Functional expression of the murine Golgi CMP-sialic acid transporter in saccharomyces cerevisiae.
AuthorsBerninsone P, Eckhardt M, Gerardy-Schahn R, Hirschberg CB
JournalJ Biol Chem
PubMed ID9139716
'We have functionally expressed the murine Golgi putative CMP-sialic acid transporter in Saccharomyces cerevisiae. Using a galactose- inducible expression system, S. cerevisiae vesicles were able to transport CMP-sialic acid. Transport was dependent on galactose induction and was temperature-dependent and saturable with an apparent Km of 2.9 microM. Transport was inhibited ... More
I-PpoI, the endonuclease encoded by the group I intron PpLSU3, is expressed from an RNA polymerase I transcript.
AuthorsLin J, Vogt VM
JournalMol Cell Biol
PubMed ID9742098
'PpLSU3, a mobile group I intron in the rRNA genes of Physarum polycephalum, also can home into yeast chromosomal ribosomal DNA (rDNA) (D. E. Muscarella and V. M. Vogt, Mol. Cell. Biol. 13:1023-1033, 1993). By integrating PpLSU3 into the rDNA copies of a yeast strain temperature sensitive for RNA polymerase ... More
Highly stereoselective reagents for beta-keto ester reductions by genetic engineering of baker's yeast.
Authors RodrÃguez S; Kayser M M; Stewart J D;
JournalJ Am Chem Soc
PubMed ID11456752
While whole cells of baker's yeast (Saccharomyces cerevisiae) are a convenient biocatalytic reducing agent for a wide variety of carbonyl compounds, mixtures of stereoisomeric alcohols are often observed since the organism contains a large number of reductase enzymes with overlapping substrate specificities but differing stereoselectivities. We sought to improve the ... More
Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms.
A central question in lignin biosynthesis is how guaiacyl intermediates are hydroxylated and methylated to the syringyl monolignol in angiosperms. To address this question, we cloned cDNAs encoding a cytochrome P450 monooxygenase (LsM88) and a caffeate O-methyltransferase (COMT) from sweetgum (Liquidambar styraciflua) xylem. Mass spectrometry-based functional analysis of LsM88 in ... More
Desaturation and Hydroxylation. RESIDUES 148 AND 324 OF ARABIDOPSIS FAD2, IN ADDITION TO SUBSTRATE CHAIN LENGTH, EXERT A MAJOR INFLUENCE IN PARTITIONING OF CATALYTIC SPECIFICITY.
Authors Broadwater John A; Whittle Edward; Shanklin John;
JournalJ Biol Chem
PubMed ID11864983
Exchanging the identity of amino acids at four key locations within the Arabidopsis thaliana oleate desaturase (FAD2) and the Lesquerella fendleri hydroxylase/desaturase (LFAH) was shown to influence partitioning between desaturation and hydroxylation (Broun, P., Shanklin, J., Whittle, E., and Somerville, C. (1998) Science 282, 1315-1317). We report that four analogous ... More