Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX.
AuthorsYu X, Liang X, Xie H, Kumar S, Ravinder N, Potter J, de Mollerat du Jeu X, Chesnut JD,
JournalBiotechnol Lett
PubMed ID26892225
'To identify the best lipid nanoparticles for delivery of purified Cas9 protein and gRNA complexes (Cas9 RNPs) into mammalian cells and to establish the optimal conditions for transfection. Using a systematic approach, we screened 60 transfection reagents using six commonly-used mammalian cell lines and identified a novel transfection reagent (named ... More