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View additional product information for ChargeSwitch™ gDNA Normalized Buccal Cell Kit - FAQs (CS11020, CS1102010)
9 product FAQs found
我们强烈建议使用试剂盒提供的洗脱缓冲液，并且不建议用水进行洗脱。如果你需要使用任何其它的缓冲液进行洗脱，请确保使用pH 8.5–9.0的缓冲液以实现DNA的高效洗脱。
带filter的试剂盒是基于一个双巢式柱的设计，它能够有助于通过最快最简便的步骤获得高质量的质粒（ (<10 EU/µg DNA）。经过改良的缓冲液1可去除更多内毒素。
通常而言ChargeSwitch Pro mini试剂盒从5mL菌液可得到25 µg质粒，midi试剂盒从25 mL菌液可得到200µg质粒，而 maxi试剂盒从100 mL菌液可得到800 µg质粒。试剂盒可以纯化的质粒大小范围通常是3–9 kb。
我们推荐使用MagMAX DNA Multi-Sample Ultra 2.0试剂盒或ChargeSwitch 口腔细胞基因组DNA试剂盒（https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/buccal-dna-extraction.html），这款产品专用于从口腔细胞样本中分离高品质的基因组DNA。同时我们也提供标准化试剂盒（normalized kits），确保用户针对不同样品分离所得的DNA浓度进行标准化处理。
We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.
The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.
The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.
We would recommend using one of our MagMAX DNA Multi-Sample Ultra 2.0 Kit or ChargeSwitch gDNA Buccal Cell Kits, which were designed to isolate high-quality genomic DNA from buccal samples. We also offer normalized kits, which will normalize isolated DNA concentration from sample to sample.
Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.