Immature rat myeloid dendritic cells generated in low-dose granulocyte macrophage-colony stimulating factor prolong donor-specific rat cardiac allograft survival.
AuthorsDePaz HA, Oluwole OO, Adeyeri AO, Witkowski P, Jin MX, Hardy MA, Oluwole SF,
JournalTransplantation
PubMed ID12605121
'Because the differential polarization of T cells in response to antigen presentation is dependent on the maturational state of dendritic cells (DCs), we hypothesized that the adoptive transfer of immature myeloid DCs (iMDCs) would prolong graft survival. To evaluate this hypothesis, we studied the effects of transfer of iMDCs and ... More
Biological characterization of a novel class of toll-like receptor 7 agonists designed to have reduced systemic activity.
AuthorsBiffen M, Matsui H, Edwards S, Leishman AJ, Eiho K, Holness E, Satterthwaite G, Doyle I, Wada H, Fraser NJ, Hawkins SL, Aoki M, Tomizawa H, Benjamin AD, Takaku H, McInally T, Murray CM,
JournalBr J Pharmacol
PubMed ID22122192
'BACKGROUND AND PURPOSE Toll-like receptor 7 (TLR7) agonists have potential in the treatment of allergic diseases. However, the therapeutic utility of current low molecular weight TLR7 agonists is limited by their systemic activity, resulting in unwanted side effects. We have developed a series of TLR7-selective ''antedrugs'', including SM-324405 and AZ12441970, ... More
Wheat gliadins modified by deamidation are more efficient than native gliadins in inducing a Th2 response in Balb/c mice experimentally sensitized to wheat allergens.
'Wheat gluten proteins such as gliadins constitute major food allergens. Gluten can be modified industrially by deamidation which increases its solubility and enhances its use as a food ingredient. Sensitization to deamidated gluten has been reported to cause severe allergic reactions with anaphylaxis. The aim of this study was therefore ... More
Differential effects of fucoidans with low and high molecular weight on the viability and function of spleen cells.
AuthorsJang JY, Moon SY, Joo HG,
Journal
PubMed ID24681238
Fucoidan is an edible sulfated polysaccharide purified from brown algae that has multiple biological activities. However, the effects of fucoidans of different molecular weights on immune cells have not been determined. Thus, we treated spleen cells with low- and high-molecular-weight fucoidans (LMF and HMF, respectively). Viability assays demonstrated that HMF ... More
Immunological and metabolomic impacts of administration of Cry1Ab protein and MON 810 maize in mouse.
AuthorsAdel-Patient K, Guimaraes VD, Paris A, Drumare MF, Ah-Leung S, Lamourette P, Nevers MC, Canlet C, Molina J, Bernard H, Créminon C, Wal JM,
JournalPLoS One
PubMed ID21298004
We have investigated the immunological and metabolomic impacts of Cry1Ab administration to mice, either as a purified protein or as the Cry1Ab-expressing genetically modified (GM) MON810 maize. Humoral and cellular specific immune responses induced in BALB/cJ mice after intra-gastric (i.g.) or intra-peritoneal (i.p.) administration of purified Cry1Ab were analyzed and ... More
Control of experimental Trypanosoma brucei infections occurs independently of lymphotoxin-alpha induction.
Trypanosome infections are marked by severe pathological features, including anemia, splenomegaly, and suppression of T-cell proliferation. We have used lymphotoxin-alpha-deficient (LT-alpha(-/-)) mice, as well as LT-alpha-tumor necrosis factor-double-deficient (LT-alpha(-/-) TNF(-/-)) mice, to analyze the contributions of these related cytokines in both induction of trypanosomosis-associated immunopathology and infection control. Moreover, as ... More
Perinatal and postweaning exposure to galactooligosaccharides/inulin prebiotics induced biomarkers linked to tolerance mechanism in a mouse model of strong allergic sensitization.
AuthorsGourbeyre P, Desbuards N, Grémy G, Tranquet O, Champ M, Denery-Papini S, Bodinier M,
Journal
PubMed ID23746232
Food allergies are increasing, and no treatment exists, thus enhancing interest in prebiotic strategies. This study aimed to analyze the preventive effects of prebiotic feeding during perinatal and postweaning periods in a mouse model of allergy by studying biomarkers related to tolerance (IgG2a, IgA, IFN-?, TGF-ß, and IL-10), to allergy ... More
Overcoming immunoescape mechanisms of BCL1 leukemia and induction of CD8+ T-cell-mediated BCL1-specific resistance in mice cured by targeted polymer-bound doxorubicin.
BALB/c mice bearing syngeneic BCL1 leukemia, a mouse model of human chronic lymphocytic leukemia, were treated with polymer-bound doxorubicin conjugate targeted with BCL1-specific monoclonal antibody. Such treatment can cure up to 100% of mice and the cured mice show long-lasting resistance to BCL1 leukemia. We show that both CD4+ and ... More
Diminished cytokine and chemokine expression in the central nervous system of GMF-deficient mice with experimental autoimmune encephalomyelitis.
AuthorsZaheer A, Sahu SK, Wu Y, Zaheer A, Haas J, Lee K, Yang B,
JournalBrain Res
PubMed ID17316572
Pro-inflammatory cytokines/chemokines are implemented in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model with clinical and pathological similarities to multiple sclerosis. We have previously shown that over-expression of glia maturation factor (GMF) in glial cells cause excessive production and secretion of pro-inflammatory cytokines/chemokines sufficient to destroy the myelin-forming ... More
Glia maturation factor regulation of STAT expression: a novel mechanism in experimental autoimmune encephalomyelitis.
AuthorsZaheer S, Wu Y, Bassett J, Yang B, Zaheer A,
JournalNeurochem Res
PubMed ID17551829
Inflammatory cytokines are implemented in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. We previously demonstrated that glia maturation factor (GMF), a brain protein, isolated, sequenced and cloned in our laboratory, induce expression of proinflammatory cytokine/chemokine in the central nervous system (CNS). We found GMF-deficient ... More