelium-derived hyperpolarizing factor in mice.
AuthorsMatoba T, Shimokawa H, Nakashima M, Hirakawa Y, Mukai Y, Hirano K, Kanaide H, Takeshita A
JournalJ Clin Invest
PubMed ID11120759
'The endothelium plays an important role in maintaining vascular homeostasis by synthesizing and releasing several endothelium-derived relaxing factors, such as prostacyclin, nitric oxide (NO), and the previously unidentified endothelium-derived hyperpolarizing factor (EDHF). In this study, we examined our hypothesis that hydrogen peroxide (H(2)O(2)) derived from endothelial NO synthase (eNOS) is ... More
Improved detection of nitric oxide radical (NO.) production in an activated macrophage culture with a radical scavenger, carboxy PTIO and Griess reagent.
AuthorsAmano F, Noda T
JournalFEBS Lett
PubMed ID7543422
'An improved method for the detection of nitric oxide radicals (NO. in cultures of activated macrophages was developed, involving a nitric oxide radical scavenger, 2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-3-oxide-1-oxyl (carboxy PTIO) and Griess reagent. A murine macrophage-like cell line, J774.1, was activated with interferon-gamma (IFN-gamma) and bacterial lipopolysaccharide (LPS), which induced the production ... More
Inducible nitric-oxide synthase attenuates vasopressin-dependent Ca2+ signaling in rat hepatocytes.
Authors Patel Sandip; Gaspers Lawrence D; Boucherie Sylviane; Memin Elisabeth; Stellato Kerri Anne; Guillon Gilles; Combettes Laurent; Thomas Andrew P;
JournalJ Biol Chem
PubMed ID12097323
'Increases in both Ca(2+) and nitric oxide levels are vital for a variety of cellular processes; however, the interaction between these two crucial messengers is not fully understood. Here, we demonstrate that expression of inducible nitric-oxide synthase in hepatocytes, in response to inflammatory mediators, dramatically attenuates Ca(2+) signaling by the ... More
Ultraviolet B radiation acts through the nitric oxide and cGMP signal transduction pathway to stimulate melanogenesis in human melanocytes.
AuthorsRoméro-Graillet C, Aberdam E, Biagoli N, Massabni W, Ortonne JP, Ballotti R
JournalJ Biol Chem
PubMed ID8910416
'Ultraviolet B (UVB) radiation is the main physiological stimulus for human skin pigmentation; however, the molecular mechanisms underlying this process are still unclear. Recently, nitric oxide (NO) and cGMP have been involved in mediation of skin erythema induced by UVB. Therefore, we investigated the role of NO and cGMP in ... More
Modulation of airway smooth muscle beta-adrenoceptor function by a muscarinic agonist.
AuthorsGrandordy BM, Mak JC, Barnes PJ
JournalLife Sci
PubMed ID7904709
'We have investigated the effect of a muscarinic agonist and protein kinase C (PKC) activation on beta-adrenoceptors and their coupling to adenylyl cyclase in bovine tracheal smooth muscle. There was a significant reduction in maximum binding capacity of [125I]iodocyanopindolol ([125I]ICYP) after exposure to carbachol and 4 beta-phorbol 12 beta-myristate 13 ... More
Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration.
Protection from NO gas, a toxic byproduct of anaerobic respiration in Pseudomonas aeruginosa, is mediated by nitric oxide (NO) reductase (NOR), the norCB gene product. Nevertheless, a norCB mutant that accumulated approximately 13.6 microM NO paradoxically survived anaerobic growth. Transcription of genes encoding nitrate and nitrite reductases, the enzymes responsible ... More
Trapping of nitric oxide formed during photolysis of sodium nitroprusside in aqueous and lipid phases: an electron spin resonance study.
AuthorsSingh RJ, Hogg N, Neese F, Joseph J, Kalyanaraman B
JournalPhotochem Photobiol
PubMed ID7740075
Photolytic decomposition of sodium nitroprusside (SNP), a widely used nitrovasodilator, produced nitric oxide (.NO), which was continuously monitored by electron spin resonance (ESR) spectroscopy. The .NO present in the aqueous or the lipid phase was trapped by either a hydrophilic or a hydrophobic nitronyl nitroxide, respectively, to form the corresponding ... More
Nitric oxide-inducible expression of heme oxygenase-1 in human cells. Translation-independent stabilization of the mRNA and evidence for direct action of nitric oxide.
