SOCS2 influences LPS induced human monocyte-derived dendritic cell maturation.
AuthorsHu J, Winqvist O, Flores-Morales A, Wikström AC, Norstedt G
JournalPLoS One
PubMed ID19779605
'Dendritic cells (DCs) are highly specific antigen presenting cells, which link innate and adaptive immune responses and participate in protecting hosts from invading pathogens. DCs can be generated in vitro by culturing human monocytes with GM-CSF and IL-4 followed by LPS induced DC maturation. We set out to study the ... More
Activation of cryptic IgG reactive with BAFF, amyloid beta peptide and GM-CSF during the industrial fractionation of human plasma into therapeutic intravenous immunoglobulins.
AuthorsSt-Amour I, Laroche A, Bazin R, Lemieux R
JournalClin Immunol
PubMed ID19604724
'The mechanisms of therapeutic action of IVIg are still unclear in most autoimmune and inflammatory diseases. IVIg have been shown to bind to a variety of human proteins including BAFF, amyloid beta peptide and GM-CSF. It has been suggested that this autoreactivity could contribute to the therapeutic immunomodulatory effects of ... More
Lentiviral vector transduction of dendritic cells for novel vaccine strategies.
AuthorsChang LJ
JournalMethods Mol Biol
PubMed ID20225043
'Dendritic cells (DCs) are key antigen-presenting cells that induce primary and memory immune response. Patients with chronic infections or cancer often display DC dysfunctions. Modification of DCs or DC progenitors in vitro may overcome the problems with defective DCs in vivo. Lentiviral vector is highly efficient in transducing hematopoietic cells ... More
LPS regulates SOCS2 transcription in a type I interferon dependent autocrine-paracrine loop.
AuthorsHu J, Lou D, Carow B, Winerdal ME, Rottenberg M, Wikström AC, Norstedt G, Winqvist O
JournalPLoS One
PubMed ID22291912
'Recent studies suggest that SOCS2 is involved in the regulation of TLR signaling. In this study, we found that the expression of SOCS2 is regulated in human monocyte-derived DC by ligands stimulating TLR2, 3, 4, 5, 8 and 9 signaling. SOCS2 induction by LPS was dependent on the type I ... More
In vitro-generated DC with tolerogenic functions: perspectives for in vivo cellular therapy.
Authorsvan Kooten C, Gelderman KA
JournalMethods Mol Biol
PubMed ID20941608
Dendritic cells (DCs) have a central role in immune regulation and serve as an essential link between innate and adaptive immunity. Their broad range of powerful immune stimulatory as well as regulatory functions has made DCs a target for vaccine development strategies. One approach to promote the tolerogenicity of DCs ... More
Induction of primary anti-HIV CD4 and CD8 T cell responses by dendritic cells transduced with self-inactivating lentiviral vectors.
AuthorsChen X, Wang B, Chang LJ,
JournalCell Immunol
PubMed ID17188256
In this study, we demonstrate that a minimal self-inactivating (SIN) lentiviral vector (LV) that does not encode any human immunodeficiency virus (HIV) genes is able to induce HIV-specific CD4 and CD8 T cell responses after transduction of dendritic cells (DCs). The LV-DC-primed T cells displayed HIV-specific lytic degranulation, as illustrated ... More
TGF-beta1 dampens the susceptibility of dendritic cells to environmental stimulation, leading to the requirement for danger signals for activation.
AuthorsOhtani T, Mizuashi M, Nakagawa S, Sasaki Y, Fujimura T, Okuyama R, Aiba S
JournalImmunology
PubMed ID19278421
In contrast to its favourable effects on Langerhans cell (LC) differentiation, transforming growth factor (TGF)-beta1 has been reported to prevent dendritic cells from maturing in response to tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, or lipopolysaccharide (LPS). We first characterized the effects of TGF-beta1 on dendritic cell function by testing the ... More