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ChargeSwitch™ gDNA Mini Bacteria Kit - FAQs

查看更多产品信息 ChargeSwitch™ gDNA Mini Bacteria Kit - FAQs (CS11301)

9 个常见问题解答

使用ChargeSwitch试剂盒时我用水替代洗脱缓冲液，但是得到非常低的质粒产量。这是为什么？

我们强烈建议使用试剂盒提供的洗脱缓冲液，并且不建议用水进行洗脱。如果你需要使用任何其它的缓冲液进行洗脱，请确保使用pH 8.5–9.0的缓冲液以实现DNA的高效洗脱。

ChargeSwitch Pro试剂盒和Charge-Switch Pro filter试剂盒的区别是什么？

带filter的试剂盒是基于一个双巢式柱的设计，它能够有助于通过最快最简便的步骤获得高质量的质粒（ (<10 EU/µg DNA）。经过改良的缓冲液1可去除更多内毒素。

ChargeSwitch Pro Filter Plasmid Mini 或 Midi试剂盒纯化质粒的常规产量是多少？

通常而言ChargeSwitch Pro mini试剂盒从5mL菌液可得到25 µg质粒，midi试剂盒从25 mL菌液可得到200µg质粒，而 maxi试剂盒从100 mL菌液可得到800 µg质粒。试剂盒可以纯化的质粒大小范围通常是3–9 kb。

我想分离细菌的基因组DNA，该如何操作？

我们推荐使用我们的PureLink基因组DNA小提试剂盒或ChargeSwitch 细菌基因组DNA小提试剂盒（货号CS11301），两款试剂盒均能够用于提取革兰氏阳性和革兰氏阴性的细菌样本。这种简易的单管操作方案无需离心或过滤步骤。请点击此处（https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/bacteria-dna-extraction.html）了解更多关于细菌DNA提取的信息。

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

I want to isolate genomic DNA from bacteria. How do I do this?

We would recommend using our PureLink gDNA Mini Kit or our ChargeSwitch gDNA Mini Bacteria Kit (Cat. No. CS11301), which can isolate both gram-positive and gram-negative bacteria. No centrifugation or filtration steps are necessary using this simple one-tube protocol. Read more about bacterial DNA extraction here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/bacteria-dna-extraction.html).

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.