AuthorsBouton C, Demple B
JournalJ Biol Chem
PubMed ID11032845
Expression of heme oxygenase-1 (HO-1) in mammalian cells contributes to resistance to various types of free radical damage. Nitric oxide (NO) induces HO-1 in many cell types, but the specific contribution of transcriptional or post-transcriptional effects to this induction have remained unresolved. Here we show that the extent of HO-1 ... More
Involvement of oxidative stress in tumor cytotoxic activity of hepatocyte growth factor/scatter factor.
AuthorsArakaki N, Kajihara T, Arakaki R, Ohnishi T, Kazi JA, Nakashima H, Daikuhara Y
JournalJ Biol Chem
PubMed ID10224123
In this study, we show that N-acetylcysteine (NAC), a precursor of glutathione and an intracellular free radical scavenger, almost completely prevented hepatocyte growth factor (HGF)-suppressed growth of Sarcoma 180 and Meth A cells, and HGF-induced apoptosis, assessed by DNA fragmentation, and increase in caspase-3 activity, in Sarcoma 180 cells. The ... More
Differential modulation of prostaglandin H synthase-2 by nitric oxide-related species in intact cells.
Nitrogen monoxide (NO) has been reported to both activate and inhibit prostaglandin (PG) biosynthesis. This apparent paradox might be explained by the production/action of distinct NO-related species formed as a result of the prevailing redox states of different cellular systems. As such, the effect of NO donors with different redox ... More
Regulation of presynaptic phosphatidylinositol 4,5-biphosphate by neuronal activity.
AuthorsMicheva KD, Holz RW, Smith SJ
JournalJ Cell Biol
PubMed ID11470824
Phosphatidylinositol 4,5-biphosphate (PIP2) has been implicated in a variety of cellular processes, including synaptic vesicle recycling. However, little is known about the spatial distribution of this phospholipid in neurons and its dynamics. In this study, we have focused on these questions by transiently expressing the phospholipase C (PLC)-delta1 pleckstrin homology ... More
Inhibition of NF-kappaB and HIV-1 long terminal repeat transcriptional activation by inducible nitric oxide synthase 2 activity.
AuthorsSekkaï D, Aillet F, Israël N, Lepoivre M
JournalJ Biol Chem
PubMed ID9461573
In the human lymphoblastoid T cell line JJhan-5.1, stably transfected with a human immunodeficiency virus-1 long terminal repeat luciferase vector, the level of luciferase activity is dependent on activation of the nuclear factor kappaB (NF-kappaB) transcription factor. Tumor necrosis factor-induced luciferase activity was not modified in JJhan-5.1 cells co-cultivated with ... More
Mechanism of nitric oxide release from S-nitrosothiols.
AuthorsSingh RJ, Hogg N, Joseph J, Kalyanaraman B
JournalJ Biol Chem
PubMed ID8702510
S-Nitrosothiols have many biological activities and have been suggested to be intermediates in signal transduction. The mechanism and products of S-nitrosothiol decomposition are of great significance to the understanding of nitric oxide (.NO) biochemistry. S-Nitrosothiols are stable compounds at 37 degrees C and pH 7.4 in the presence of transition ... More
Therapeutic effects of imidazolineoxyl N-oxide against endotoxin shock through its direct nitric oxide-scavenging activity.
AuthorsYoshida M, Akaike T, Wada Y, Sato K, Ikeda K, Ueda S, Maeda H
JournalBiochem Biophys Res Commun
PubMed ID8048966
We recently found a new class of nitric oxide (NO) antidote, i.e., 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl-3-oxide derivatives (PTIOs). It has a potent inhibitory action against endothelium-derived relaxing factor. Here, we report the effect of a water-soluble carboxy derivative of PTIO (carboxy-PTIO) on endotoxin shock. Endotoxin [lipopolysaccharide (LPS)] (10 mg/kg) was injected into Wistar ... More
Modulation of white adipose tissue lipolysis by nitric oxide.
AuthorsGaudiot N, Jaubert AM, Charbonnier E, Sabourault D, Lacasa D, Giudicelli Y, Ribière C
JournalJ Biol Chem
PubMed ID9593681
In isolated adipocytes, the nitrosothiols S-nitroso-N-acetyl-penicillamine (SNAP) and S-nitrosoglutathione stimulate basal lipolysis, whereas the nitric oxide (NO.) donor 1-propamine, 3-(2-hydroxy-2-nitroso-1-propylhydrazine) (PAPA-NONOate) or NO gas have no effect. The increase in basal lipolysis due to nitrosothiols was prevented by dithiothreitol but not by a guanylate cyclase inhibitor. In addition the cyclic ... More
Scavenging effects of dopamine agonists on nitric oxide radicals.
