Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent, 0.1 mL - FAQs

View additional product information for Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent - FAQs (CMAX00008, CMAX00003, CMAX00015, CMAX00001)

6 product FAQs found

Can I use Lipofectamine CRISPRMAX Cas9 Transfection Reagent with one-cell embryos?

We have no data regarding the use of CRISPRMAX in one-celled embryos, therefore we do not recommend using this product for this application.

The shipping for my Lipofectamine CRISPRMAX Cas9 Transfection Reagent was delayed. Can I still use it?

All of the Lipofectamine reagents, including Lipofectamine CRISPRMAX Cas9 Transfection Reagent, are stable at room temperature for months. What is most important is that the reagent did not freeze, because that will alter the integrity and composition of the lipid particles and will compromise usability. Previous freezing can be evidenced by precipitation and excessive cloudiness of the reagent. A limited amount of cloudiness is normal, but excessive cloudiness is a sign that the reagent froze at some point. If the reagent you received looks clear or a bit cloudy the product should still be usable. Some additional data on room temperature stability of some Lipofectamine reagents, in the context of shipping, can be found in this white paper.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How much Lipofectamine CRISPRMAX Reagent, Cas9 protein, and gRNA should I complex for gene editing?

Please see the Lipofectamine CRISPRMAX Reagent manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014545_lipofectamine_crispermax_QR.pdf) for protocol details or contact techsupport@thermofisher.com.

How does the Lipofectamine CRISPRMAX Reagent work?

The Lipofectamine CRISPRMAX Reagent combined with the proprietary enhancement properties of the Lipofectamine Cas9 Plus Reagent leads to efficient complex formation with the Cas9-gRNA ribonucleoprotein, for best delivery to the nucleus, helping to ensure high gene editing frequency for a wide range of cell types.

Can the Lipofectamine CRISPRMAX Reagent be used to deliver proteins other than the Cas9 nuclease?

Although the Lipofectamine CRISPRMAX Reagent was developed for the delivery of the Cas9-gRNA ribonucleoprotein, there is potential for other protein complex applications.

Can the Lipofectamine CRISPRMAX Reagent also be used to deliver the donor plasmid in addition to the Cas9 nuclease protein and gRNA?

The Lipofectamine CRISPRMAX Reagent was developed to efficiently deliver the Cas9-gRNA ribonucleoprotein complex. We recommend that you first deliver the donor plasmid with Lipofectamine 3000, then follow with delivering the Cas9-gRNA complex with Lipofectamine CRISPRMAX Reagent for best editing efficiency.