Construction and application of a yeast surface-displayed human cDNA library to identify post-translational modification-dependent protein-protein interactions.
AuthorsBidlingmaier S, Liu B,
JournalMol Cell Proteomics
PubMed ID16321969
Although post-translational modifications such as phosphorylation mediate fundamental biological processes within the cell, relatively few methods exist that allow proteome-wide identification of proteins that interact with these modifications. We have constructed a yeast surface-displayed human cDNA library and utilized it to identify protein fragments with affinity for phosphorylated peptides derived ... More
Protective and therapeutic capacity of human single-chain Fv-Fc fusion proteins against West Nile virus.
AuthorsGould LH, Sui J, Foellmer H, Oliphant T, Wang T, Ledizet M, Murakami A, Noonan K, Lambeth C, Kar K, Anderson JF, de Silva AM, Diamond MS, Koski RA, Marasco WA, Fikrig E,
JournalJ Virol
PubMed ID16282460
West Nile virus has spread rapidly across the United States, and there is currently no approved human vaccine or therapy to prevent or treat disease. Passive immunization with antibodies against the envelope protein represents a promising means to provide short-term prophylaxis and treatment for West Nile virus infection. In this ... More
A general method for greatly improving the affinity of antibodies by using combinatorial libraries.
AuthorsRajpal A, Beyaz N, Haber L, Cappuccilli G, Yee H, Bhatt RR, Takeuchi T, Lerner RA, Crea R,
JournalProc Natl Acad Sci U S A
PubMed ID15939870
Look-through mutagenesis (LTM) is a multidimensional mutagenesis method that simultaneously assesses and optimizes combinatorial mutations of selected amino acids. The process focuses on a precise distribution within one or more complementarity determining region (CDR) domains and explores the synergistic contribution of amino acid side-chain chemistry. LTM was applied to an ... More