AuthorsNishibayashi S, Asanuma M, Kohno M, Gómez-Vargas M, Ogawa N
JournalJ Neurochem
PubMed ID8863533
It has recently been considered that free radicals are closely involved in the pathogenesis of Parkinson's disease (PD), and the level of nitric oxide radical (.NO), one of the free radicals, is reported to increase in PD brain. In the present study, we established a direct detection system for .NO ... More
Nitric oxide plays a central role in determining lateral root development in tomato.
AuthorsCorrea-Aragunde N, Graziano M, Lamattina L
JournalPlanta
PubMed ID14716561
Nitric oxide (NO) is a bioactive molecule that functions in numerous physiological processes in plants, most of them involving cross-talk with traditional phytohormones. Auxin is the main hormone that regulates root system architecture. In this communication we report that NO promotes lateral root (LR) development, an auxin-dependent process. Application of ... More
Nuclear factor-kappa B and mitogen-activated protein kinases mediate nitric oxide-enhanced transcriptional expression of interferon-beta.
AuthorsJacobs AT, Ignarro LJ
JournalJ Biol Chem
PubMed ID12500976
Mitogen-activated protein (MAP) kinase and nuclear factor-kappaB (NF-kappaB) activation are critical for initiating the transcriptional expression of cytokines, cell adhesion molecules, and other factors in the macrophage immune response. Nitric oxide (NO), an endogenous free radical, is a product of macrophages that mediates inflammatory and cytotoxic processes in the immune ... More
Possible mechanism of bronchoprotection by SIN-1 in anaesthetized guinea pigs: roles of nitric oxide and peroxynitrite.
AuthorsKanazawa H, Hirata K, Yoshikawa J
JournalClin Exp Allergy
PubMed ID10691906
BACKGROUND: S-morpholinosydnonimine (SIN-1) is thought to generate peroxynitrite. Recent reports suggested that peroxynitrite possessed a potent vascular relaxant activity via guanylate cyclase activation. However, no previous studies have examined the relaxant effect of peroxynitrite on airway smooth muscle. OBJECTIVE: To determine the mechanism of bronchoprotection by SIN-1, considering in particular ... More
Melanin granules prevent the cytotoxic effects of L-DOPA on retinal pigment epithelial cells in vitro by regulation of NO and superoxide radicals.
AuthorsAkeo K, Amaki S, Suzuki T, Hiramitsu T
JournalPigment Cell Res
PubMed ID10841029
Inasmuch as the nitrogen cycle elicits the direct reduction of N2 to NH3 through enzymatic reactions, and inasmuch as L-DOPA (L-dihydroxyphentlalamine), a catecholamine, can be a source of nitric oxide (NO), it is possible that melanin granules in the eye affect the generation of NO, which causes damage to the ... More
Depletion of nitric oxide causes cell cycle alterations, apoptosis, and oxidative stress in pulmonary cells.
AuthorsJanssen YM, Soultanakis R, Steece K, Heerdt E, Singh RJ, Joseph J, Kalyanaraman B
JournalAm J Physiol
PubMed ID9843847
Nitric oxide (NO.) is important in the regulation of mitochondrial function, cell signaling, and gene expression. To elucidate how endogenous NO. regulates the function of airway epithelial cells, we used carboxy-PTIO, a hydrophilic, negatively charged NO. trap, to scavenge NO. from rat lung epithelial (RLE) and rat pleural mesothelial (RPM) ... More
Rate of deactivation of nitric oxide-stimulated soluble guanylate cyclase: influence of nitric oxide scavengers and calcium.
AuthorsMargulis A, Sitaramayya A
JournalBiochemistry
PubMed ID10653648
Soluble guanylate cyclase (sGC) is highly activated by nitric oxide (NO) and is the known mediator of the effects of NO on a variety of physiological processes. The rates at which sGC is activated and deactivated are therefore of wide interest since they determine the duration of a tissue's response ... More
Nitric oxide inhibits spinally projecting paraventricular neurons through potentiation of presynaptic GABA release.
