PAF-acetylhydrolase expressed during megakaryocyte differentiation inactivates PAF-like lipids.
AuthorsFoulks JM, Marathe GK, Michetti N, Stafforini DM, Zimmerman GA, McIntyre TM, Weyrich AS
JournalBlood
PubMed ID19395675
'Platelet activating factor (PAF) and PAF-like lipids induce inflammatory responses in target cells. These lipid mediators are inactivated by PAF-acetylhydrolase (PAF-AH). The PAF signaling system affects the growth of hematopoietic CD34(+) cells, but roles for PAF-AH in this process are unknown. Here, we investigated PAF-AH function during megakaryopoiesis and found ... More
Correlations of cell surface antigens with individual differences in radiosensitivity in human hematopoietic stem/progenitor cells.
AuthorsTakahashi K, Monzen S, Hayashi N, Kashiwakura I
JournalRadiat Res
PubMed ID20095850
'To characterize the differences in the radiosensitivity of individual populations of human hematopoietic stem/progenitor cells (HSPCs), we examined the relationship among cell surface antigens, clonogenic potential and radiation survival. The expressions of CD34, CD38, CD45RA, CD110 and Tie-2, early differentiation pathway-related antigens in hematopoiesis, were analyzed on the surface of ... More
Radiosensitivity of human haematopoietic stem/progenitor cells.
AuthorsKato K, Omori A, Kashiwakura I
JournalJ Radiol Prot
PubMed ID23295782
'The haematopoietic system is regenerative tissue with a high proliferative potential; therefore, haematopoietic stem cells (HSCs) are sensitive to extracellular oxidative stress caused by radiation and chemotherapeutic agents. An understanding of this issue can help predict haematopoietic recovery from radiation exposure as well as the extent of radiation damage to ... More
Immobilization, stabilization, and activation of human stem cell factor (SCF) on fragmin/protamine microparticle (F/P MP)-coated plates.
AuthorsKishimoto S, Oonuma F, Nakamura S, Hattori H, Nakamura S, Mori Y, Tanaka Y, Harada Y, Tagawa M, Ishihara M
JournalJ Biomed Mater Res B Appl Biomater
PubMed ID19637375
'Fragmin (low-molecular-weight heparin)/protamine microparticles (F/P MPs) immobilize to culture plates, thereby retaining the binding of heparin-binding cytokines such as human stem cell factor (SCF). The purpose of this study was to evaluate the ability of F/P MP-coating to immobilize, stabilize, and enhance SCF-activity. Cell assays showed that SCF and preimmobilized ... More
The HIF-1 transcription complex is essential for translational control of myeloid hematopoietic cell function by maintaining mTOR phosphorylation.
Mammalian myeloid cells are crucial effectors of host innate immune defense. Normal and pathological responses of these cells require adaptation to signaling stress through the hypoxia-inducible factor 1 (HIF-1) transcription complex. Adapted cells activate the mammalian target of rapamycin (mTOR), via S2448 phosphorylation, which induces de novo translation of vital ... More
Crosstalk between mast cells and pancreatic cancer cells contributes to pancreatic tumor progression.
AuthorsStrouch MJ, Cheon EC, Salabat MR, Krantz SB, Gounaris E, Melstrom LG, Dangi-Garimella S, Wang E, Munshi HG, Khazaie K, Bentrem DJ,
JournalClin Cancer Res
PubMed ID20371681
To assess the clinical and pathologic significance of mast cell infiltration in human pancreatic cancer and evaluate crosstalk between mast cells and cancer cells in vitro. Immunohistochemistry for tryptase was done on 53 pancreatic cancer specimens. Mast cell counts were correlated with clinical variables and survival. Serum tryptase activity from ... More
IL-4 amplifies the pro-inflammatory effect of adenosine in human mast cells by changing expression levels of adenosine receptors.
Adenosine inhalation produces immediate bronchoconstriction in asthmatics but not in normal subjects. The bronchospastic effect of adenosine is largely mediated through adenosine-induced mast cell activation, the mechanism of which is poorly understood due to limitations in culturing human primary mast cells. Here, we show that human umbilical cord blood -derived ... More
Depression of progenitor cell function by advanced glycation endproducts (AGEs): potential relevance for impaired angiogenesis in advanced age and diabetes.
AuthorsScheubel RJ, Kahrstedt S, Weber H, Holtz J, Friedrich I, Borgermann J, Silber RE, Simm A
JournalExp Gerontol
PubMed ID16515851
Diabetes and ageing induce reduction and dysfunction of vascular progenitor cells. Advanced glycation endproducts (AGEs) accumulate in diabetes and ageing. We investigated the influence of AGEs on function of CD34 progenitor cells. CD34 cells were co-cultured with HUVECs in a three-dimensional spheroid assay. Sprout length growth and incorporation of CD34 ... More
Glycomic analysis of human mast cells, eosinophils and basophils.
AuthorsNorth SJ, von Gunten S, Antonopoulos A, Trollope A, MacGlashan DW, Jang-Lee J, Dell A, Metcalfe DD, Kirshenbaum AS, Bochner BS, Haslam SM
JournalGlycobiology
PubMed ID21725073
In allergic diseases such as asthma, eosinophils, basophils and mast cells, through release of preformed and newly generated mediators, granule proteins and cytokines, are recognized as key effector cells. While their surface protein phenotypes, mediator release profiles, ontogeny, cell trafficking and genomes have been generally explored and compared, there has ... More
miR-363-5p regulates endothelial cell properties and their communication with hematopoietic precursor cells.
AuthorsCosta A, Afonso J, Osório C, Gomes AL, Caiado F, Valente J, Aguiar SI, Pinto F, Ramirez M, Dias S
Journal
PubMed ID24257019
Recent findings have shown that the blood vessels of different organs exert an active role in regulating organ function. In detail, the endothelium that aligns the vasculature of most organs is fundamental in maintaining organ homeostasis and in promoting organ recovery following injury. Mechanistically, endothelial cells (EC) of tissues such ... More