Q: ChargeSwitch Pro Filter Plasmid Mini 或 Midi试剂盒纯化质粒的常规产量是多少？

通常而言ChargeSwitch Pro mini试剂盒从5mL菌液可得到25 µg质粒，midi试剂盒从25 mL菌液可得到200µg质粒，而 maxi试剂盒从100 mL菌液可得到800 µg质粒。试剂盒可以纯化的质粒大小范围通常是3–9 kb。

Q: 我该使用哪种试剂盒来分离血液基因组DNA？

请浏览此网页（https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/blood-dna-extraction.html），该网页介绍了从血液中分离基因组DNA的各种试剂盒的特点。

Q: I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

Q: What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

Q: What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

Q: What kit should I use for blood genomic DNA isolation?

Please see the following web page (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/blood-dna-extraction.html) for a comparison of kits that can be used to isolate genomic DNA from blood.

Question 1

使用ChargeSwitch试剂盒时我用水替代洗脱缓冲液，但是得到非常低的质粒产量。这是为什么？

Accepted Answer

我们强烈建议使用试剂盒提供的洗脱缓冲液，并且不建议用水进行洗脱。如果你需要使用任何其它的缓冲液进行洗脱，请确保使用pH 8.5–9.0的缓冲液以实现DNA的高效洗脱。

Question 2

ChargeSwitch Pro试剂盒和Charge-Switch Pro filter试剂盒的区别是什么？

Accepted Answer

带filter的试剂盒是基于一个双巢式柱的设计，它能够有助于通过最快最简便的步骤获得高质量的质粒（ (<10 EU/µg DNA）。经过改良的缓冲液1可去除更多内毒素。

Question 3

ChargeSwitch Pro Filter Plasmid Mini 或 Midi试剂盒纯化质粒的常规产量是多少？

Accepted Answer

通常而言ChargeSwitch Pro mini试剂盒从5mL菌液可得到25 µg质粒，midi试剂盒从25 mL菌液可得到200µg质粒，而 maxi试剂盒从100 mL菌液可得到800 µg质粒。试剂盒可以纯化的质粒大小范围通常是3–9 kb。

Question 4

我该使用哪种试剂盒来分离血液基因组DNA？

Accepted Answer

请浏览此网页（https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/blood-dna-extraction.html），该网页介绍了从血液中分离基因组DNA的各种试剂盒的特点。

Question 5

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

Accepted Answer

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

Question 6

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

Accepted Answer

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/&micro;g DNA). Modified wash buffer 1 removes more endotoxin.

Question 7

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

Accepted Answer

The typical yield is 25 &micro;g from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 &micro;g from a 25 mL culture for the midi kit, and 800 &micro;g from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

Question 8

What kit should I use for blood genomic DNA isolation?

Accepted Answer