AuthorsLi DP, Chen SR, Pan HL
JournalJ Neurophysiol
PubMed ID12424302
Nitric oxide (NO) in the paraventricular nucleus (PVN) is involved in the regulation of the excitability of PVN neurons. However, the effect of NO on the inhibitory GABAergic and excitatory glutamatergic inputs to spinally projecting PVN neurons has not been studied specifically. In the present study, we determined the role ... More
Endothelial heme oxygenase-1 induction by hypoxia. Modulation by inducible nitric-oxide synthase and S-nitrosothiols.
AuthorsMotterlini R, Foresti R, Bassi R, Calabrese V, Clark JE, Green CJ
JournalJ Biol Chem
PubMed ID10788478
The stress protein heme oxygenase-1 (HO-1) is induced in endothelial cells exposed to nitric oxide (NO)-releasing agents, and this process is finely modulated by thiols (Foresti, R., Clark, J. E., Green, C. J., and Motterlini R. (1997) J. Biol. Chem. 272, 18411-18417). Here, we report that up-regulation of HO-1 in ... More
Distinction between nitrosating mechanisms within human cells and aqueous solution.
AuthorsEspey MG, Miranda KM, Thomas DD, Wink DA
JournalJ Biol Chem
PubMed ID11404354
The quintessential nitrosating species produced during NO autoxidation is N(2)O(3). Nitrosation of amine, thiol, and hydroxyl residues can modulate critical cell functions. The biological mechanisms that control reactivity of nitrogen oxide species formed during autoxidation of nano- to micromolar levels of NO were examined using the synthetic donor NaEt(2)NN(O)NO (DEA/NO), ... More
Inhibitory effect of several nitric oxide-generating compounds on purified Na+,K(+)-ATPase activity from porcine cerebral cortex.
AuthorsSato T, Kamata Y, Irifune M, Nishikawa T
JournalJ Neurochem
PubMed ID9048779
Nitric oxide (NO)-generating compounds (NO donors) such as sodium nitroprusside, S-nitroso-N-acetylpenicillamine, S-nitroso-L-glutathione, 3-morpholino-sydnonimine (SIN-1), (DL)-(E)-ethyl-2-[(E)-hydroxyimino]-5-nitro-5-3-hexenamide, and 1-hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazene inhibited the Na+,K(+)-ATPase activity purified from porcine cerebral cortex. NO-reducing or -scavenging agents, such as superoxide dismutase or N-(dithiocarbamate)-N-methyl-D-glucamine sodium salt, L-ascorbic acid; and sulfhydryl (SH) compounds, such as dithiothreitol or the reduced ... More
Inactivation of glutathione peroxidase by nitric oxide. Implication for cytotoxicity.
AuthorsAsahi M, Fujii J, Suzuki K, Seo HG, Kuzuya T, Hori M, Tada M, Fujii S, Taniguchi N
JournalJ Biol Chem
PubMed ID7673130
S-nitro-N-acetyl-DL-penicillamine (SNAP), a nitric oxide (NO) donor, inactivated bovine glutathione peroxidase (GPx) in a dose- and time-dependent manner. The IC50 of SNAP for GPx was 2 microM at 1 h of incubation and was 20% of the IC50 for another thiol enzyme, glyceraldehyde-3-phosphate dehydrogenase, in which a specific cysteine residue ... More
A calcium-induced calcium influx factor, nitric oxide, modulates the refilling of calcium stores in astrocytes.
AuthorsLi N, Sul JY, Haydon PG
JournalJ Neurosci
PubMed ID14614089
The roles of nitric oxide are primarily undefined in astrocytes, cells that are active partners in synaptic transmission. Because nitric oxide synthases are present in astrocytes, we imaged the formation of nitric oxide in cultured murine cortical astrocytes using DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate). We demonstrated that physiological concentrations of ATP induced ... More
Reactive oxygen species-mediated regulation of eNOS and iNOS expression in multicellular prostate tumor spheroids.
AuthorsWartenberg M, Schallenberg M, Hescheler J, Sauer H
JournalInt J Cancer
PubMed ID12569550
Nitric oxide (NO) generated by either endothelial nitric oxide synthase (eNOS) or inducible nitric oxide synthase (iNOS) may be involved in prostate tumorigenesis through the inhibition of reactive oxygen species (ROS)-induced apoptosis. Multicellular DU-145 prostate tumor spheroids endogenously generated NO that paralleled the production of ROS. With increasing spheroid size, ... More