ChargeSwitch™ 裂解缓冲液 L13
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Invitrogen™

ChargeSwitch™ 裂解缓冲液 L13

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The ChargeSwitch® Forensic DNA Purification Kit is a magnetic bead method designed to accommodate all forensic crime scene sample types了解更多信息
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货号数量
CS11202800 mL
货号 CS11202
价格(CNY)
1,252.00
Each
添加至购物车
数量:
800 mL
价格(CNY)
1,252.00
Each
添加至购物车
The ChargeSwitch® Forensic DNA Purification Kit is a magnetic bead method designed to accommodate all forensic crime scene sample types for STR profiling. The kit is scalable for single-tube use or for automated extraction.
仅供科研使用。不可用于诊断程序。
规格
缓冲液裂解缓冲液
适用于(应用)DNA 提取
环保功能所用资源更少、废弃物更少、危害更小
产品线ChargeSwitch™
产品类型裂解缓冲液 L13
数量800 mL
运输条件室温
产品规格试剂盒
Unit SizeEach
内容与储存
ChargeSwitch™ 法医 DNA 纯化试剂盒的所有组分均在室温下运输。接收后,将蛋白酶 K 储存在 4C 下。将所有其他组分储存在室温下。如果妥善储存,可保证所有组分稳定储存 6 个月。

常见问题解答 (FAQ)

使用ChargeSwitch试剂盒时我用水替代洗脱缓冲液,但是得到非常低的质粒产量。这是为什么?

我们强烈建议使用试剂盒提供的洗脱缓冲液,并且不建议用水进行洗脱。如果你需要使用任何其它的缓冲液进行洗脱,请确保使用pH 8.5–9.0的缓冲液以实现DNA的高效洗脱。

ChargeSwitch Pro试剂盒和Charge-Switch Pro filter试剂盒的区别是什么?

带filter的试剂盒是基于一个双巢式柱的设计,它能够有助于通过最快最简便的步骤获得高质量的质粒( (<10 EU/µg DNA)。经过改良的缓冲液1可去除更多内毒素。

ChargeSwitch Pro Filter Plasmid Mini 或 Midi试剂盒纯化质粒的常规产量是多少?

通常而言ChargeSwitch Pro mini试剂盒从5mL菌液可得到25 µg质粒,midi试剂盒从25 mL菌液可得到200µg质粒,而 maxi试剂盒从100 mL菌液可得到800 µg质粒。试剂盒可以纯化的质粒大小范围通常是3–9 kb。

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.