Extracellular cathepsin K exerts antimicrobial activity and is protective against chronic intestinal inflammation in mice.
AuthorsSina C, Lipinski S, Gavrilova O, Aden K, Rehman A, Till A, Rittger A, Podschun R, Meyer-Hoffert U, Haesler R, Midtling E, Pütsep K, McGuckin MA, Schreiber S, Saftig P, Rosenstiel P,
JournalGut
PubMed ID22442160
'Cathepsin K is a lysosomal cysteine protease that has pleiotropic roles in bone resorption, arthritis, atherosclerosis, blood pressure regulation, obesity and cancer. Recently, it was demonstrated that cathepsin K-deficient (Ctsk(-/-) ) mice are less susceptible to experimental autoimmune arthritis and encephalomyelitis, which implies a functional role for cathepsin K in ... More
Cytosolic phospholipase a2 activation by Candida albicans in alveolar macrophages: role of dectin-1.
AuthorsParti RP, Loper R, Brown GD, Gordon S, Taylor PR, Bonventre JV, Murphy RC, Williams DL, Leslie CC,
JournalAm J Respir Cell Mol Biol
PubMed ID19502385
Candida albicans is an increasingly important pulmonary fungal pathogen. Resident alveolar macrophages are important in host defense against opportunistic fungal infections. Activation of Group IVA cytosolic phospholipase A(2)alpha (cPLA(2)alpha) in macrophages initiates arachidonic acid (AA) release for production of eicosanoids, which regulate inflammation and immune responses. We investigated the ability ... More
Selective requirement for CD40-CD154 in drug-induced type 1 versus type 2 responses to trinitrophenyl-ovalbumin.
AuthorsNierkens S, van Helden P, Bol M, Bleumink R, van Kooten P, Ramdien-Murli S, Boon L, Pieters R,
JournalJ Immunol
PubMed ID11937525
CD154 is transiently expressed by activated T cells and interacts with CD40 on B cells, dendritic cells, macrophages, and monocytes. This costimulatory receptor-ligand couple seems decisive in Ag-driven immune responses but may be differentially involved in type 1 vs type 2 responses. We studied the importance of CD40-CD154 in both ... More
Chlamydia psittaci genetic variants differ in virulence by modulation of host immunity.
AuthorsMiyairi I, Laxton JD, Wang X, Obert CA, Arva Tatireddigari VR, van Rooijen N, Hatch TP, Byrne GI,
JournalJ Infect Dis
PubMed ID21791668
Psittacosis is a zoonosis caused by Chlamydia psittaci and is characterized by severe pneumonia and systemic infection. We sought to determine the basis of the 1000-fold difference in lethal dose of 2 C. psittaci 6BC strains in mice. Genomes of the strains were sequenced. Mice were infected intraperitoneally and the ... More
Control of experimental Trypanosoma brucei infections occurs independently of lymphotoxin-alpha induction.
Trypanosome infections are marked by severe pathological features, including anemia, splenomegaly, and suppression of T-cell proliferation. We have used lymphotoxin-alpha-deficient (LT-alpha(-/-)) mice, as well as LT-alpha-tumor necrosis factor-double-deficient (LT-alpha(-/-) TNF(-/-)) mice, to analyze the contributions of these related cytokines in both induction of trypanosomosis-associated immunopathology and infection control. Moreover, as ... More
Overcoming immunoescape mechanisms of BCL1 leukemia and induction of CD8+ T-cell-mediated BCL1-specific resistance in mice cured by targeted polymer-bound doxorubicin.
BALB/c mice bearing syngeneic BCL1 leukemia, a mouse model of human chronic lymphocytic leukemia, were treated with polymer-bound doxorubicin conjugate targeted with BCL1-specific monoclonal antibody. Such treatment can cure up to 100% of mice and the cured mice show long-lasting resistance to BCL1 leukemia. We show that both CD4+ and ... More
Diminished cytokine and chemokine expression in the central nervous system of GMF-deficient mice with experimental autoimmune encephalomyelitis.
AuthorsZaheer A, Sahu SK, Wu Y, Zaheer A, Haas J, Lee K, Yang B,
JournalBrain Res
PubMed ID17316572
Pro-inflammatory cytokines/chemokines are implemented in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model with clinical and pathological similarities to multiple sclerosis. We have previously shown that over-expression of glia maturation factor (GMF) in glial cells cause excessive production and secretion of pro-inflammatory cytokines/chemokines sufficient to destroy the myelin-forming ... More
Glia maturation factor regulation of STAT expression: a novel mechanism in experimental autoimmune encephalomyelitis.
AuthorsZaheer S, Wu Y, Bassett J, Yang B, Zaheer A,
JournalNeurochem Res
PubMed ID17551829
Inflammatory cytokines are implemented in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. We previously demonstrated that glia maturation factor (GMF), a brain protein, isolated, sequenced and cloned in our laboratory, induce expression of proinflammatory cytokine/chemokine in the central nervous system (CNS). We found GMF-deficient ... More
A novel dendritic cell-induced model of erosive inflammatory arthritis: distinct roles for dendritic cells in T cell activation and induction of local inflammation.
AuthorsLeung BP, Conacher M, Hunter D, McInnes IB, Liew FY, Brewer JM
JournalJ Immunol
PubMed ID12471143
Transferring collagen-pulsed, bone marrow-derived dendritic cells (DCs) into congenic DBA/1 recipient mice produced arthritis in joints adjacent to the site of DC transfer and could be inhibited by treatment with TNF antagonists. Disease was Ag specific, as transfer of control, unpulsed DCs, or DCs pulsed with OVA did not produce ... More